- Open Access
TC003132 is essential for the follicle stem cell lineage in telotrophic Tribolium oogenesis
© The Author(s). 2017
Received: 27 March 2017
Accepted: 11 May 2017
Published: 19 May 2017
Stem cells are undifferentiated cells with a potential for self-renewal, which are essential to support normal development and homeostasis. To gain insight into the molecular mechanisms underlying adult stem cell biology and organ evolution, we use the telotrophic ovary of the beetle Tribolium. To this end, we participated in a large-scale RNAi screen in the red flour beetle Tribolium, which identified functions in embryonic and postembryonic development for more than half of the Tribolium genes.
We identified TC003132 as candidate gene for the follicle stem cell linage in telotrophic Tribolium oogenesis. TC003132 belongs to the Casein Kinase 2 substrate family (CK2S), which in humans is associated with the proliferative activity of different cancers. Upon TC003132 RNAi, central pre-follicular cells are lost, which results in termination of oogenesis. Given that also Notch-signalling is required to promote the mitotic activity of central pre-follicular cells, we performed epistasis experiments with Notch and cut. In addition, we identified a putative follicle stem cell population by monitoring the mitotic pattern of wild type and TC003132 depleted follicle cells by EdU incorporations. In TC003132 RNAi these putative FSCs cease the expression of differentiation makers and are eventually lost.
TC003132 depleted pre-follicular cells neither react to mitosis or endocycle stimulating signals, suggesting that TC003132 provides competence for differentiation cues. This may resemble the situation in C. elegans were CK2 is required to maintain the balance between proliferation and differentiation in the germ line. Since the earliest effect of TC003132 RNAi is characterized by the loss of putative FSCs, we posit that TC003132 crucially contributes to the proliferation or maintenance of follicle stem cells in the telotrophic Tribolium ovary.
In Drosophila, germline and follicle stem cells work in a coordinated fashion to produce egg chambers [1, 2]. Two to three germline stem cells (GSC) reside at the anterior tip of the germarium and divide asymmetrically to give rise to a cystoblast, which undergoes four mitotic divisions with incomplete cytokinesis to form an interconnected 16-cell cluster. One of these 16 cells is specified as an oocyte and the other cells differentiate into polyploid nurse cells that support the growth of the oocyte. These clusters are engulfed in the germarium by stromal escort cells (EC) until they reach the region 2a/2b border where the two somatic follicle stem cells (FSC) are located, which are maintained in part by niche signals from escort cells [3–5]. Initially, FSC daughter cells are unspecified and have the potential to either cross-migrate to the opposite side and replace the other stem cell or differentiate into either main body or polar/stalk precursors [6–8]. Notch, as well as JAK-STAT signalling further subdivides the stalk/polar pre-follicular cell linage in a stepwise manner [9–11]. Polar cell fate is induced in a restricted subset of this population by the Notch ligand Delta, which is produced in germline cells and induces the adjacent anterior follicle cells to differentiate into polar cells. These cells subsequently express the JAK/STAT ligand Unpaired, inducing stalk cell fates [9, 12]. Therefore three distinct follicle cell populations are present after follicle budding: polar cells which act as signalling centres, stalk cells that connect adjacent egg chambers, and main-body follicle cells which form an epithelium overlying the germline cyst .
In contrast to polytrophic-meroistic oogenesis represented by Drosophila, in telotrophic-meroistic Tribolium oogenesis oocytes and nurse cells of a germ cell cluster separate in a way, in which each follicle contains only one germ cell, the oocyte. Oocytes remain connected to the tropharium – a syncytium of nurse cells – by a nutritive cord . In Tribolium, germline proliferation is restricted to larval and early pupal stages, whereas the FSC niche remains active up to adulthood . Thus, the formation and maintenance of the follicle stem cell (FSC) linage in Tribolium is largely independent of the germline stem cells (GSCs).
In the Tribolium ovary, arrested pro-oocytes are arranged around the somatic plug, a group of small somatic cells located at the posterior end of the tropharium. Upon maturation, pro-oocytes separate from the somatic plug and enter the vitellarium, where they come in contact with pre-follicular cells, which successively encapsulate the oocyte to form an egg-chamber [15, 16]. Previously, we showed that Notch-signalling is required for encapsulation and early steps in follicle cell patterning, i.e. the determination of terminal follicle cells . Subsequently, graded levels of JAK-STAT signalling specify additional follicle sub-populations, including stalk precursor cells. Upon JAK-STAT RNAi, stalk cells are absent and anterior and posterior follicle cells of adjacent vitellogenic egg chambers maximise their area of contact, resulting in severe deformation of follicles . During pre-vitellogenic growth, Tribolium oocytes increase in size, while follicle cells divide to form a uniform epithelium surrounding the oocyte . Subsequently, follicle cells enter endocycle and eventually secrete the eggshells. However, in contrast to Drosophila where a Notch signal induces the follicle cells to leave mitosis [17, 18], in Tribolium egg-chambers Notch signalling prevents follicle cells from entering endocycle prematurely. Hence, with respect to the cycle/endocycle switch, Notch-signalling in Tribolium and Drosophila has opposing effects . While polytrophic and telotrophic oogenesis may involve the stepwise specification of follicle cell populations in a JAK-STAT and Notch dependent manner [15, 16], the regulatory mechanisms that determine and maintain the follicle stem cell lineage in telotrophic Tribolium oogenesis remains to elucidated.
In order to gain additional insights into the molecular mechanisms underlying telotrophic oogenesis and somatic stem cell biology, we participated in the iBeetle screen. The iBeetle screen was a large-scale RNAi screen in Tribolium, which identified functions in embryonic and postembryonic development for more than half of the Tribolium genes . Here we report on the identification of the putative CK2 substrate TC003132. We show that TC003132 crucially contributes to the specification of the follicle stem cell linage in the telotrophic Tribolium ovary.
The initial phenotype for TC003132 (iB_00521) was found and reproduced in the Pig-19  strain of Tribolium castaneum. All subsequent experiments were performed in the wild type strain San Bernadino (SB) . No differences in the phenotype due to strain-specific effects could be observed . All beetles were reared under standard conditions  on white wheat flour containing 5% dry yeast at 25 °C and shifted to 32 °C for the experiments.
Dissection and antibody staining
Dissection and fixation of adult ovaries was essentially performed as described previously [14–16] (see also Additional file 1: Methods). To visualize morphology, Hoechst 33,258 (5 μg/ml) and Phalloidin (Atto488 or Atto647N fluorophore) (1:50; Sigma) were used. The anti-β-catenin antibody (gift of Gregor Bucher) was used at a 1:500 dilution. To detect mitotically active cells, an anti-phospho-Histone H3 antibody (rabbit polyclonal, 1:100, Upstate) was used. The crossreacting mouse monoclonal anti-Drosophila-Eyes-absent and anti-Drosophila-Cut antibodies (eya10H6 and 2B10 respectively, DSHB) were used in a 1:100 dilution. The following secondary antibodies were all used at a 1:200 dilution: Alexa555-conjugated goat anti-mouse (Invitrogen), DyLight649 conjugated goat anti-rabbit (Jackson Immuno-Research).
EdU incorporation assay
For the EdU (5-ethynyl-2′-deoxyuridine) experiments we used the EdU-Click 488 kit (baseclick GmbH). EdU stock solution was dissolved in water (25 mM). For injections 5 mM dilutions were used. Detection was carried according to the manufacturers protocol. EdU positive cells were counted using the Cell Counter plugin of Fiji .
Results and discussion
Early follicle cell lineages in Tribolium
In Drosophila, follicle stem cells (FSC) undergo asymmetric division, and daughter cells are either specified as main-body or as polar/stalk precursors, which is distinguishable by relative expression levels of Castor (Cas) and Eyes absent (Eya) . Both, Drosophila Cas and Eya are expressed in FSCs, and as their siblings differentiate, cells either express more Cas and lose Eya, or vice versa. While cells with higher Cas apparently differentiate into polar or stalk cells, the cells in which Eya expression remains at high levels differentiate into main-body follicle cells.
In contrast to Eya, Cut, which in Drosophila is required for cell differentiation and tissue homeostasis [25–30], is not restricted to mitotically active cell, as we monitored low levels of Cut expression also in the somatic plug (SP) (Fig. 1, B–B″). The somatic plug consists of small post-mitotic cells and separates the tropharium from the vitellarium (Fig. 1, C) . All pre-follicular and mitotic active follicle cells show strong Cut expression. Again, Cut fades as the cells enter the endocycle. Only terminal follicle cells that separate individual oocytes retain high Cut levels (Fig. 1, B). While somatic plug cells can be distinguished from somatic follicle cells by marker gene expression (i.e. weak Cut expression and absence of Eya), early pre-follicular cell populations reveal distinct differences in morphology and polarisation status (Fig. 1, C). Lateral pre-follicular cells (LPC) are noticeably polarized, while central pre-follicular cells (CPC) do not show a distinct apical-basal polarity. Still, both pre-follicular cell populations show high level of Cut and Eya expression. Thus, irrespective of the less complex egg chamber architecture in telotrophic ovaries, we were able to identify two additional follicle cells subpopulations in Tribolium.
A putative follicle stem cell population
EdU positive cells in WT
1 ± 1
5.75 ± 2.38
7 ± 3
6.75 ± 2.38
4.75 ± 1.92
8.25 ± 0.83
13.25 ± 3.83
12 ± 4.85
4.75 ± 1.92
1.5 ± 0.87
314.75 ± 31.40
305.75 ± 90.34
178.5 ± 87.97
18.25 ± 9.81
0.5 ± 0.5
268.5 ± 37
308.75 ± 97.87
226.25 ± 59.63
9.25 ± 6.22
0 ± 0
Strikingly, pre-follicular subpopulations gain or lose the EdU signal at different rates. LPC cells start incorporating EdU already at 1dpi and reach a maximum number at 2dpi. At 7dpi, the number of EdU positive lateral pre-follicular cells drops to almost zero, resembling the overall trend for EdU incorporation. Central pre-follicular cells, however, start incorporating EdU not before 2dpi and moreover, EdU signals persist in a subset of central pre-follicular cells even beyond 7dpi. While the CPCs only account for 0.17% of all marked cells at 1 dpi, they represent over 70% at 7 dpi (Table 1, Fig. 2, Additional file 1: Table S1). Given that the EdU is used up already after 1–2 dpi, our results indicate that these label retaining CPCs represent a previously unknown subpopulation of central pre-follicular cells, dividing at slow rates.
Given that a slow division rate is one the hallmarks of stem cells and that retention of a thymidine analogue has been proven to be indicative for stem cells in various systems [33–37], it is tempting to speculate that these slow dividing cell may represent the follicle stem cell population of the telotrophic ovary.
TC003132 is required for early follicle cell specification
To identify genes that are required for the early somatic follicle cell lineage in telotrophic oogenesis, we screened the iBeetle database . The iBeetle screen was a large-scale RNAi screen Tribolium, which identified functions in embryonic and postembryonic development for more than half of the Tribolium genes . Among others, we identified TC003132 as candidate gene for early follicle cell patterning. TC003132 encodes a previously uncharacterized protein, which is widely conserved within insects, but is not found in Drosophila . Searching the NCBI Conserved Domain Database  revealed that TC003132 belongs to the Casein Kinase 2 substrate (CK2S) family (pfam15011).
Hence, our results suggest that TC003132 has (at least) a dual function. In the transition zone, it is mainly required for the specification and/or maintenance of central pre-follicular cells. In addition, TC003132 has a rather global function in promoting mitosis of follicle cells in the vitellarium.
Tc-cut RNAi results in the overproliferation of somatic follicle cell lineages
Given that that TC003132 is required to promote mitotic activity of the somatic follicle stem cell linage in Tribolium, we asked whether TC003132 function depends on Notch-signalling and/or cut action. Previously, we showed that Notch-signalling in Tribolium is among others required to maintain the mitotic cycle of somatic follicle cells . In Drosophila, the mitosis/endocycle switch is mediated by Notch-depended down-regulation of cut expression and the loss of Drosophila cut function results in premature entry of main-body follicle cells into the endocycle .
As deduced by pH 3 staining (Fig. 6c, Additional file 1: Table S2), the number of mitotic follicle cell raised to more than 50 per ovariole. Interestingly, EdU incorporation revealed that central pre-follicular cells show the highest increase of mitotic activity, as compared to wild type (Fig. 6f). The expansion of this cell population may displace the oocytes and as a consequence, oocytes become arrange side-by-side, which eventually increases the overall diameter of the ovariole (Fig. 6b–e). Irrespective of the alignment phenotype, oocytes still grow and eventually, also enter vitellogenesis (Fig. 6d, e).
In Drosophila, Cut is required for cell differentiation and tissue homeostasis in embryonic and adult tissues [25–30]. On the one hand, Cut acts post-mitotic, i.e. regulates genes involved in cell differentiation and on the other hand, Cut was shown to maintain the normal mitotic cycle in follicle cells during Drosophila oogenesis. Also in mammals, Cut has been shown to be involved in regulating cell-cycle progression in some cell types [42–45]. While we did not observed any obvious impact of Tribolium cut on cell fate decision during follicle cell patterning in telotrophic Tribolium oogenesis, we found cut to act on cell-cycle regulation in Tribolium as well. However, Tribolium cut rather prevents (or balances) mitosis, as in absence of cut follicle cells continue to proliferate. Hence, even though cut has opposing effects on the mitotsis/endocycle switch in Tribolium and Drosophila, the general function of Cut in tissue homeostasis might well be conserved.
TC003132 acts upstream of Notch-signaling
First, we asked whether Tc-Cut and TC003132 act in the same pathway or in parallel to each other. In the double knock down situation, the follicular epithelium is less disorganized and egg-chambers are aligned properly as compared to cut knockdown (Fig. 7). Also, the overall number of mitotically active cells in TC003132 and cut double RNAi drops to almost wildtype levels (Additional file 1: Table S2). Thus, the depletion of TC003132 counteracts the Tc-cut RNAi overproliferation phenotype.
Still, pre-follicular cell populations react somewhat different to the combined knock-down of Tc-cut and TC003132. In central pre-follicular cells, the TC003132 phenotype is predominant (Fig. 7c). As deduced by Eya, CPCs are reduced in number, very similar to TC003132 RNAi (Fig. 7c–d). In lateral pre-follicular cells, however, the Tc-Cut phenotype is still obvious. We observed an (albeit slight) increase of lateral follicle cells, resulting in a partially multi-layered follicular epithelium (Fig. 7, arrows in c–d). Hence, while TC003132 counteracts the Tc-cut RNAi phenotype in most somatic cells, in central pre-follicular cell, TC003132 is epistatic to Tc-cut.
In Notch RNAi, follicle cells cease the expression of Eya and switch to endocycle (Fig. 8, B) . As a consequence, oocytes are not longer encapsulated properly and terminal follicle cells are absent. In addition, oocytes enter pre-vitellogenic growth prematurely. In the combined knock-down of TC003132 and Notch, however, Eya is still expressed at high levels, indicating that simultaneous TC003132 RNAi prevents follicle cells from entering the endocycle prematurely (Fig. 8, C). Thus, with respect to mitotic to endocycle transition, TC003132 acts upstream of Notch. However, since we observed growing oocytes in the transition zone (Fig. 8, arrow in C) TC003132 does not counteract all aspects of the Notch RNAi phenotypes.
Our results indicate that TC003132 is required for mitotic activity of pre-follicular cells. Previously, it was shown that c1orf109, which encodes a human CK2 substrate is involved in cancer cell proliferation by promoting G1 phase to S phase transition . During oogenesis, G1 phase to S phase transition is required for both, mitotic and endocycle. Thus, the function of TC003132 in cell cycle control may very well be conserved. However, while central and lateral pre-follicular are (almost) equally affected upon Notch or cut depletion, only central pre-follicular cells are lost in TC003132 RNAi. Lateral pre-follicular cells remain largely unaffected upon TC003132 depletion and still express Eya.
These results indicate that TC003132 might be required for the specification or maintenance of the central pre-follicular cell linage. Given that TC003132 depleted pre-follicular cells neither react to mitosis or endocycle stimulating signals, TC003132 may be required to provide competence for differentiation cues. Interestingly, this may resemble the situation in C. elegans where CK2 is required to maintain the balance between proliferation and differentiation in the germ line . While animals mutant for kin10 – the worm homologue of the regulatory CK2β subunit – exhibit a smaller mitotic zone in the gonad, depletion in sensitized backgrounds also revealed an additional role for kin10 in promoting the transition from mitosis to meiosis . These dual functions might be carried out via different CK2 substrates.
The earliest effect of TC003132 RNAi is characterized by the loss of Eya expression in a subpopulation of central pre-follicular cells. Strikingly, these cells resemble those slow dividing cells that retain the EdU signal. Given that these cells may resemble a follicle stem cell population, it is tempting to speculate that TC003132 is required for the proliferation or maintenance of follicle stem cells in the telotrophic Tribolium ovary. In Drosophila, two actively dividing follicle stem cells (FSC) are present per ovariole and each cell produces approximately half of the follicle cells in the ovariole . Escort cells are believed to provide niche factors that anchor the FSCs at the region 2a/2b border of the germarium and promote FSC self-renewal and differentiation . Still, the number of stem cells may vary among different systems and organisms . In the Drosophila testis, for instance, 7 to 15 germline stem cells are present and somatic hub cells serve as their niche. The number of stem cell in the Mouse hair follicle bulge is even higher, as more than 50 epithelial stem cells were identified . Also multiple subtypes of niches exist. While in the Drosophila ovary only two cap cells serve as niche for the GSCs, mammalian subventricular zone (SVZ) neural stem cells are closely associated with multiple cell types, including astrocytes, neuroblasts, ependymal cells, endothelial cells and the basal-lamina (for a review see ). Our results suggest that also telotrophic ovary harbours a larger population of FSCs. Given that these putative FSCs are in direct contact to the somatic plug, we posit that this region of the telotrophic ovariole may serve as niche for the follicle stem cell population.
Still, more work will be required to identify the molecular mechanisms by which TC003132 regulates cell proliferation and/or differentiation of the follicle stem cell linage. Among others, the expression of TC003132 remains to be elucidated. Unfortunately, ovariole-in-situ hybridisation is still not working robustly. To overcome these limitations, we generated several peptide antibodies against TC003132. However, for none of these antibodies we received conclusive results. In addition it remains to be elucidated, whether TC003132 is indeed a substrate of the casein kinase 2 (CK2). Given that CK2 is associated with cell survival, cell cycle progression, differentiation, and human cancer formation, the further analysis of TC003132 may provide insights into a variety of important cellular processes, including the formation and maintenance of somatic stem cells.
We are grateful to Ralph Rübsam and Jürgen Büning for discussions on Tribolium oogenesis. We are very thankful for valuable comments from the anonymous reviewers. All authors read and approved the manuscript.
The lab of M.S. is funded through grants by the German Research Foundation (DFG: SCHO 1058 4-2).
Availability of data and materials
All data generated or analysed during this study are included in this published article and its supplementary information files.
Conceived and designed the experiments: MT, MS. Acquisition of data: MT, NS, MB, DS. Analysis and interpretation of data: MT, NS, MS. Writing the manuscript: MT, MS. All authors read and approved the final manuscript.
The authors declare that they have no competing interests.
Consent for publication
Ethics approval and consent to participate
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- Kirilly D, Xie T. The Drosophila ovary: an active stem cell community. Cell Res. 2007;17:15–25.View ArticlePubMedGoogle Scholar
- Losick VP, Morris LX, Fox DT, Spradling A. Drosophila stem cell niches: a decade of discovery suggests a unified view of stem cell regulation. Dev Cell. 2011;21:159–71.View ArticlePubMedGoogle Scholar
- Sahai-Hernandez P, Nystul TG. A dynamic population of stromal cells contributes to the follicle stem cell niche in the Drosophila ovary. Development. 2013;140:4490–8.View ArticlePubMedPubMed CentralGoogle Scholar
- Margolis J, Spradling A. Identification and behavior of epithelial stem cells in the Drosophila ovary. Development. 1995;121:3797–807.PubMedGoogle Scholar
- Song X, Xie T. DE-cadherin-mediated cell adhesion is essential for maintaining somatic stem cells in the Drosophila ovary. Proc Natl Acad Sci. 2002;99:14813–8.View ArticlePubMedPubMed CentralGoogle Scholar
- Nystul T, Spradling A. Regulation of epithelial stem cell replacement and follicle formation in the Drosophila ovary. Genetics. 2010;184:503–15.View ArticlePubMedPubMed CentralGoogle Scholar
- Nystul T, Spradling A. An epithelial niche in the Drosophila ovary undergoes long-range stem cell replacement. Cell Stem Cell. 2007;1:277–85.View ArticlePubMedGoogle Scholar
- Chang YC, Jang AC, Lin CH, Montell DJ. Castor is required for Hedgehog-dependent cell-fate specification and follicle stem cell maintenance in Drosophila oogenesis. Proc Natl Acad Sci U S A. 2013;110:E1734–42.View ArticlePubMedPubMed CentralGoogle Scholar
- Assa-Kunik E, Torres IL, Schejter ED, Johnston DS, Shilo BZ. Drosophila follicle cells are patterned by multiple levels of Notch signaling and antagonism between the Notch and JAK/STAT pathways. Development. 2007;134:1161–9.View ArticlePubMedGoogle Scholar
- Xi R, McGregor JR, Harrison DA. A gradient of JAK pathway activity patterns the anterior-posterior axis of the follicular epithelium. Dev Cell. 2003;4:167–77.View ArticlePubMedGoogle Scholar
- Johnston MJ, Bar-Cohen S, Paroush Z, Nystul TG. Phosphorylated Groucho delays differentiation in the follicle stem cell lineage by providing a molecular memory of EGFR signaling in the niche. Development. 2016;143:4631–42.View ArticlePubMedGoogle Scholar
- Lopez-Schier H, St Johnston D. Delta signaling from the germ line controls the proliferation and differentiation of the somatic follicle cells during Drosophila oogenesis. Genes Dev. 2001;15:1393–405.View ArticlePubMedGoogle Scholar
- Roth S, Lynch JA. Symmetry breaking during Drosophila oogenesis. Cold Spring Harb Perspect Biol. 2009;1:a001891.View ArticlePubMedPubMed CentralGoogle Scholar
- Trauner J, Buning J. Germ-cell cluster formation in the telotrophic meroistic ovary of Tribolium castaneum (Coleoptera, Polyphaga, Tenebrionidae) and its implication on insect phylogeny. Dev Genes Evol. 2007;217:13–27.View ArticlePubMedGoogle Scholar
- Baumer D, Strohlein NM, Schoppmeier M. Opposing effects of Notch-signaling in maintaining the proliferative state of follicle cells in the telotrophic ovary of the beetle Tribolium. Front Zool. 2012;9:15.View ArticlePubMedPubMed CentralGoogle Scholar
- Baumer D, Trauner J, Hollfelder D, Cerny A, Schoppmeier M. JAK-STAT signalling is required throughout telotrophic oogenesis and short-germ embryogenesis of the beetle Tribolium. Dev Biol. 2011;350:169–82.View ArticlePubMedGoogle Scholar
- Calvi BR, Lilly MA, Spradling AC. Cell cycle control of chorion gene amplification. Genes Dev. 1998;12:734–44.View ArticlePubMedPubMed CentralGoogle Scholar
- Deng WM, Althauser C, Ruohola-Baker H. Notch-Delta signaling induces a transition from mitotic cell cycle to endocycle in Drosophila follicle cells. Development. 2001;128:4737–46.PubMedGoogle Scholar
- Schmitt-Engel C, Schultheis D, Schwirz J, Strohlein N, Troelenberg N, Majumdar U, Dao VA, Grossmann D, Richter T, Tech M, et al. The iBeetle large-scale RNAi screen reveals gene functions for insect development and physiology. Nat Commun. 2015;6:7822.View ArticlePubMedPubMed CentralGoogle Scholar
- Lorenzen MD, Berghammer AJ, Brown SJ, Denell RE, Klingler M, Beeman RW. piggyBac-mediated germline transformation in the beetle Tribolium castaneum. Insect Mol Biol. 2003;12:433–40.View ArticlePubMedGoogle Scholar
- Sokoloff A, Slatis HM, Stanley J. The black mutation in Tribolium castaneum. J Hered. 1960;51:131–5.Google Scholar
- Kitzmann P, Schwirz J, Schmitt-Engel C, Bucher G. RNAi phenotypes are influenced by the genetic background of the injected strain. BMC Genomics. 2013;14:5.View ArticlePubMedPubMed CentralGoogle Scholar
- Brown SJ, Shippy TD, Miller S, Bolognesi R, Beeman RW, Lorenzen MD, Bucher G, Wimmer EA, Klingler M. The red flour beetle, Tribolium castaneum (Coleoptera): a model for studies of development and pest biology. Cold Spring Harb Protoc. 2009;2009:pdb-emo126.View ArticlePubMedGoogle Scholar
- Schindelin J, Arganda-Carreras I, Frise E, Kaynig V, Longair M, Pietzsch T, Preibisch S, Rueden C, Saalfeld S, Schmid B, et al. Fiji: an open-source platform for biological-image analysis. Nat Methods. 2012;9:676–82.View ArticlePubMedGoogle Scholar
- Blochlinger K, Jan LY, Jan YN. Postembryonic patterns of expression of cut, a locus regulating sensory organ identity in Drosophila. Development. 1993;117:441–50.PubMedGoogle Scholar
- Blochlinger K, Jan LY, Jan YN. Transformation of sensory organ identity by ectopic expression of Cut in Drosophila. Genes Dev. 1991;5:1124–35.View ArticlePubMedGoogle Scholar
- Jack J, DeLotto Y. Effect of wing scalloping mutations on cut expression and sense organ differentiation in the Drosophila wing margin. Genetics. 1992;131:353–63.PubMedPubMed CentralGoogle Scholar
- Jackson SM, Blochlinger K. cut interacts with Notch and protein kinase A to regulate egg chamber formation and to maintain germline cyst integrity during Drosophila oogenesis. Development. 1997;124:3663–72.PubMedGoogle Scholar
- Merritt DJ. Transformation of external sensilla to chordotonal sensilla in the cut mutant of Drosophila assessed by single-cell marking in the embryo and larva. Microsc Res Tech. 1997;39:492–505.View ArticlePubMedGoogle Scholar
- Sun J, Deng WM. Notch-dependent downregulation of the homeodomain gene cut is required for the mitotic cycle/endocycle switch and cell differentiation in Drosophila follicle cells. Development. 2005;132:4299–308.View ArticlePubMedPubMed CentralGoogle Scholar
- Buck SB, Bradford J, Gee KR, Agnew BJ, Clarke ST, Salic A. Detection of S-phase cell cycle progression using 5-ethynyl-2′-deoxyuridine incorporation with click chemistry, an alternative to using 5-bromo-2′-deoxyuridine antibodies. BioTechniques. 2008;44:927–9.View ArticlePubMedGoogle Scholar
- Salic A, Mitchison TJ. A chemical method for fast and sensitive detection of DNA synthesis in vivo. Proc Natl Acad Sci U S A. 2008;105:2415–20.View ArticlePubMedPubMed CentralGoogle Scholar
- Illa-Bochaca I, Montuenga LM. The regenerative nidi of the locust midgut as a model to study epithelial cell differentiation from stem cells. J Exp Biol. 2006;209:2215–23.View ArticlePubMedGoogle Scholar
- Karpowicz P, Morshead C, Kam A, Jervis E, Ramunas J, Cheng V, van der Kooy D. Support for the immortal strand hypothesis: neural stem cells partition DNA asymmetrically in vitro. J Cell Biol. 2005;170:721–32.View ArticlePubMedPubMed CentralGoogle Scholar
- Smith GH. Label-retaining epithelial cells in mouse mammary gland divide asymmetrically and retain their template DNA strands. Development. 2005;132:681–7.View ArticlePubMedGoogle Scholar
- Tan J, Xu M, Zhang K, Wang X, Chen S, Li T, Xiang Z, Cui H. Characterization of hemocytes proliferation in larval silkworm, Bombyx mori. J Insect Physiol. 2013;59:595–603.View ArticlePubMedGoogle Scholar
- Tumbar T, Guasch G, Greco V, Blanpain C, Lowry WE, Rendl M, Fuchs E. Defining the epithelial stem cell niche in skin. Science. 2004;303:359–63.View ArticlePubMedGoogle Scholar
- Donitz J, Schmitt-Engel C, Grossmann D, Gerischer L, Tech M, Schoppmeier M, Klingler M, Bucher G. iBeetle-Base: a database for RNAi phenotypes in the red flour beetle Tribolium castaneum. Nucleic Acids Res. 2015;43:D720–5.View ArticlePubMedGoogle Scholar
- Kriventseva EV, Tegenfeldt F, Petty TJ, Waterhouse RM, Simao FA, Pozdnyakov IA, Ioannidis P, Zdobnov EM. OrthoDB v8: update of the hierarchical catalog of orthologs and the underlying free software. Nucleic Acids Res. 2015;43:D250–6.View ArticlePubMedGoogle Scholar
- Marchler-Bauer A, Derbyshire MK, Gonzales NR, Lu S, Chitsaz F, Geer LY, Geer RC, He J, Gwadz M, Hurwitz DI, et al. CDD: NCBI’s conserved domain database. Nucleic Acids Res. 2015;43:D222–6.View ArticlePubMedGoogle Scholar
- de Miguel C, Linsler F, Casanova J, Franch-Marro X. Genetic basis for the evolution of organ morphogenesis: the case of spalt and cut in the development of insect trachea. Development. 2016;143:3615–22.View ArticlePubMedGoogle Scholar
- Coqueret O, Berube G, Nepveu A. The mammalian Cut homeodomain protein functions as a cell-cycle-dependent transcriptional repressor which downmodulates p21WAF1/CIP1/SDI1 in S phase. EMBO J. 1998;17:4680–94.View ArticlePubMedPubMed CentralGoogle Scholar
- Gupta S, Luong MX, Bleuming SA, Miele A, Luong M, Young D, Knudsen ES, Van Wijnen AJ, Stein JL, Stein GS. Tumor suppressor pRB functions as a co-repressor of the CCAAT displacement protein (CDP/cut) to regulate cell cycle controlled histone H4 transcription. J Cell Physiol. 2003;196:541–56.View ArticlePubMedGoogle Scholar
- Truscott M, Raynal L, Premdas P, Goulet B, Leduy L, Berube G, Nepveu A. CDP/Cux stimulates transcription from the DNA polymerase alpha gene promoter. Mol Cell Biol. 2003;23:3013–28.View ArticlePubMedPubMed CentralGoogle Scholar
- Wu F, Lee AS. CDP and AP-2 mediated repression mechanism of the replication-dependent hamster histone H3.2 promoter. J Cell Biochem. 2002;84:699–707.View ArticlePubMedGoogle Scholar
- S-s L, Zheng H-x, H-d J, He J, Yu Y, Y-p Q, Yue L, Zhang Y, Li Y. Identification and characterization of a novel gene, c1orf109, encoding a CK2 substrate that is involved in cancer cell proliferation. J Biomed Sci. 2012;19:49.View ArticleGoogle Scholar
- Wang X, Gupta P, Fairbanks J, Hansen D. Protein kinase CK2 both promotes robust proliferation and inhibits the proliferative fate in the C. elegans germ line. Dev Biol. 2014;392:26–41.View ArticlePubMedGoogle Scholar
- Sahai-Hernandez P, Castanieto A, Nystul TG. Drosophila models of epithelial stem cells and their niches. Wiley Interdiscip Rev Dev Biol. 2012;1:447–57.View ArticlePubMedPubMed CentralGoogle Scholar
- Ohlstein B, Kai T, Decotto E, Spradling A. The stem cell niche: theme and variations. Curr Opin Cell Biol. 2004;16:693–9.View ArticlePubMedGoogle Scholar
- Braun KM, Niemann C, Jensen UB, Sundberg JP, Silva-Vargas V, Watt FM. Manipulation of stem cell proliferation and lineage commitment: visualisation of label-retaining cells in wholemounts of mouse epidermis. Development. 2003;130:5241–55.View ArticlePubMedGoogle Scholar
- Lin R, Iacovitti L. Classic and novel stem cell niches in brain homeostasis and repair. Brain Res. 2015;1628:327–42.View ArticlePubMedGoogle Scholar