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Table 2 Microsatellite loci developed and analysed in this study

From: Low levels of nestmate discrimination despite high genetic differentiation in the invasive pharaoh ant

Locus

Primer sequences (5'-3')

Repeat motif

GenBank accession no.

No. of alleles

Size range (bp)

Mp1

f: GCCAATGGTTTAATCCCTCA

(AG)3AA(AG)23

HM587312

15

192-279

 

r: TCATACTGCGTGTGCCTTTC

    

Mp3

f: ACAAGGTAAGTCGCCACCAT

(GT)20

HM587313

9

127-147

 

r: TCGTGATAATTCGCGATGAA

    

Mp12

f: TGGCCAAAAGTATCCAGGAG

(AC)8

HM587314

2

130-132

 

r: TCGTCGAAAGTATCGAAGTAAAC

    

Mp13

f: CCCATTGAGATTGCGGCAT

(AC)10(GC)8ACA(AC)10

HM587315

9

281-298

 

r: GCACAGGCACGTAACGATT

    
  1. Data on allele number and size range are for the total sample of 35 Monomorium pharaonis colonies analysed (n = 1026 individuals).
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Frontiers in Zoology

ISSN: 1742-9994