Fig. 3

(A–A′) Wild type ovariole of Tribolium stained with Phalloidin for f-Actin (red) and Hoechst (blue). In wild type, pro-oocytes leave the tropharium, become encapsulated by follicle cells and are aligned in the centre (A′, magnification of indicated region in A). (B–B′) Pupal knockdown of TC003132 at 7dpi. The vitellarium is shortened and only a single growing follicle is obvious (B). Oocytes are not longer encapsulated, resulting in oocytes arranged side-by-side (B′). Asterisks mark oocytes and Arrows point to oocytes contacting each other, thus indicating encapsulation defects. Scale bar in A and B: 50 μm