Fig. 8

(A–A′) Wild type ovariole stained with Phalloidin to visualize f-Actin (red) and an antibody against Eya (green). Mitotic active follicle cells strongly express Eya, while cells that entered the endocycle cease Eya expression. (A′) Magnification of the indicated region in (A). (B) Knockdown of Notch results in the premature entry of the follicle cells into the endocycle. (C) Double RNAi of Notch and TC003132 suppresses the effects of Notch RNAi in follicle cells: Eya expression is restored. Still, the germline shows signs of the Notch phenotype as prematurely growing oocytes are still obvious (C, arrow). Solid green and light green bars below ovarioles indicate regions of strong and weak Eya expression, respectively. Scale bar: 10 μm