Fig. 5

a–b Staining of phosphorylated histon H3 (PH3, green) and Phalloidin (red) in wild type and TC003132 RNAi ovarioles. c Box plot of PH3 positive cells in WT (green) and TC003132 RNAi at 5dpi (red). Centre lines show the medians; box limits indicate the 25th and 75th percentiles as determined by R software; whiskers extend 1.5 times the interquartile range from the 25th and 75th percentiles. n = 13, 14 sample points. d–f Detection of EdU (green) and ß-Catenin (red) in wild type and TC003132 RNAi ovarioles. Compared to wild type, the amount of follicle cells in mitosis is approximately halved 5dpi after TC003132 RNAi (a–c). Both, wild type and TC003132 knockdown exhibit similar amounts of EdU incorporation at 3dpi (f). In wild type, the signal is rapidly lost in (d, f) and only the anteriormost central pre-follicular cells remain EdU positive (d). In TC003132 RNAi, the EdU signal is stable and diminishes at a much slower rate (e, f). Error bars in (f) represent standard deviation. Dpi: days post injection; NS: Not significant; ** and **** P < 0.01 and P < 0.0001 using a two-tailed t-test, respectively