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Low levels of nestmate discrimination despite high genetic differentiation in the invasive pharaoh ant

Abstract

Background

Ants typically distinguish nestmates from non-nestmates based on the perception of colony-specific chemicals, particularly cuticular hydrocarbons present on the surface of the ants' exoskeleton. These recognition cues are believed to play an important role in the formation of vast so-called supercolonies that have been described for some invasive ant species, but general conclusions about the role of these cues are hampered by only few species being studied. Here we use data on cuticular hydrocarbons, aggression and microsatellite genetic markers to investigate the interdependence of chemical recognition cues, genetic distance and nestmate discrimination in the pharaoh ant (Monomorium pharaonis), a widespread pest species, and ask whether introduced populations of this species are genetically differentiated and exhibit intraspecific aggression.

Results

Microsatellite analyses of a total of 35 colonies from four continents revealed extremely high levels of genetic differentiation between almost all colonies (FST = 0.751 ± 0.006 SE) and very low within-colony diversity. This implies that at least 34 and likely hundreds more independent lineages of this ant have spread worldwide. Aggression tests involving workers from 14 different colonies showed only low levels of aggression, even between colonies that were geographically and/or genetically very distant. Chemical analyses of groups of worker ants showed that all colonies had the same cuticular compounds, which varied only quantitatively among colonies. There was a positive correlation between geographical and genetic distance, but no other significant relationships were detected between aggression, chemical profile, genetic distance and geographical distance.

Conclusions

The pharaoh ant has a global invasion history of numerous independent introductions resulting in genetically highly differentiated colonies typically displaying surprisingly low levels of intraspecific aggression, a behaviour that may have evolved in the native range or by lineage selection in the introduced range.

Background

Unicoloniality, in which individuals from geographically separated nests intermix without aggression, has long been considered a key characteristic of invasive ants [e.g. [1–3]]. Colonies of invasive ant species can become dominant in the invaded habitats and are often termed "supercolonies" due to their sizes and huge potential for growth. Unicolonial species are assumed to have a competitive edge over other ant species, as lack of intraspecific aggression may enable a more effective resource allocation [4–7]. The precise mechanisms enabling the formation of supercolonies are a topic of some discussion [3, 8]. However, several hypotheses stress the potentially important role of diversity of recognition cues and discrimination behaviour, although the hypotheses differ with respect to the role of ecological conditions and whether important traits for supercolony formation developed before or after the ants were introduced outside their native range and became invasive pests [9–11].

Cuticular hydrocarbons (CHCs) play a central role in ant communication and nestmate discrimination [12, 13]. These lipids lower the risk of desiccation, can reflect environmental as well as genetic variation [13, 14], and likely have evolved to function as recognisable labels enabling discrimination of nestmates from non-nestmates. Several studies have established a role for CHCs in intraspecific discrimination in ants [e.g. [15–17]], and differences in cuticular hydrocarbon profiles can often be correlated with aggression probabilities, as antagonism is expected to be expressed when odour dissimilarity exceeds a given threshold [12, 18–20].

The pharaoh ant (Monomorium pharaonis) is a ubiquitous invasive ant species [21]. The species is mainly found in buildings and as such a common household pest. Like other invasive ant species it has a high potential for spread [2, 22, 23] and is polygynous, i.e. several queens reproduce in a colony. Polygyny is hypothesized to lead to lower levels of nestmate recognition relative to monogynous (single queen) species, perhaps because of the expected higher levels of genetic diversity within a colony containing multiple reproducing individuals [24]. Low levels of intraspecific aggression, if aggression depends on nestmate recognition, may therefore have facilitated the spread of pharaoh ants [2, 21, 25].

Here we test the hypothesis that pharaoh ants, like several other well-known invasive ant species, exhibit low levels of intraspecific aggression, and we investigate the possible role of cuticular hydrocarbons in discrimination behaviour by measuring aggression within and between laboratory strains and free-living colonies to (1) assess whether discrimination behaviour is common and (2) test whether it is primarily genetically or environmentally determined. In order to do so, we examined the extent to which the discrimination behaviour is correlated with the cuticular hydrocarbon profiles of the ants and the level of genetic differentiation between them. Finally, we studied discrimination abilities of M. pharaonis towards another tramp ant species, M. destructor, to (1) investigate whether aggression against other ant species occurred and (2) compare the chemical profiles of the two species to obtain chemotaxonomic evidence for the species identifications, (cuticular hydrocarbons are usually species specific [see e.g. [13, 14]]).

We found very high levels of genetic differentiation between the different colonies and discrimination behaviour to be uncommon at first, although aggression increased with time spent in the laboratory. We found no clear correlates of inter-colonial aggression. Interspecific aggression was very high, and the two species studied had clearly distinct chemical profiles.

Methods

Study material

A total of 35 colony samples of M. pharaonis were obtained in 2004; 14 of these were live samples, either from laboratory stocks (n = 6), or from field collection (primarily in or on buildings, n = 8; Table 1). Sampling was opportunistic to represent as many different localities around the world as possible. The most extensive sampling was performed in Ghana in October 2004 to obtain colonies or colony samples from the tropics, where the species is generally more common [26]. All laboratory colonies had been reared in captivity for several years. Three samples of M. destructor were also collected in the field in Ghana in October 2004 (at Cape coast, Nsawam and Winniba) to be used for interspecific behavioural experiments and chemical analyses. M. destructor is a widespread tramp ant species, very similar to M. pharaonis in habitat usage as well as social structure, and the two species would typically be found at the same type of localities although not co-occurring [[27], AMS personal observation].

Table 1 Samples of Monomorium pharaonis.

Worker samples for genetic studies were stored in 96% EtOH until analysed. Live colonies (including queens and brood) and colony samples consisting of workers to be used for behavioural and chemical studies were kept in the laboratory under uniform conditions of 25°C, 12/12 hour light/dark cycle and provided with water ad libitum and a diet of boiled egg yolk, cooked liver, honey, almonds and dead insects twice a week. Five viable colonies were kept for more than one year so that two independent series of behavioural and chemical tests (2004 and 2005) could be done. Since the workers' lifespan is 9-10 weeks on average [28] and unlikely to exceed four months (AMS, personal observation), and even the queens have rarely been observed to live for more than one year, repeating experiments after one year ensured that individuals tested in the second round would likely not carry any cues derived from their previous environments. Of the five colonies tested again in 2005, four were originally laboratory colonies and one was a field colony, collected in Ghana in 2004 (cf. Table 1).

Genetic analyses

The genetic variation and differentiation of the colonies was examined based on four microsatellite loci for which primers were developed for M. pharaonis (Table 2). DNA was extracted from 30 workers from each colony (except 6 workers from U19) by each specimen being crushed in 200 μl 5% Chelex solution. The primers were run in separate PCR reactions in 20 μl reaction volumes with 1 μl extract as template, reaction buffer, 25 mM MgCl2, 0.5 mM GATC, 10 μM forward and reverse primers and 0.1 μl Taq gold polymerase. The PCR reaction conditions consisted of an initial denaturing step of 95°C for 10 min, followed by 27 cycles of 95°C for 30 s, 60°C for 30 s, and 72°C for 30 s, and finally an extension step at 72°C for 1 h. The PCR products were run on 2% ethidium bromide agarose gels, and subsequently run on 5% polyacrylamide gels using an ABI PRISM® 377 DNA automated sequencer with Rox500 as internal standard. The four loci can be multiplexed on the ABI. GENESCAN 3.1 and GENOTYPER 3.1 were used to score the alleles.

Table 2 Microsatellite loci developed and analysed in this study

Cuticular hydrocarbon analyses

Groups of 5 workers per colony were frozen at -20°C after which surface chemicals were extracted by washing in 20 μl pentane for 10 min. Three μl of this solution was injected into an Agilent Technologies 6890N Gas Chromatograph (GC) equipped with a capillary column (HP5MS 30 m × 250 μl × 0.25 μm). The injector was a split-splitless type with helium carrying gas at 1 ml/min. The initial temperature was 70°C, and was increased at 20°C/min to 280°C, then to 310°C at 2°C/min, and held for 5 min. The GC was coupled with a 5375 Agilent Technologies Mass Spectrometer, using 70 eV electron impact ionization. Compounds were identified on the basis of their mass spectra, and comparison with standards and published spectra. One sample was run for each colony. A single, concentrated reference of 25 pooled individuals (colony U1 used) was analysed to ascertain the identity of the peaks used in the subsequent analyses.

Behavioural experiments

Standardized aggression tests were performed where dyadic encounters were continuously observed over 10 min following Giraud et al. [4] with the modification that we allowed the ants to habituate separately for 1 min within the arena before the start of the observation period. All interactions were scored in easily distinguishable categories on a scale from 0 to 5: levels 0 (no response) and 1 (antennation) were considered neutral, non-aggressive behaviours, and levels 2 (escape), 3 (gaster raising), 4 (biting), and 5 (fighting, which involved prolonged biting, often ensuing in one ant biting onto the other for the remainder of the observation period) were considered antagonistic. Within 6 weeks from field collection in 2004 a mean of 3.9 tests (median = 5, range 1-6) were carried out for each intraspecific M. pharaonis colony pair as well as for the controls (i.e. within-colony controls). 5 tests were conducted for each interspecific combination of M. pharaonis vs. M. destructor (except Gh7 with one test in two of the combinations) and for the M. destructor within-colony control tests, to obtain a matrix of 635 tests in total. In 2005 a sub-sample of 5 of the M. pharaonis colonies was tested, with 6 tests for each possible intraspecific combination of colonies as well as for the within-colony controls, resulting in 90 intraspecific tests in total. The tests were not done blind as regards the colony origin of the ants, but the interaction categories were clearly distinguishable, thus preventing any subjectivity in the interpretation of the behaviour. Moreover, the experimenter had no prior knowledge of the genetics or chemical profiles of the colonies. No ants were used for more than one test to avoid any possible effects of familiarization.

Statistical analyses

The genetic differentiation (FST) between the colonies as well as measures of allelic richness (k') and inbreeding at the colony level (FIS) were calculated using FSTAT 2.9.3.2 [29] with significance testing based on 15,000 randomisations. Standard errors were estimated by jack-knifing over colonies. As the value of FST is affected by the allelic diversity at the marker loci applied, we further calculated the standardised F'ST[30] and the estimator Dest[31] as alternative quantifications of the genetic differentiation, making comparisons with studies based on other marker loci possible [32].

The program M_ratio[33], was applied to test for signs of population bottlenecks as M is the ratio of allele number to range in allele sizes (M = k/r ), and a significant reduction in M relative to the expected distribution is interpreted as an indication of a recent reduction in population size. The estimates of M were based on simulation of 10,000 replicates for the polymorphic loci. We applied the non-spatial genetic mixture analysis implemented in BAPS 5.3 [34] to cluster colonies that are likely to be genetically similar e.g. from having a common population history or representing the same supercolony [35, 36]. The maximum number of genetically divergent groups (K) was set to the number of colonies included in the data set, and each analysis was repeated 10 times to ensure consistency of results between different runs. The genetically divergent groups of colonies identified this way are interpreted as colony lineages. Based on the observed number of lineages in our sample we estimated the total number of such lineages in the world by the Chao1 estimator of Chao [37] using the program SPADE [38]. Furthermore, a principal component analysis (PCA) of the microsatellite allele frequency data was performed in PCAGEN 1.3.1 [39], as an exploratory technique to visualise colony or population associations possibly reflecting the phylogeographic history of the species.

For the data on chemical profiles Statistica 7 (StatSoft) was used to perform PCA on peak areas, after log-ratio transformation according to Aitchison [40]. In addition, principal components combined explaining a minimum of 95% of the variation were found in JMP.IN 5.1 and subsequently used for calculating the inter-sample chemical (Euclidian) distances as a measure of their relative differences. Cluster analyses were performed based on the cuticular hydrocarbon data, see additional file 1.

Mantel tests for possible correlations between the chemical and behavioural, genetic as well as geographical distances were performed in FSTAT [29], all based on 10,000 permutations. The results of the subset of colonies that were studied in both 2004 and 2005 were compared with MANOVA. The behavioural data were analysed using Kruskal-Wallis test with Dunn's post-hoc test and Mann-Whitney U-test for the 2004 and 2005 data respectively; Fisher's exact test was applied to compare the overall occurrence of aggression between the two years.

Results

Genetic diversity and differentiation

Table 2 gives information on the four microsatellite markers that were developed for this study. At one locus (Mp12) only two alleles were found, and all colonies except one (TW) were monomorphic for one of these alleles, while TW was monomorphic for the other. The genetic diversity within colonies was generally very low, as over 2/3 of the colonies only had two polymorphic loci, and five of these colonies were monomorphic at all four loci (Table 1). The allelic richness (k') was 1.47 ± 0.07 SE, the expected heterozygosity (Hexp) was 0.153 ± 0.023 SE on average across colonies and, as expected, these measures of the genetic diversity were highly correlated (Spearman r = 0.977; P < 0.0001). The calculations from the M_ratio program indicated that 14 of the colonies had likely gone through a reduction in the effective number of reproducing individuals (Table 1).

There were no signs of deviation from random mating within the colony samples (FIS = -0.021 ± 0.005 SE; P = 0.82), confirming that each colony formed its own breeding population as seen in other invasive ant species (e.g. [35]). We consistently found very high and statistically significant values of interpopulation genetic differentiation (mean FST = 0.751 ± 0.006 SE; P < 0.0001; corresponding to F'ST = 0.891 and Dest = 0.561; see also additional file 2 for estimates of pairwise FST). Only two colonies (U1 from Florida and U19 from Panama) were grouped as genetically similar by the BAPS analysis, as also reflected in the PCA plot (Fig. 1), and this result (K = 34) had strong support by a posterior probability P = 1.000 in all 10 runs. Consequently, the group of these two colonies and the 33 remaining colonies were all significantly genetically divergent, indicating that there were 34 independent lineages represented in our sample. Extrapolating this finding using the Chao estimator implies that the total number of such lineages of pharaoh ants in the world should be counted in hundreds, maybe even thousands (SChao1 = 579; 140-3005 95% C.I.).

Figure 1
figure 1

Principal Component Analysis of microsatellite allele frequencies of Monomorium pharaonis colonies. Colour coding is by continent; red: Africa, green: Europe, blue: America, purple: Asia. Colony labels are as in Table 1. The explained variances are given in parentheses.

Cuticular hydrocarbon analyses

The cuticular profile of M. pharaonis was characterized by 18 different hydrocarbons, which were a mix of unbranched, mono- and di-methyl-branched alkanes with 25 to 33 carbons and unsaturated hydrocarbons (Fig. 2a). As these hydrocarbons were invariably present, our analysis focused on quantitative differences among M. pharaonis colonies. For the 2004 and 2005 data a combined PCA extracted 5 principal components with eigenvalues larger than 1 (eigenvalues and percent variance explained by each of the 5 components: 5.83, 32%; 4.53, 25%; 2.22, 12%; 1.52, 8%; 1.10, 6%). PCA of all cuticular profiles did not provide clear groupings in either year (Fig. 3), and there were no significant differences in inter-colonial chemical distances between the years (MANOVA on first four principal components, F3,2 = 0.57; P = 0.68), indicating that laboratory rearing had not lead to convergence of the cuticular profiles. From Fig. 3 there seem to be changes between years, but these are not consistently in any direction, although our sample size of five colonies is too small to test this statistically or to test directly for significant colony-specific changes between years.

Figure 2
figure 2

Cuticular hydrocarbon profiles of Monomorium pharaonis (a) and M. destructor (b). The numbers on the peaks mark the identified hydrocarbons: (1*) C25:1; (2*) C25; (3*) 9-,11-,13-MeC25; (4) C26; (5*) C27:1; (6*) C27; (7) 9-,11-,13-MeC27; (8*) 11,15-diMeC27; (9) 3-MeC27; (10*) 3,11-diMeC27; (11) C29:1; (12*) C29; (13) 11-,13-,15-MeC29; (14) 11,15-diMeC29; (15) 3,11-diMeC29; (16*) C31:1; (17*) C31; (18) 11-,13-,15-MeC31; (19) C28; (20) 5-MeC29; (21) C30; (22) 11-,13-MeC31; (23) 7-MeC31; (24) 5-MeC31; (25) 11-,13-MeC33; (26) 7-MeC33. The numbers with an asterisk denote the hydrocarbons that explain most of the variation in the overall principal component analysis, i.e. numerical value of factor loadings > 0.7. Factor loadings for these compounds were: (1) -0.736; (2) -0.835; (3) -0.867; (5) -0.745; (6) -0.885; (8) -0.768; (10) 0.797; (12) -0.751; (16) 0.902; (17) -0.841.

Figure 3
figure 3

Principal Component Analysis of cuticular hydrocarbons of Monomorium pharaonis colonies. Filled and open symbols indicate colonies analysed in 2004 (n = 14) and 2005 (n = 5), respectively. Colonies from Ghana are shown by triangles, whereas the rest are shown by circles. Dashed lines are drawn between the symbols representing colonies analysed in 2004 and 2005. Colony labels are as in Table 1. The explained variances are given in parentheses.

Ten hydrocarbons accounted for most intercolony variation (i.e. factor loadings > 0.7) and were a mix of different types, branched as well as unbranched (see legend of Fig. 2a). For the 2004 data there was no significant correlation between chemical distances and level of aggression across colonies (Mantel test, n = 14, r = 0.047; P = 0.65; Fig. 4). For further illustration of the seeming lack of geographical clustering based on the chemical data see Ward tree based on hierarchical cluster analysis in additional file 1.

Figure 4
figure 4

Association of genetic, chemical, behavioural, and geographical distances between colonies of Monomorium pharaonis. Correlation coefficients as well as partial correlation coefficients are given for each plot. Mantel tests yielded insignificant correlations (P > 0.09), except for genetic vs. geographical distance (P = 0.005).

When compared with M. destructor, the M. pharaonis chemical profiles were clearly qualitatively different: M. destructor consisted of 12 hydrocarbons of which only four were also found in M. pharaonis (Fig. 2).

Aggression assays

In 45 behavioural tests no interactions between the ants were observed, leaving 590 tests for further analyses. Aggression occurred in 23% (21 out of 91 combinations) of the intraspecific colony pairs obtained in 2004. The average aggression level was 0.99 ± 0.05 SD for 14 within-colony control pairs (median = 1, interquartile range = 0), 1.37 ± 0.82 for 91 intraspecific pairs (median = 1, interquartile range = 0.29), and 3.68 ± 0.98 for 42 interspecific pairs (median = 3.8, interquartile range = 1.55). There was a significant overall difference (Kruskal-Wallis test, n = 147, H = 85; P < 0.0001), the difference between intra- and interspecific aggression also being significant (Dunn's post-hoc test, n = 2; both P < 0.001), but no significant difference was found between intraspecific and within-colony (control) aggression (Dunn's post-hoc test, n = 2; P > 0.05), reflecting a generally rare occurrence of intraspecific aggression. The level of aggression between different colonies of M. pharaonis did not depend on the presence of queens (Kruskal-Wallis test on queenright-queenright, queenright-queenless and queenless-queenless colony pairs, n = 91, H = 2.96; P = 0.23). The second series showed higher overall occurrence of intraspecific aggression after one year: whereas aggression was observed in only 1 of the 10 colony combinations in 2004, it was observed in 7 of the 10 combinations in 2005 (Fisher's exact test; P = 0.02) and there was a significant difference between intraspecific and within-colony (control) aggression in 2005 (Mann-Whitney U-test, n = 15, U = 5; P = 0.019).

Correlation analyses

The Mantel tests for association of geographical, genetic, chemical, and behavioural distances between colonies (n = 14) only showed a significant positive correlation for the combination of genetic and geographical distance (Fig.4; r = 0.30, P = 0.005; see Table 1 for colony IDs). Tests for correlation between genetic and geographical distances were also performed for the entire sample of 35 M. pharaonis colonies, as well as for the sub-set from Ghana. The correlation between genetic and geographical distance was also significant for the 35 colonies (r = 0.25, P = 0.0001). This indicates that there might be genetic isolation by distance between colonies at the global scale. However, the same correlation was not found when the sub-set of colonies from Ghana was analysed separately (n = 14, r = 0.06; P = 0.57). Thus this apparent isolation by distance appears to be a product of generally very high levels of genetic differentiation between the colonies, combined with slightly lower levels of differentiation found among the African samples, which, due to their high representation (16 of 35 colonies), skew the distribution in the shorter distance end of the geographical scale.

Discussion

The very high genetic differentiation between almost all colonies studied suggests that pharaoh ants have made their way to at least four different continents through numerous human-mediated introductions resulting in at least 34 and likely hundreds more independent lineages. Colonies appear to have frequently gone through genetic bottlenecks in the introduction process, resulting in possibly isolated colonies with low genetic diversity and extremely high intercolonial differentiation. In fact, to our knowledge, the FST value found (0.751) is the largest estimate for genetic differentiation at nuclear loci reported for any non-selfing sexually-reproducing organism. The extreme genetic divergence among lineages is not a statistical artefact of analysing markers of limited allelic diversity, as also the unbiased estimators F'ST and Dest are exceeded by only one similar species among the studies reviewed by Heller and Siegismund [32]. Hence, the pattern of dispersal and organisation in independent supercolonies closely resembles that of other invasive ant species like the Argentine ant (Linepithema humile), FST = 0.419; [36] and the invasive garden ant (Lasius neglectus), FST = 0.334; [41], but is characterised by an even larger degree of isolation, likely reflecting a longer invasion history.

We successfully identified 26 cuticular hydrocarbons from the profiles of M. pharaonis and M. destructor and found only quantitative variation between M. pharaonis colonies but clear qualitative differences between the two species (Fig. 2). Although there were extremely high levels of genetic differentiation between the M. pharaonis colonies, which led us to speculate whether several cryptic species might be involved, the cuticular data provide chemical evidence that this is not the case. The PCA of the cuticular profiles (Fig. 3) did not disclose any geographical or other groupings among the apparently chemically very similar M. pharaonis colonies investigated. Previous studies on the little fire ant (Wasmannia auropunctata) and the Argentine ant (Li. humile) have shown quantitative as well as qualitative differences in chemical profiles between native as well as introduced colonies, with introduced colonies generally having fewer compounds in their profiles [42, 43]. These findings indicate that CHC analyses could potentially be an indirect way of discerning between ants from the native and introduced ranges. However, in our sample all pharaoh ants analysed were chemically qualitatively similar, and had relatively few compounds in their profiles relative to other ant species. This indicates that they are either all from introduced colonies, or that the pattern found in W. auropunctata and Li. humile does not apply to this species.

Recent investigations suggest that double-bonded and methylated hydrocarbons may be more informative for nestmate discrimination than other hydrocarbons and that a combination of different classes of hydrocarbons may be necessary for efficient discrimination [17, 44–47]. The presence of several methylated hydrocarbons in M. pharaonis cuticular profiles therefore indicates that information for nestmate recognition is potentially available.

For another invasive ant species, the Argentine ant, Suarez et al. [48] found a correlation between cuticular chemistry and behaviour even after a year of laboratory rearing, suggesting that nestmate discrimination is a direct function of chemical distance. Likewise, Liang and Silverman [16] and Liang et al., [49] found strong correlational evidence for the importance of environmentally derived hydrocarbon cues in the discrimination behaviour of Argentine ants. The lack of correlation found in our study indicates that there is no similarly simple role of cuticular hydrocarbons in M. pharaonis. Interestingly, between-colony aggression had increased after one year of laboratory rearing, the precise mechanism of this, however, is yet to be studied. Like other invasive ants [3, 6], M. pharaonis was less aggressive against conspecifics than would be expected based merely on the large geographical distances. For example, there is >8000 km between the completely non-aggressive colonies Gh1 and U1, which are highly genetically distinct (FST = 0.824; Bonferroni corrected P < 0.05). Although queenless workers are often found to be less aggressive than queenright workers [50], this is not the case for many species including two other invasive ant species, the Argentine ant and the fire ant [51, 52]), giving us good reason to assume that this would also not be the case for pharaoh ants. As our results show no difference in the average level of aggression in interactions involving queenright and/or queenless colonies, we believe that using workers from queenless colonies has not affected our results.

The absence of aggression between distantly related colonies is remarkable and may be a characteristic of the pharaoh ant as an invasive species. As has been suggested for La. neglectus[11] and La. austriacus[53], a reduced diversity of recognition cues or a general abandoning of aggression towards conspecifics can be possible pre-adaptations in the native range for the later formation of invasive supercolonies. Alternatively, selection for increased acceptance may have taken place as lineage selection among the large number of isolated colonies in the introduced area [8, 54], with the likely benefit of having fewer false rejections of colony members in a changed ecological setting where different lineages will rarely compete directly [55, 56]. One could question whether low levels of intraspecific aggression would provide species like the pharaoh ant with a competitive edge relative to other ant species as it seems likely that they do not necessarily face very intense interspecific competition in their introduced range. This is because they primarily - possibly exclusively - occupy habitats that are characterized by human disturbance and therefore contain few important native competitors. Studying the species' distribution patterns on a more local scale may provide useful knowledge to evaluate which selective pressures they are exposed to, and whether low levels of intraspecific aggression potentially enabling the merger of introduced colonies may be advantageous. As successful ant invaders appear well adapted to survival in disturbed environments, the role of disturbance in ant invasions [57, 58] as well as in the evolution of unicoloniality [59, 60] is currently debated.

Absence of detectable aggression or intraspecific discrimination behaviour as measured in tests such as those applied in this study does not necessarily imply that the ants are not able to tell nestmates from non-nestmates, only that they do not react on perceived differences by exhibiting aggression [see also e.g. [53, 60]]. The exact mechanism of the low level of aggression among pharaoh ants is not clear from the present study, and may therefore not reflect a lack of information, but rather an impairment in the perception of variation, a modified acceptance threshold in the colonies tested [9, 61], or a lack of response to perceived cues [53]. Although we cannot rule out that other substances, such as glandular secretions, may affect nestmate discrimination in pharaoh ants, the possibility that a neurological perception mechanism to ignore kinship information may have been convergently selected in invasive ants could provide an exciting area for further study.

Conclusions

We found that the investigated colonies of pharaoh ants were very highly genetically differentiated, but did not exhibit correspondingly high levels of intraspecific aggression or divergence in cuticular hydrocarbon profiles. Uniform rearing in the laboratory appeared to increase the observed level of aggression, which, if supported by additional studies, could be seen as indirect evidence for genetically based nestmate discrimination behaviour that is otherwise not expressed in more diverse environments.

No colonies stood out in terms of genetic or chemical composition, and it is therefore not possible to draw any conclusions regarding the geographical origin of the species. Further research is needed to elucidate the population genetics of the species on a finer geographical scale as well as the role of low levels of aggression towards conspecifics in the species' invasive abilities. As pharaoh ants can be kept in the laboratory and colonies crossed, it lends much promise for further investigations on these questions.

References

  1. Wilson EO: The Insect Societies. 1971, Cambridge, Massachusetts: Harvard University Press

    Google Scholar 

  2. Passera L: Characteristics of tramp species. Exotic Ants, Biology, Impact, and Control of Introduced Species. Edited by: Williams DF. 1994, Boulder Colorado: Westview Press, 23-43. (Breed MD (series Editor): Westview Studies in Insect Biology

    Google Scholar 

  3. Holway DA, Lach L, Suarez AV, Tsutsui ND, Case TJ: The causes and consequences of ant invasions. Annu Rev Ecol Syst. 2002, 33: 181-233. 10.1146/annurev.ecolsys.33.010802.150444.

    Article  Google Scholar 

  4. Giraud T, Pedersen JS, Keller L: Evolution of supercolonies: The Argentine ants of southern Europe. Proc Natl Acad Sci USA. 2002, 99: 6075-6079. 10.1073/pnas.092694199.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  5. Suarez AV, Holway DA, Tsutsui ND: Genetics and behavior of a colonizing species: The invasive argentine ant. Am Nat. 2008, 172: S72-S84. 10.1086/588638.

    Article  PubMed  Google Scholar 

  6. Tsutsui ND, Suarez AV: The colony structure and population biology of invasive ants. Conserv Biol. 2003, 17: 48-58. 10.1046/j.1523-1739.2003.02018.x.

    Article  Google Scholar 

  7. Tsutsui ND, Suarez AV, Holway DA, Case TJ: Reduced genetic variation and the success of an invasive species. Proc Natl Acad Sci USA. 2000, 97: 5948-5953. 10.1073/pnas.100110397.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  8. Helantera H, Strassmann JE, Carrillo J, Queller DC: Unicolonial ants: where do they come from, what are they and where are they going?. Trends Ecol Evol. 2009, 24: 341-349. 10.1016/j.tree.2009.01.013.

    Article  PubMed  Google Scholar 

  9. Starks PT: Selection for uniformity: xenophobia and invasion success. Trends Ecol Evol. 2003, 18: 159-162. 10.1016/S0169-5347(03)00038-7.

    Article  Google Scholar 

  10. Jackson DE: Social Evolution: Pathways to Ant Unicoloniality. Curr Biol. 2007, 17: R1063-R1064. 10.1016/j.cub.2007.10.031.

    Article  CAS  PubMed  Google Scholar 

  11. Cremer S, Ugelvig LV, Drijfhout FP, Schlick-Steiner BC, Steiner FM, Seifert B, Hughes DP, Schulz A, Petersen KS, Konrad H: The Evolution of Invasiveness in Garden Ants. PLoS ONE. 2008, 3: e3838-10.1371/journal.pone.0003838.

    Article  PubMed Central  PubMed  Google Scholar 

  12. Lenoir A, Fresneau D, Errard C, Hefetz A: Individuality and colonial identity in ants: the emergence of the social representation concept. Information processing in social insects. Edited by: Detrain C, Deneubourg JL, Pasteels JM. 1999, Basel: Birkhäuser, 219-237. 1

    Chapter  Google Scholar 

  13. d'Ettorre P, Lenoir A: Nestmate Recognition. Ant Ecology. Edited by: Lach L, Parr CL, Abbott KL. 2010, New York: Oxford University Press, 197-209.

    Google Scholar 

  14. Vander Meer RK, Breed MD, Winston ML, Espilie KE, Eds: Pheromone Communication in Social Insects: Ants, Wasps, Bees, and Termites. 1998, Boulder Colorado: Westview Press

  15. Lahav S, Soroker V, Hefetz A, Vander Meer RK: Direct behavioral evidence for hydrocarbons as ant recognition discriminators. Naturwissenschaften. 1999, 86: 246-249. 10.1007/s001140050609.

    Article  CAS  Google Scholar 

  16. Liang D, Silverman J: "You are what you eat": Diet modifies cuticular hydrocarbons and nestmate recognition in the Argentine ant, Linepithema humile. Naturwissenschaften. 2000, 87: 412-416. 10.1007/s001140050752.

    Article  CAS  PubMed  Google Scholar 

  17. Guerrieri FJ, Nehring V, Jørgensen CG, Nielsen J, Galizia CG, d'Ettorre P: Ants recognize foes and not friends. Proc R Soc B:Biol Sci. 2009, 276: 2461-2468. 10.1098/rspb.2008.1860.

    Article  CAS  Google Scholar 

  18. Jutsum AR, Saunders TS, Cherrett JM: Intraspecific aggression in the leaf-cutting ant Acromyrmex octospinosus. Anim Behav. 1979, 27: 839-844. 10.1016/0003-3472(79)90021-6.

    Article  Google Scholar 

  19. Buczkowski G, Silverman J: Geographical variation in Argentine ant aggression behaviour mediated by environmentally derived nestmate recognition cues. Anim Behav. 2006, 71: 327-335. 10.1016/j.anbehav.2005.04.012.

    Article  Google Scholar 

  20. Dalecky A, Renucci M, Tirard A, Debout G, Roux M, Kjellberg F, Provost E: Changes in composition of cuticular biochemicals of the facultatively polygynous ant Petalomyrmex phylax during range expansion in Cameroon with respect to social, spatial and genetic variation. Mol Ecol. 2007, 16: 3778-3791. 10.1111/j.1365-294X.2007.03429.x.

    Article  PubMed  Google Scholar 

  21. Berndt KP, Eichler W: Die Pharaoameise, Monomorium pharaonis (L.) (Hym., Myrmicidae). Mitt Zool Mus Berl. 1987, 63: 3-186. 10.1002/mmnz.19870630102.

    Article  Google Scholar 

  22. Peacock AD, Sudd JH, Baxter AT: Studies in Pharaoh's ant, Monomorium pharaonis (L.). 11. Colony foundation. Entomol Mon Mag. 1955, 91: 125-129.

    Google Scholar 

  23. Edwards JP: The Biology, Economic Importance, and Control of the Pharaoh's Ant. Economic Impact and Control of Social Insects. Edited by: Bradleigh Vinson S. 1986, New York: Praeger Publishers, 257-271.

    Google Scholar 

  24. Hölldobler B, Wilson EO: The number of queens: an important trait in ant evolution. Naturwissenschaften. 1977, 64: 8-15. 10.1007/BF00439886.

    Article  Google Scholar 

  25. Petersen-Braun MD: Intraspecific aggression in pharaoh ant Petersen-Braun MD: Monomorium pharaonis L (Hymenoptera, Formicidae). Insect Soc. 1982, 29: 25-33. 10.1007/BF02224525.

    Article  Google Scholar 

  26. Taylor B: The Ants of (sub-Saharan) Africa. Ants of Africa, [http://antbase.org/ants/africa/]

  27. Wetterer JK: Worldwide spread of the destroyer ant, Monomorium destructor (Hymenoptera: Formicidae). Myrmecol News. 2009, 12: 97-108.

    Google Scholar 

  28. Petersen-Braun MD: Untersuchungen zur Socialen Organisation der Pharaoameise Monomorium pharaonis (L.) (Hymenoptera, Formicidae). PhD Thesis. 1973, Rheinischen Friedrich-Wilhelms-Universität, Hohen Mathematish-Naturwissenschaftlichen Fakultät

    Google Scholar 

  29. Goudet J: FSTAT, a program to estimate and test gene diversities and fixation indices. 2002, 2.9.3.2

    Google Scholar 

  30. Hedrick PW: A Standardized Genetic Differentiation Measure. Evolution. 2005, 59: 1633-1638.

    Article  CAS  PubMed  Google Scholar 

  31. Jost L: GST and its relatives do not measure differentiation. Mol Ecol. 2008, 17: 4015-4026. 10.1111/j.1365-294X.2008.03887.x.

    Article  PubMed  Google Scholar 

  32. Heller R, Siegismund HR: Relationship between three measures of genetic differentiation GST, DEST and GST: how wrong have we been?. Mol Ecol. 2009, 18: 2080-2083. 10.1111/j.1365-294X.2009.04185.x.

    Article  CAS  PubMed  Google Scholar 

  33. Garza JC, Williamson EG: Detection of reduction in population size using data from microsatellite loci. Mol Ecol. 2001, 10: 305-318. 10.1046/j.1365-294x.2001.01190.x.

    Article  CAS  PubMed  Google Scholar 

  34. Corander J, Marttinen P, Siren J, Tang J: Enhanced Bayesian modelling in BAPS software for learning genetic structures of populations. BMC Bioinformatics. 2008, 9: 539-10.1186/1471-2105-9-539.

    Article  PubMed Central  PubMed  Google Scholar 

  35. Pedersen JS, Krieger MJB, Vogel V, Giraud T, Keller L: Native supercolonies of unrelated individuals in the invasive Argentine ant. Evolution. 2006, 60: 782-791.

    Article  PubMed  Google Scholar 

  36. Vogel V, Pedersen JS, Giraud T, Krieger MJB, Keller L: The worldwide expansion of the Argentine ant. Diversity Distrib. 2010, 16: 170-186. 10.1111/j.1472-4642.2009.00630.x.

    Article  Google Scholar 

  37. Chao A: Nonparametric estimation of the number of classes in a population. Scand J Stat. 1984, 11: 265-270.

    Google Scholar 

  38. Chao A, Shen T-J: Program SPADE (Species Prediction And Diversity Estimation). 2003

    Google Scholar 

  39. Goudet J: PCA-GEN, a program to perform Principal Component Analysis (PCA) on gene frequency data. 2000, 1.3.1

    Google Scholar 

  40. Aitchison J: The statistical analysis of compositional data. 1986, London: Chapman and Hall

    Chapter  Google Scholar 

  41. Ugelvig LV, Drijfhout FP, Kronauer DJC, Boomsma JJ, Pedersen JS, Cremer S: The introduction history of invasive garden ants in Europe: Integrating genetic chemical and behavioural approaches. BMC Biol. 2008, 6: 14-10.1186/1741-7007-6-11.

    Article  Google Scholar 

  42. Errard C, Delabie J, Jourdan H, Hefetz A: Intercontinental chemical variation in the invasive ant Wasmannia auropunctata (Roger) (Hymenoptera Formicidae): a key to the invasive success of a tramp species. Naturwissenschaften. 2005, 92: 319-323. 10.1007/s00114-005-0628-y.

    Article  CAS  PubMed  Google Scholar 

  43. Brandt M, Van Wilgenburg E, Tsutsui ND: Global-scale analyses of chemical ecology and population genetics in the invasive Argentine ant. Mol Ecol. 2009, 18: 997-1005. 10.1111/j.1365-294X.2008.04056.x.

    Article  CAS  PubMed  Google Scholar 

  44. Dani FR, Jones GR, Destri S, Spencer SH, Turillazzi S: Deciphering the recognition signature within the cuticular chemical profile of paper wasps. Anim Behav. 2001, 62: 165-171. 10.1006/anbe.2001.1714.

    Article  Google Scholar 

  45. Akino T, Yamamura K, Wakamura S, Yamaoka R: Direct behavioral evidence for hydrocarbons as nestmate recognition cues in Formica japonica (Hymenoptera: Formicidae). Appl Entomol Zool. 2004, 39: 381-387. 10.1303/aez.2004.381.

    Article  CAS  Google Scholar 

  46. Greene MJ, Gordon DM: Structural complexity of chemical recognition cues affects the perception of group membership in the ants Linephithema humile and Aphaenogaster cockerelli. J Exp Biol. 2007, 210: 897-905. 10.1242/jeb.02706.

    Article  CAS  PubMed  Google Scholar 

  47. Brandt M, van Wilgenburg E, Sulc R, Shea K, Tsutsui N: The scent of supercolonies: the discovery, synthesis and behavioural verification of ant colony recognition cues. BMC Biol. 2009, 7: 71-10.1186/1741-7007-7-71.

    Article  PubMed Central  PubMed  Google Scholar 

  48. Suarez AV, Holway DA, Liang DS, Tsutsui ND, Case TJ: Spatiotemporal patterns of intraspecific aggression in the invasive Argentine ant. Anim Behav. 2002, 64: 697-708. 10.1006/anbe.2002.4011.

    Article  Google Scholar 

  49. Liang D, Blomquist GJ, Silverman J: Hydrocarbon-released nestmate aggression in the Argentine ant, Linepithema humile, following encounters with insect prey. Comp Biochem Physiol B Biochem Mol Biol. 2001, 129: 871-882. 10.1016/S1096-4959(01)00404-3.

    Article  CAS  PubMed  Google Scholar 

  50. Carlin NF, Holldobler B: The kin recognition system of carpenter ants (Camponotus Spp) .1. Hierarchical cues in small colonies. Behav Ecol Sociobiol. 1986, 19: 123-134. 10.1007/BF00299947.

    Article  Google Scholar 

  51. Caldera EJ, Holway DA: Evidence that queens do not influence nestmate recognition in Argentine ants. Insect Soc. 2004, 51: 109-112. 10.1007/s00040-003-0716-y.

    Article  Google Scholar 

  52. Vander Meer RK, Alonso LE: Queen primer pheromone affects conspecific fire ant (Solenopsis invicta) aggression. Behav Ecol Sociobiol. 2002, 51: 122-130. 10.1007/s002650100417.

    Article  Google Scholar 

  53. Steiner FM, Schlick-Steiner BC, Moder K, Stauffer C, Arthofer W, Buschinger A, Espadaler X, Christian E, Einfinger K, Lorbeer E, et al: Abandoning aggression but maintaining self-nonself discrimination as a first stage in ant supercolony formation. Curr Biol. 2007, 17: 1903-1907. 10.1016/j.cub.2007.09.061.

    Article  CAS  PubMed  Google Scholar 

  54. Nunney L: Lineage selection: natural selection for long-term benefit. Levels of Selection in Evolution. Edited by: Keller L. 1999, New Jersey: Princeton University Press, 238-252.

    Google Scholar 

  55. Crozier RH: Genetic Clonal Recognition Abilities in Marine Invertebrates Must be Maintained by Selection for Something Else. Evolution. 1986, 40: 1100-1101. 10.2307/2408769.

    Article  Google Scholar 

  56. Ratnieks FLW: The Evolution of Genetic Odor-Cue Diversity in Social Hymenoptera. Am Nat. 1991, 137: 202-226. 10.1086/285154.

    Article  Google Scholar 

  57. King JR, Tschinkel WR: Experimental evidence that human impacts drive fire ant invasions and ecological change. Proc Natl Acad Sci USA. 2008, 105: 20339-20343. 10.1073/pnas.0809423105.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  58. King JR, Tschinkel WR, Ross KG: A case study of human exacerbation of the invasive species problem: transport and establishment of polygyne fire ants in Tallahassee, Florida, USA. Biol Invasions. 2009, 11: 373-377. 10.1007/s10530-008-9254-x.

    Article  Google Scholar 

  59. Nakamaru M, Beppu Y, Tsuji K: Does disturbance favor dispersal? An analysis of ant migration using the colony-based lattice model. J Theor Biol. 2007, 248: 288-300. 10.1016/j.jtbi.2007.05.012.

    Article  PubMed  Google Scholar 

  60. Tsuji K: What brings peace to the world of ants (Hymenoptera: Formicidae)?. Myrmecol News. 2010, 13: 130-132.

    Google Scholar 

  61. Reeve HK: The Evolution of Conspecific Acceptance Thresholds. Am Nat. 1989, 133: 407-435. 10.1086/284926.

    Article  Google Scholar 

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Acknowledgements

We thank Cedric Collingwood for help in species identification, David Nash and Luke Holman for assistance in analysing cuticular hydrocarbon data, Pia Gertsch, Daniel Kronauer, and Sylvia Mathiasen for help with developing primers for the microsatellite loci, Andreas Kelager for help with the BAPS analyses, and Jérôme Goudet, Pekka Pamilo, and Hans Siegismund for discussions on the genetic data. Diane Allard, Ehab Abouheif, Boris Baer, Howard Bell, Lisbeth Børgesen, the Cobblah-group, Tina Frisch, Aleksandra Gliniewicz, Chow-Yang Lee, Say-Piau Lim, Yeo Kolo, Alexander Mikheyev, David Oi, Fabien Ravery, Tsong Hong Su, Brian Taylor and Kazuki Tsuji kindly provided ant samples. Luke Holman, Tim Linksvayer, Nathan Sanders, and two anonymous referees gave valuable comments on previous versions of this manuscript. This study was supported by the Clément Foundation (AMS), CODICES Marie Curie Excellence Grant, contract number MEXT-CT-2004-014202 (PdE), the Danish Natural Science Research Council (JSP) and the Danish National Research Foundation (PdE, JSP). The experiments performed comply with Danish law.

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Correspondence to Anna M Schmidt.

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Authors' contributions

AMS collected the ants and conducted the field work, carried out the behavioural assays and genetic lab work and analyses, participated in the chemical analyses and the design of the study and drafted the manuscript. PdE participated in the chemical analyses. JSP participated in the design of the study. All authors revised the draft manuscript and approved the final manuscript.

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12983_2010_140_MOESM1_ESM.DOC

Additional file 1: Ward trees based on hierarchical cluster analyses of CHC data (a) Monomorium pharaonis and (b) M. pharaonis and M. destructor. ID codes for M. pharaonis are like those given in Table 1, and the M. destructor samples have been added as Md1, Md2, and Md3. (a) was constructed in JMP.IN 5.1 based on Euclidian distance measures obtained from 18 identified cuticular hydrocarbons in M. pharaonis (see Methods for further details). The colours have been randomly assigned to the different branches. (b) was constructed in JMP 8.0 based on simple presence-absence data of the 26 cuticular hydrocarbons found in M. pharaonis and M. destructor combined; the M. destructor branches have been marked purple. (DOC 32 KB)

12983_2010_140_MOESM2_ESM.XLS

Additional file 2: Estimates of pairwise genetic distances ( F ST ) between sampled colonies of Monomorium pharaonis based on four microsatellite loci analysed and calculated in FSTAT (Table 2). (XLS 38 KB)

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Schmidt, A.M., d'Ettorre, P. & Pedersen, J.S. Low levels of nestmate discrimination despite high genetic differentiation in the invasive pharaoh ant. Front Zool 7, 20 (2010). https://doi.org/10.1186/1742-9994-7-20

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