- Open Access
Landscape homogenization due to agricultural intensification disrupts the relationship between reproductive success and main prey abundance in an avian predator
Frontiers in Zoology volume 16, Article number: 31 (2019)
Selecting high-quality habitat and the optimal time to reproduce can increase individual fitness and is a strong evolutionary factor shaping animal populations. However, few studies have investigated the interplay between land cover heterogeneity, limitation in food resources, individual quality and spatial variation in fitness parameters. Here, we explore how individuals of different quality respond to possible mismatches between a cue for prey availability (land cover heterogeneity) and the actual fluctuating prey abundance.
We analyse timing of breeding and reproductive success in a migratory population of Eurasian kestrels (Falco tinnunculus) breeding in nest-boxes, over a full three-year abundance cycle of main prey (voles), and consider several components of individual quality, including body condition, blood parasite infection, and genetic diversity (n = 448 adults) that act on different time scales. Older individuals, and kestrel parents in higher body condition started egg-laying earlier than younger birds and those in lower body condition. Additionally, egg-laying was initiated earlier during the increase and decrease phases (2011 and 2012) than during the low phase of the vole cycle (2013). Nestling survival (ratio of eggs that fledged successfully) was higher in early nests and in heterogeneous landscapes (i.e., mosaic of different habitat types), which was evident during the increase and decrease phases of the vole cycle, but not during the low vole year.
We found a strong positive effect of landscape heterogeneity on nestling survival, but only when voles were relatively abundant, whereas a difference in the timing of breeding related to territory landscape heterogeneity was not evident. Therefore, landscape heterogeneity appeared as the main driver of high reproductive performance under favourable food conditions. Our results show that landscape homogenization linked to agricultural intensification disrupts the expected positive effect of vole abundance on reproductive success of kestrels.
Selecting the right time and place to reproduce is an essential decision for animals. It will affect their reproductive output and long-term survival, and as a result what we refer to as individual fitness [1, 2]. For territorial birds, the fittest individuals are expected to preferentially occupy the higher-quality sites, while less competitive individuals occupy poorer sites (‘Ideal Dominance Distribution model’, [3,4,5]). In migratory species, earlier-arriving individuals are usually of higher individual quality (for instance older and/or in better body condition) than later-arriving ones and settle on progressively lower-quality territories (‘sequential settlement’, see ). Early arrival may therefore be advantageous in terms of reproductive performance (reviewed in ).
Under habitual environmental conditions, one would expect territories occupied first to show highest breeding success as a combination of higher quality individuals occupying higher quality territories. But rapid global changes, such as climate change and land-use changes, have severely altered the relationship between species and their environment [7, 8]. Large-scale changes in land-cover can have detrimental effects on species either through habitat loss and fragmentation [9, 10], or in a more devious manner, by uncoupling cues used to select a suitable habitat, and the true value of this habitat . This type of uncoupling has been shown in many ground-nesting birds that persist to breed in intensive farmlands because they are lured by cues that used to be appropriate in pristine open habitats . For example, Northern Wheatears (Oenanthe oenanthe) use field layer height as a cue for quality, but height also correlates with the temporal proximity to harvesting. Thus, intensive farmlands are associated with poor reproductive success due to agricultural activities destroying clutches or broods, or reducing adult survival [12, 13]. Identifying the existence of cue mismatches and unravelling the mechanisms behind them is necessary to fully understand and predict the impacts of land-use changes on population dynamics.
One main challenge in unravelling mechanisms behind potential mismatches between perceived quality and realised fitness, is the biologically valid definition and quantification of habitat quality and individual quality. Studies that combine both measures are still rare (but see for example [5, 14, 15]). Additionally, such individual quality measurements might operate on varying time scales. For example, body condition may vary in time and as a consequence captures only a relatively short time period. Blood parasite infection would act on a medium time frame which incorporates the exposure and susceptibility to haemosporidian parasites. Gene diversity expressed by individual genetic heterozygosity would be constant over time. In more detail, body condition is a measure of fat content or nutrient reserves in relation to body size. It can indicate periods of nutritional stress [16, 17] and might vary between and within years (i.e., over the course of a breeding season; ). Habitat composition might also determine the exposure to vectors of blood parasites [19, 20]. At the same time, if a bird’s immune system is compromised due to nutritional stress  or breeding effort , the infection risk (probability of infection, estimated from the presence or absence of haemoparasites at the individual level) might increase. Both, exposure and susceptibility to vector-borne parasites will thus reflect the quality of a birds’ settlement decision but also the individual quality of the breeding adult that might again vary over time. Infection status might further shape reproductive investment strategies and decrease reproductive success, since individual’s defence against parasites is costly . Lastly, individual genetic heterozygosity (i.e., proportion of genetic loci bearing two different alleles, reflecting the genetic diversity of an individual) is known to affect fitness-related traits (see heterozygosity–fitness correlations; e.g. [24,25,26]). For example, heterozygosity (estimated by microsatellites) in females can be positively associated with clutch and egg sizes . Heterozygous mates often provide larger food items  in higher feeding rates . Another commonly used individual quality measurement is the age of the breeding adults, that relates to breeding experience, and is well known to influence both the timing of breeding and breeding success (as for example documented in our study system, e.g.: [30,31,32]).
European farming policies have led to a collapse in farmland bird populations . The Eurasian kestrel (Falco tinnunculus, hereafter kestrel) is no exception to this general negative trend [34, 35]. In Finland, kestrel populations collapsed because of pesticides in late 1950s and 1960s, and have then been increasing during 1980s to 2000s thanks to the provisioning of suitable nest-boxes in certain areas . In Northern Europe, kestrels are long-distance migrants that over-winter in Southern Europe, North Africa and the Sahel region [37, 38]. They are capable of breeding in various habitats as long as open landscape for hunting is available. We hypothesize that early migrants prefer to settle in open habitats, generated by intensive agricultural practices, because in boreal areas, heterogeneous habitats with forested patches are expected to retain snow for a longer period than open farmlands. Indeed many studies have investigated the effects of vegetation types on snow accumulation and melting processes and have shown that snow melt rates are slower in forested landscapes ([39,40,41]; and see  for a thorough review of the empirical evidence in the literature). The early disappearance of snow in homogeneous agricultural fields could hence be an anthropogenically induced cue for habitat quality because voles, kestrels’ main prey [38, 43], are more easily detectable in snow-free patches during the settlement phase . However, this cue can be biased on some years, depending on the phase of the vole cycle which follows a high-amplitude 3-year population cycle in western Finland . In low vole abundance years, it is likely that homogeneous agricultural habitats are of lower quality for kestrels than more heterogeneous habitats, where more alternative prey species (birds, shrews, lizards and insects) can be found [46, 47]. Additionally, organic farming (following the agri-environment-climate schemes of the European Union, including reducing land-use intensity and maintaining or introducing biodiversity-rich habitats) and the amount of non-field grassland are positively correlated to total bird abundance across Finland . Kestrels are indeed known to hunt in forests and clear-cut areas during poor vole years . Therefore, this species offers a unique opportunity to disentangle the underlying mechanisms of how variations in landscape heterogeneity and main prey abundance may affect the reproductive success of an avian predator depending on individual quality.
Here, we explore how individuals of different quality respond to possible mismatches between a cue for prey availability (land cover heterogeneity) and the actual fluctuating prey abundance. We used laying date as a proxy of time of settlement, and nestling survival (the ratio of laid eggs that successfully fledged) as a proxy for breeding investment versus breeding performance. We expected high-quality individuals to arrive earlier on breeding grounds and to settle in more homogeneous agricultural landscapes where snow melts earlier and where prey might be more accessible. We predicted that (i) egg-laying initiates earlier in homogeneous agricultural fields; and, (ii) high-quality individuals start egg-laying earlier in more homogeneous landscapes, and low-quality individuals start egg-laying later in more heterogeneous landscapes. We further predicted these patterns to be independent of the vole cycle: (iii) high-quality individuals manage to have high nestling survival in homogeneous landscapes when voles are abundant, but they are ‘trapped’ in these territories with few alternative prey during low vole abundance years, which will be seen in low nestling survival. In turn (iv) low-quality individuals have higher nestling survival than high-quality individuals in heterogeneous landscapes during low vole abundance years only. Finally, we predicted (v) that short-term individual quality measures and breeding experience (parental age) have a stronger influence under such fluctuating food conditions than long-term individual quality measurements.
The study area is situated in the Kauhava and Lapua region, western Finland (62°59′-63°10′N, 22°50′- 23°20′E, see ). It consists of a mix of two contrasted habitats: the first is a homogeneous open habitat (mostly in the West, > 70% agricultural fields) and the second is a heterogeneous habitat (mostly in the East, 25–30% agricultural fields interspersed with exploited coniferous forests and clear-cuts; Fig. 1a). A majority (60–70%) of agricultural fields of the study area are sown every spring with mostly oats and barley, and correspondingly a minority (30–40%) of agricultural fields are permanently producing grass for silage and hay. Cereal fields are usually ploughed already in autumn. Over the course of the 3-years study period, we did not see any changes in temperature, precipitation or snow cover. For an overview of long-term weather data, see Fig. 3.5. in .
Yearly land cover maps were produced by using Landsat images in a resolution of 30x30m (see  for details on how the maps were generated). Landscape heterogeneity was defined as the diversity of land covers in a kestrel territory and was calculated with Simpson’s Index (see ) in the ‘vegan’ package , by including the number of land cover categories present and the percentage area of the following types: built-ups and roads, peatland bogs, agricultural fields, water, clear-cuts, young forest, mature and old-growth forest (more details can be found in Additional file 1: S1 and in Appendix S2 in ). Forests were classified according to their wood volume, following the Finnish Forest Resources Institute classification: clear cuts: < 52 m3/ha, young forests 52–101 m3/ha, mature forests 102–151 m3/ha and old forests > 152 m3/ha. We used the average inter-nest distance between occupied boxes as a proxy for the diameter of kestrel territories (i.e., an average of all nest distances to their closest neighbour) and hence as buffer radius to characterise landscape heterogeneity in each territory. The vole cycle highly influences the breeding density of kestrels , to account for this varying density, we varied the buffer scale accordingly between years: r(2011) = 650 m (132.7 ha); r(2012) = 694 m (151.3 ha), r(2013) = 843 m (223.3 ha). We used the Simpson’s Index to quantify Territory Land Cover Heterogeneity (see  for a similar method of characterising raptor territory composition), hereafter called ‘TLCH’, whereby lower values represent more homogeneous landscapes, and higher values more heterogeneous landscapes (Fig. 1b). Homogeneous landscapes mainly consist of large agricultural fields (correlation between TLCH and farmland areas plotted in Additional file 1: S2). Additionally, we measured the distance between the nest box and the closest forest edge to account for potential higher predation risk imposed by larger forest predators such as Northern goshawks (Accipiter gentilis) and Pine martens (Martes martes). These two species often depredate kestrel nests and adults . Real and perceived predation risk can affect breeding habitat selection, onset of breeding, and clutch size in a range of species (e.g. [57,58,59,60]). In addition, it has previously been shown that kestrel nest boxes located close to forest edges have a lower occupancy rate than nest boxes located away from forest edges , perhaps as a response to the perceived risk of predation near forests. Finally, we controlled for potential density dependent effects on laying date and breeding success by including the nearest neighbour distances (NND, in meters, log transformed) between occupied nest boxes in the analyses. We chose NND because studies exploring different measurements found the distance to nearest neighbours to be the best predictor for density dependence (e.g., ).
Vole density index
The main prey items of kestrels in our study site are voles of the genera Microtus (the field vole Microtus agrestis and the sibling vole, also called southern vole, Microtus rossiaemeridionalis) and Myodes (the bank vole Myodes glareolus, ). Their abundance is highly determined by the phase of the high-amplitude 3-year vole cycle [45, 63]. No long-term temporal trend in abundance of Microtus and bank voles could be detected in our study area and regular three-year cyclic fluctuations of these voles have been evident from 1980s onwards up to 2010s (Additional file 1: Figure S5; ). The phase of the vole cycle largely determines breeding density and performance of kestrels: breeding density is higher, egg-laying starts earlier, clutch sizes are larger, and breeding success is higher in the increase and decrease phases than in the low phase of the 3-year vole cycle [50, 65]. We covered a complete cycle between 2011 and 2013 and estimated vole abundance indices by bi-annual trapping (Fig. 1c). Rodent abundance in the study area is spatially synchronous (over 70–500 km, see [66,67,68,69]) and does not show differences between the two types of habitats, which is also known from other boreal study systems that undergo strong population cycles in vole abundance . Snap traps were set up in mid-September (autumn) and early May (spring) in two sites 14 km apart in the study area. In each of these two sites, four plots were sampled in the main habitat types including cultivated fields, abandoned fields, spruce forests and pine forests (see  for details). Between 50 and 60 baited Finnish metal mouse snap traps were set at 10-m intervals in vole runways on each plot and were checked daily for 3 consecutive days. Thus, the area of a sample plot ranged from 0.5 to 0.6 ha. We pooled the trapping results for Microtus voles from four-night trapping periods and standardised them to the number of animals caught per 100 trap nights in each habitat type. These data are thereafter referred to as ‘vole index’ in ‘spring’ and ‘autumn’. The vole cycle in the study area has been documented with this method since 1973 (see long-term Microtus vole index for the study area in Additional file 1: S3).
In the study area, kestrels have been breeding in nest-boxes fastened on the gables of barns since the early 1980’s. The proportion of tree breeders is below 10% (EK, pers. obs.). Between 2011 and 2013, a total number of 358 (2013), 363 (2012) and 374 (2011) kestrel nest-boxes were monitored in the study area. The study population is known for its high annual turn-over rate of 82%, together with a high divorce rate (on average 90% females and 68% males are new in our study area, pooled data from 1985 to 2010; see ). From the beginning of the breeding season (late April to early May), nest-boxes were inspected 3 to 4 times per breeding season to record the occupancy, egg-laying date, clutch size and number of fledged young of all active nests while minimizing nest disturbance. Kestrels start incubation after the second egg is laid, therefore the lay date can be estimated by subtracting 30 days from the estimated date of hatching , which can be determined on the basis of wing lengths of nestlings for successful nests . For unsuccessful nests, we used egg-floating, a method well-established in waders [73,74,75] that was modified for kestrels to estimate approximate day of hatching. Parents were trapped at the nests by using a swing-door trap attached on the entrance hole of the nest box when chicks were 2–3 weeks old (see  for details on morphometric measurements and blood sampling). They were ringed and aged according to [76, 77]. We obtained morphometric measurements for the body condition index and blood samples for Haemoproteus sp. infection and genotyping of 448 individuals (n(2011) = 139, n(2012) = 163 and n(2013) = 151 adults). In our 3-year data set, we did not have any repeated measurements of breeding adults, which is due to long breeding dispersal distances and the high turnover rate of breeding individuals in the study population .
We quantified indices for individual quality that act on different time scales: i) body condition; ii) blood parasite infection, iii) genetic diversity; and iv) age as a proxy for breeding experience.
I) The residuals of the regression of body mass on wing length (both log transformed), sex and the time (in days) between the capture date and laying date were used as an index of body condition (abbrev. ‘bc’; ), in order to account for the size dimorphism of the species (females are larger than males) and the decrease in body mass of adults throughout the breeding season [18, 79]. Lower values reflect individuals in lower body condition and vice versa; see  for a similar approach.
II) DNA extractions of collected blood samples were done in the Center of Evolutionary Applications, University of Turku, and were subsequently genetically screened for blood parasites of the genera Haemoproteus, Plasmodium and Leucocytozoon at the Molecular Ecology and Evolution Lab, Lund University, Sweden. The exact protocol based on a nested PCR can be found in Additional file 1: S4. Only Haemoproteus nisi was detected with a sufficient prevalence and was included as the blood parasite infection risk variable (presence/absence in an individual adult). Blood parasite prevalence does not change throughout an individuals’ life once an infection occurred, as opposed to infection intensity, that varies over time .
III) The laboratory work for individual genetic heterozygosity was done in the Central Laboratories of the Natural History Museum Vienna, Austria and was based on 22 different microsatellites established for Falco peregrinus  and F. naumanni [83, 84]. Details on the primers used and the multiplex PCR protocol can be found in Additional file 1: S5, the exact procedure to i) determine final allele sizes; ii) identify outliers; iii) test for potential scoring errors, allelic dropout and null alleles; iv) departures from Hardy-Weinberg equilibrium; and, v) genotypic linkage disequilibrium can be found in Additional file 1: S6. After this quality assessment, the remaining 17 microsatellites were used to estimate five commonly used measures of individual multilocus heterozygosity using the GENHET R function . However, because of the high inter-correlation among these measures (Spearman correlations: |r| > 0.95, P < 0.001), we chose only one, the standardized heterozygosity . This is a widely used and effective measure of genetic diversity, computed as the proportion of heterozygous loci for a given individual divided by the average of the population-level mean heterozygosity for those same loci (; see [86, 87] for a similar approach), and is used hereafter as ‘Hs_exp’, whereby lower values reflect lower genetic heterozygosity.
IV) The age estimation in kestrels is only possible between first year breeders and older individuals (see [76, 77]). Age was hence included as a two-level factor variable in our analyses. In raptors, young breeders usually start egg-laying later (e.g., [30, 50]) and have lower breeding success (e.g., [31, 32]). As mentioned before, the high turn-over and divorce rate in our study population does not leave us with many ringed adults that would allow a more exact age estimation.
To analyse how individual quality, territory land cover heterogeneity and vole abundance influence the timing of breeding (estimated as lay date; to test predictions i and ii) and breeding performance (estimated as nestling survival, to test predictions iii and iv) we used an information theoretic approach and model averaging with the R packages ‘lme4’ , “MASS” and ‘MuMIn’ ; see [90, 91] for details on multi-model inference. Model averaging was the appropriate approach, as no single model was strongly supported for either response variable . The vole indices in spring and autumn were correlated (r = 0.50), thus we initially fitted competing models and decided to use the vole abundance in spring as the predictor variable throughout because of higher explanatory capacity and because the index in spring, when kestrels arrive on territory, was more biologically meaningful from the point of view of kestrels making settlement decisions. The vole index was fitted as an ordered factor in the statistical analyses. Results in the model output are thus displayed for the linear or quadratic relationship between the 3 study years/vole abundances.
Lay date (Julian day of clutch initiation, following a normal distribution) was analysed using LMMs with a Gaussian error distribution and identity link function and the following fixed-effects: individual quality (either body condition index, Haemoproteus sp. infection risk or individual genetic heterozygosity [Hs_exp] as models tend to fail to converge if all three uncorrelated variables were included at once), age (factor with two levels: first year breeder or older), TLCH and the vole index in spring, together with the interaction terms between individual quality*TLCH, individual quality*vole index, and vole index*TLCH. Finally, the models included the distance to the closest forest edge (dist) and the nearest neighbour distances (NND) as additional fixed effects. ‘Nest box ID’ was included as random factor. In the lay date analyses all breeding records (successful and failed nests) were included.
Nestling survival was estimated as the ratio of laid eggs that successfully fledged. Some eggs that did not result in fledglings may have been infertile, which may vary according to habitat composition (e.g., ), thus our variable was a combination of egg-hatch ratio and hatch-fledged ratio, but hatching numbers were not known for all broods, explaining why we could not separate between the two contributing factors. It was analysed using GLMMs with a binomial denominator. We used the same fixed-effects as for the timing of breeding [individual quality (either body condition index, Haemoproteus sp. infection risk or Hs_exp), age, TLCH, vole index, individual quality*TLCH, individual quality*vole index, vole index*TLCH, dist and NND], with the relative lay date (centred to the mean of the study year) as an additional predictor variable because of the known decline in clutch size with later laying date in kestrels . ‘Nest box ID’ was again included as a random factor. In the nestling survival analyses, only successful nests that fledged at least one young were included. We found 8 complete nest failures during the study period, that happened after adult trapping (when nestlings are 2–3 weeks old) and might have been due to predation.
All fixed effect covariates were tested beforehand for correlations; and with the exception of the two vole indices, no strong correlations were found (and all predictors with rho< 0.4 were maintained as covariates). Predictor variables contained no missing values, ensuring accurate model comparisons throughout the selection and averaging process . A global model was fitted with any strongly correlated explanatory variables; all quantitative variables were scaled and centered, ensuring that effect sizes were on a comparable scale . We generated a candidate list using all possible combinations of the predictors outlined above. Additionally, the appropriate null models (i.e., random factors ‘nest box ID’ only, and yearly variation ‘vole index’ only), were considered in the candidate list (but never featured into any of the top models). See Additional file 1: S7 for the complete candidate lists. (Note that the vole index and ‘year’ were fully confounded (r = 1.0), which is why study year was not further considered in the candidate models, and could also not be considered as a random term because 3 levels are not sufficient . But because of the full correlation between ‘year’ and vole abundance, the yearly variation is fully accounted for throughout. Each candidate model was compared to one another using Akaike Information Criterion values, corrected for small sample size (AICc) in the package ‘AICcmodavg’ . Akaike weights (ωi) were calculated to assess the relative likelihood for each model considered ; thus, ωi reflect the model probability given the full model list rather than only those below a given threshold of ΔAICc. All models with ΔAICc < 4.0 were extracted and consequently used for model averaging . We report the direction of parameter estimates and their magnitudes (effect sizes), unconditional SEs and CIs (95% confidence intervals) from model averaged coefficients, and the variable’s relative importance (RVI; i.e., model probability for each explanatory variable tested; [98, 100]. Unconditional SEs incorporate model selection uncertainty, as opposed to standard SEs which only consider sampling variance [90, 91]. We used CIs to assess the magnitude of the effect and conclude that the estimate is different from zero (i.e., there is a significant effect) when the CIs exclude zero. Posthoc comparisons between factor variables in interaction terms were performed using the package ‘emmeans’ . All statistical analyses were performed with the software R version 3.4.4  unless stated otherwise. The confidence intervals were set at 95% (corresponding to a significance level of P = 0.05) for all tests conducted.
For both model selection processes, we visually inspected residual distribution to assess model fit and tested for potential spatial autocorrelation in all response variables by using Moran’s I (“ape” package, ) and visual inspection of spatial plots and variograms of residuals (“gstat” package, ). We found no indication for spatial autocorrelation throughout (P ∈ [0.36; 0.82]). We present spatial plots for the individual quality indices i) body condition (Gaussian distribution); (ii) Haemoproteus infection risk (binomial distribution); and, (iii) Hs_exp (Gaussian distribution) for both sexes in Additional file 1: S8.
The year 2011 was an increase phase of the vole cycle (Microtus vole index autumn 2010 = 1.29, spring 2011 = 9.58, autumn 2011 = 16.2) with a following decrease phase (spring 2012 = 3.36, autumn 2012 = 0.28). The next year (2013) was a low vole year (spring 2013 = 0 and autumn 2013 = 0.17 Microtus voles trapped per 100 trap nights). Nest box occupancy heavily depended on the phase of the vole cycle. In 2011, the increase phase of the vole cycle, 222 nest boxes were occupied (59%), 219 of which successfully fledged young (98%). In 2012, the decrease phase of the vole cycle, 199 nest boxes were occupied (55%), all of which were successful (100%); and in 2013, the low vole year, 121 nest boxes were occupied (34%), 98 of which successfully fledged young (81% of occupied boxes). Note that we could not successfully trap adults in all these nest boxes, which is why sample sizes differ from the data set used in this study.
We had a complete dataset on body condition, blood parasite infection and individual genetic heterozygosity from 448 adults (n(2011) = 139, n(2012) = 163 and n(2013) = 151) with no repeated measurements (i.e., different breeding adults sampled throughout the study period), obtained from 190 different nest-boxes over 3 years. The number of first year breeders was low, especially during the low vole year (n(2011) = 34, n(2012) = 31 and n(2013) = 6).
The initiation of egg-laying (lay date) was determined by the age of the breeding adult, individual body condition and the phase of the vole cycle (Table 1). Egg-laying was on average 3.29 ± 0.78 SE days earlier in older parents compared to first-year breeders (Fig. 2a; least-square means post-hoc contrast: t-ratio = 4.22, P < 0.001). Individuals in higher body condition started egg-laying earlier than individuals in lower body condition (Fig. 2b). Egg-laying was initiated on average 5.76 ± 0.72 SE and 4.76 ± 0.78 SE days earlier during the increase and decrease phases (2011 and 2012), respectively, compared to the low phase (2013) of the vole cycle (Fig. 2c) (vole index = 0 and 3.36 post-hoc contrast: t-ratio = 8.05, P < 0.001; vole index = 0 and 9.58 post-hoc contrast: t-ratio = 6.11, P < 0.001; vole index = 3.36 and 9.58 post-hoc contrast: t-ratio − 1.41, P = 0.340).
We found higher nestling survival (Table 2) in earlier nests (Fig. 3) and during the decrease (2012) phase compared to the increase (2011) and low (2013) phases of the vole cycle, but this relationship was only evident in older breeders (vole index = 3.36, first year and older post-hoc contrast: odd ratio 0.396 ± 0.11 SE, z-ratio = − 3.21, P = 0.001; Fig. 4a, all least square mean post-hoc contrasts in Additional file 1: S9). The slope of the relationship between nestling survival and the vole cycle was further dependent on territory land cover heterogeneity, in a way that nestling survival was higher in more heterogeneous landscapes (small fields in the East) than in the more homogeneous landscapes (large fields in the West) during the increase (2011) and decrease (2012) phases of the vole cycle. However, this difference was not evident during the low vole year (2013, Fig. 4b).
We addressed variation in individual parental quality, vole abundance and land cover heterogeneity that induced differential onset of breeding (reflecting settlement decisions) and nestling survival (reflecting breeding investment versus breeding output; i.e., fitness consequences). The most interesting finding of our study was a strong positive effect of landscape heterogeneity on nestling survival of kestrels, but only when voles were relatively abundant, whereas a difference in the timing of breeding related to territory landscape heterogeneity was not evident.
As expected, laying dates in our study population were positively correlated to vole abundance as egg-laying initiated earlier during the increase and decrease years of the vole cycle, as shown in previous studies [30, 50, 65]. Egg laying was also earlier in older breeders and those with higher body condition, following the sequential settlement paradigm . Earlier egg-laying in older individuals (reflecting higher individual quality; see for instance ) have already been found in long-term data sets in our study population  and elsewhere [38, 106]. However, contrary to predictions i) and ii), landscape heterogeneity did not influence laying dates at the territory scale. Therefore, early individuals do not seem to settle preferentially in homogeneous landscapes where snow melts earlier. Nevertheless, landscape heterogeneity influenced nestling survival depending on local vole abundance, i.e. nestling survival was higher in more heterogeneous landscapes, but only during the increase and decrease phases of the vole cycle. These results are contradictory to the predictions iii) and iv), suggesting that heterogeneous habitats did not buffer against low abundance of the main prey.
We offer two non-mutually exclusive explanations for this pattern. It may be that i) higher alternative prey abundance boosts nestling survival in these heterogeneous landscapes when voles are abundant; or ii) that there are unexpected fine-scale spatial differences in vole abundance between homogenous and heterogeneous landscapes. Thus, when voles are abundant throughout the study area as shown in our long-term vole cycle data, they might reach even higher densities in heterogeneous territories, a phenomenon that has thus far been shown in western Europe .
More diverse prey communities in heterogeneous landscapes might enhance the reproductive performance of kestrels breeding in these territories when voles are abundant. Kestrels in our study area feed mainly on voles (46% of prey number between 1972 and 1983), followed by insects, shrews, birds, mice, lizards and frogs . This author showed that diet composition depends on the vole cycle, but also on landscape composition, as more shrews and birds were caught in heterogeneous landscapes than in homogenous landscapes. For example, fledglings of forest passerines (mainly Turdus spp.) are an important alternative prey species particularly during low vole abundance years [43, 108].
Maladaptive habitat decision-making
Our results highlighted that kestrels do not choose their territory according to its true value at the time of settlement. For example, egg-laying should be earlier in more heterogeneous landscapes, where nestling survival is highest during high vole abundance years, which was not the case. Although high-quality individuals settle earlier, they seem to settle randomly throughout the study area, at least over the 3-year study period. This suggests that large-scale spatial synchrony in vole fluctuations  is the main factor constraining settlement decisions in nomadic avian predators (see refs on dispersal of nomadic raptors, [71, 80, 109]). However, there might be, as stated above, fine-scale differences in vole abundance that could drive the higher nestling survival in heterogeneous landscapes when vole abundance is generally high to medium (2011 and 2012). The fact that there was no difference in nestling survival between heterogeneous landscapes and homogenous landscapes when vole abundance was generally low (2013), shows that these habitats do not sufficiently buffer against low main prey densities.
Maladaptive habitat decision-making implies a preference for low-quality habitats over high-quality options causing the species to fall into what is known as an ecological trap (reviewed in [11, 110,111,112,113]). Our results appeared not to support the ecological trap hypothesis as no spatial variations in laying dates (as a proxy of settlement decision) depending on landscape heterogeneity could be detected. However, our results strikingly indicate that landscape homogenization linked to agricultural intensification disrupts the expected positive effect of vole abundance on reproductive success of kestrels (see Fig. 4b; nestling survival had similar low predicted values in very homogeneous landscapes in the three different years of the vole cycle).
Effects of different individual quality indices on reproductive performance
The measurement we were specifically interested in was nestling survival, i.e. the clutch size per fledgling ratio, because this variable reflects individual variations in reproductive performance linked to parental quality. We found clear evidence that older breeders performed better in raising their offspring than first year inexperienced breeders, but this was only true during the increase phase of the vole cycle. This might indicate that learning about fluctuating food conditions, and taking advantage of the periodically high vole abundance, is part of the breeding experience that older individuals already gained, similarly to the development of migratory behaviour in the Black Kite Milvus migrans . Additionally, the number of first year breeders in kestrels is especially low during the low vole year , which also underlines the importance of breeding experience to persist under such fluctuating food conditions.
Finally, our fifth prediction, i.e. that short-term individual quality measurements and breeding experience (parental age) have a stronger influence under such fluctuating food conditions than long-term individual quality measurements, was fully met. We indeed found body condition, a highly seasonal estimate, to modify the timing of breeding, but blood parasite infection or individual genetic heterozygosity did not feature into any of the top models. This is also partly in line with our prediction (iv), that those individuals that are in good body condition (high body mass relative to body size) after migration will be able to secure a territory and successfully raise fledglings, but this was not linked to landscape habitat heterogeneity as we expected.
Early arriving kestrels that are older and/or in higher body condition started egg-laying earlier, but did not show a clear habitat preference for either homogeneous agricultural landscapes or more heterogeneous landscapes. However, kestrels breeding in the latter had in fact higher nestling survival than their con-specifics, an effect that was visible only during the increase and decrease phases of the vole cycle. Therefore, the benefit of breeding in heterogeneous habitats that offer alternative prey is not enough to compensate for low vole abundances, as there was no difference in nestling survival between sites in the year of vole scarcity (2013). Unexpectedly, differences in nestling survival between habitats and depending on vole abundance were not mirrored by spatial variations in egg-laying dates. This suggests that other factors are of essence in this kestrel population. For example, large-scale breeding dispersal aiming to track cyclic fluctuations in vole abundance might be more important in determining settlement timing and decisions than landscape heterogeneity. However, landscape heterogeneity appears as a main driver of high reproductive performance under favourable food conditions.
Overall, for rodent specialist predators, the high reproductive performance achieved during years of high food abundance is essential to achieve high lifetime reproductive performance  and to maintain the whole population dynamics at large-scale . Our results indicate that maintaining these heterogeneous agricultural habitats (probably correlated with lower agricultural intensification), allowing kestrels to take full advantage of vole peak abundance are essential for the conservation of this farmland raptor and potentially many other species dependent of agro-ecosystems (e.g.; ). Our findings have important implications for biodiversity conservation in agricultural landscapes, since the loss of ecological heterogeneity at multiple spatial and temporal scales is a universal consequence of agricultural intensification and a key threat to biodiversity in farmland areas [118, 119].
Dhondt AA, Kempenaers B, Adriaensen F. Density-dependent clutch size caused by habitat heterogeneity. J Anim Ecol. 1992;61(3):643–8.
Arlt D, Pärt T. Nonideal breeding habitat selection: a mismatch between preference and fitness. Ecology. 2007;88(3):792–801.
Fretwell SD. Populations in a seasonal environment. Princeton, N.J: Princeton University Press; 1972.
Fretwell SD, Lucas HL. On territorial behavior and other factors influencing habitat distribution in birds. Acta Biotheor. 1969;19(1):16–36.
Sergio F, Blas J, Forero MG, Donzar JA, Hiraldo F. Sequential settlement and site dependence in a migratory raptor. Behav Ecol. 2007;18(5):811–21.
Verhulst S, Nilsson J-Å. The timing of birds' breeding seasons: a review of experiments that manipulated timing of breeding. Philosophical Transactions of the Royal Society B: Biological Sciences. 2008;363(1490):399–410.
Walther G-R, Post E, Convey P, Menzel A, Parmesan C, Beebee TJC, Fromentin J-M, Hoegh-Guldberg O, Bairlein F. Ecological responses to recent climate change. Nature. 2002;416(6879):389–95.
Sala OE, Stuart Chapin F III, Armesto JJ, Berlow E, Bloomfield J, Dirzo R, Huber-Sanwald E, Huenneke LF, Jackson RB, et al. Global biodiversity scenarios for the year 2100. Science. 2000;287(5459):1770–4.
Huhta E, Jokimäki J. Breeding occupancy and success of two hole-nesting passerines: the impact of fragmentation caused by forestry. Ecography. 2001;24(4):431–40.
Newbold T, Hudson LN, Hill SL, Contu S, Lysenko I, Senior RA, Börger L, Bennett DJ, Choimes A, Collen B. Global effects of land use on local terrestrial biodiversity. Nature. 2015;520(7545):45.
Battin J. When good animals love bad habitats: ecological traps and the conservation of animal populations. Conserv Biol. 2004;18(6):1482–91.
Grüebler MU, Schuler H, Müller M, Spaar R, Horch P, Naef-Daenzer B. Female biased mortality caused by anthropogenic nest loss contributes to population decline and adult sex ratio of a meadow bird. Biol Conserv. 2008;141(12):3040–9.
Bretagnolle V, Villers A, Denonfoux L, Cornulier T, Inchausti P, Badenhausser I. Rapid recovery of a depleted population of Little bustards Tetrax tetrax following provision of alfalfa through an Agri-environment scheme. Ibis. 2011;153(1):4–13.
Carrete M, Jose A, Sánchez-Zapata JL, Tella JM, Gil-Sánchez J, Moleón M. Components of breeding performance in two competing species: habitat heterogeneity, individual quality and density-dependence. Oikos. 2006;112(3):680–90.
Carrete M, Sánchez-Zapata J, Calvo JF, Lande R. Demography and habitat availability in territorial occupancy of two competing species. Oikos. 2005;108(1):125-136.
Almasi B, Béziers P, Roulin A, Jenni L. Agricultural land use and human presence around breeding sites increase stress-hormone levels and decrease body mass in barn owl nestlings. Oecologia. 2015;179:89–101.
Labocha MK, Hayes JP. Morphometric indices of body condition in birds: a review. J Ornithol. 2012;153(1):1–22.
Jönsson KI, Korpimäki E, Pen I, Tolonen P. Daily energy expenditure and short-term reproductive costs in free-ranging Eurasian kestrels (Falco tinnunculus). Funct Ecol. 1996;10(4):475–82.
Krone O, Waldenström J, Valkiūnas G, Lessow O, Müller K, Iezhova TA, Fickel J, Bensch S. Haemosporidian blood parasites in European birds of prey and owls. J Parasitol. 2008;94(3):709–15.
LaPointe DA, Atkinson CT, Samuel MD. Ecology and conservation biology of avian malaria. Ann N Y Acad Sci. 2012;1249(1):211–26.
Korpimäki E, Hakkarainen H, Bennett GF. Blood parasites and reproductive success of Tengmalm's owls: detrimental effects on females but not on males? Funct Ecol. 1993;7(4):420–6.
Wiehn J, Korpimäki E, Pen I. Haematozoan infections in the Eurasian kestrel: effects of fluctuating food supply and experimental manipulation of parental effort. Oikos. 1999;84(1):87–98.
Karell P, Ahola K, Karstinen T, Kolunen H, Siitari H, Brommer JE. Blood parasites mediate morph-specific maintenance costs in a colour polymorphic wild bird. J Evol Biol. 2011;24(8):1783–92.
Coltman DW, Slate J. Microsatellite measures of inbreeding: a meta-analysis. Evolution. 2003;57(5):971–83.
Chapman JR, Nakagawa S, Coltman DW, Slate J, Sheldon BC. A quantitative review of heterozygosity–fitness correlations in animal populations. Mol Ecol. 2009;18(13):2746–65.
Agudo R, Carrete M, Alcaide M, Rico C, Hiraldo F, Donázar JA. Genetic diversity at neutral and adaptive loci determines individual fitness in a long-lived territorial bird. Proc R Soc Lond B Biol Sci. 2012;279(1741):3241–9.
Wetzel DP, Stewart IRK, Westneat DF. Heterozygosity predicts clutch and egg size but not plasticity in a house sparrow population with no evidence of inbreeding. Mol Ecol. 2012;21(2):406–20.
Wetzel DP, Hatch MI, Westneat DF. Genetic sources of individual variation in parental care behavior. Behav Ecol Sociobiol. 2015;69(12):1933–43.
García-Navas V, Ortego J, Sanz JJ. Heterozygosity-based assortative mating in blue tits (Cyanistes caeruleus): implications for the evolution of mate choice. Proc R Soc B Biol Sci. 2009;276(1669):2931–40.
Sumasgutner P, Vasko V, Varjonen R, Korpimäki E. Public information revealed by pellets in nest sites is more important than ecto-parasite avoidance in the settlement decisions of Eurasian kestrels. Behav Ecol Sociobiol. 2014;68(12):2023–34.
Newton I, Rothery P. Senescence and reproductive value in sparrowhawks. Ecology. 1997;78(4):1000–8.
Murgatroyd M, Roos S, Evans R, Sansom A, Whitfield PDS, Reid R, Grant J, Amar A. Sex-specific patterns of reproductive senescence in a long-lived reintroduced raptor. J Anim Ecol. 2018;87(6):1587–99.
Donald PF, Green RE, Heath MF. Agricultural intensification and the collapse of Europe's farmland bird populations. Proc R Soc Lond Ser B Biol Sci. 2001;268(1462):25–9.
Butet A, Michel N, Rantier Y, Comor V, Hubert-Moy L, Nabucet J, Delettre Y. Responses of common buzzard (Buteo buteo) and Eurasian kestrel (Falco tinnunculus) to land use changes in agricultural landscapes of Western France. Agric Ecosyst Environ. 2010;138(3–4):152–9.
Inger R, Gregory R, Duffy JP, Stott I, Voříšek P, Gaston KJ. Common European birds are declining rapidly while less abundant species' numbers are rising. Ecol Lett. 2015;18(1):28–36.
Saurola P. Monitoring birds of prey in Finland: a summary of methods, trends, and statistical power. AMBIO: A Journal of the Human Environment. 2008;37(6):413–20.
Wallin K, Wallin M, Levin M, Järås T, Strandvik P. Leap-frog migration in the Swedish kestrel Falco tinnunculus population. Acta Regiae Societatis scientiarum et litterarum Gothoburgensis Zoologica. 1987;14:213–22.
Village A. The kestrel. London: T & AD Poyser; 1990.
Jost G, Weiler M, Gluns DR, Alila Y. The influence of forest and topography on snow accumulation and melt at the watershed-scale. J Hydrol. 2007;347(1):101–15.
Koivusalo H, Kokkonen T. Snow processes in a forest clearing and in a coniferous forest. J Hydrol. 2002;262(1):145–64.
López-Moreno IJ, Latron J. Spatial heterogeneity in snow water equivalent induced by forest canopy in a mixed beech–fir stand in the Pyrenees. Ann Glaciol. 2008;49:83–90.
Varhola A, Coops NC, Weiler M, Moore RD. Forest canopy effects on snow accumulation and ablation: an integrative review of empirical results. J Hydrol. 2010;392(3):219–33.
Korpimäki E. Diet of the kestrel Falco tinnunculus in the breeding season. Ornis Fenn. 1985;62:130–7.
Sonerud GA. Effect of snow cover on seasonal changes in diet, habitat, and regional distribution of raptors that prey on small mammals in boreal zones of Fennoscandia. Ecography. 1986;9(1):33–47.
Korpimäki E, Oksanen L, Oksanen T, Klemola T, Norrdahl KAI, Banks PB. Vole cycles and predation in temperate and boreal zones of Europe. J Anim Ecol. 2005;74(6):1150–9.
Kivinen S, Luoto M, Kuussaari M, Helenius J. Multi-species richness of boreal agricultural landscapes: effects of climate, biotope, soil and geographical location. J Biogeogr. 2006;33(5):862–75.
Vepsäläinen V, Tiainen J, Holopainen J, Piha M, Seimola T. Improvements in the Finnish Agri-environment scheme are needed in order to support rich farmland avifauna. Ann Zool Fenn. 2010;47(5):287–305.
Santangeli A, Lehikoinen A, Lindholm T, Herzon I. Organic animal farms increase farmland bird abundance in the boreal region. PLoS One. 2019;14(5):e0216009.
Korpimäki E. Diet variation, hunting habitat and reproductive output of the kestrel Falco tinnunculus in the light of the optimal diet theory. Ornis Fenn. 1986;63(3):84–90.
Korpimäki E, Wiehn J. Clutch size of kestrels: seasonal decline and experimental evidence for food limitation under fluctuating food conditions. Oikos. 1998;83(2):259–72.
Korpimäki E, Hakkarainen H. The boreal owl: ecology, behaviour and conservation of a forest-dwelling predator. Cambridge: Cambridge University press; 2012.
Morosinotto C, Villers A, Thomson RL, Varjonen R, Korpimäki E. Competitors and predators alter settlement patterns and reproductive success of an intraguild prey. Ecol Monogr. 2017;87:4–20.
Magurran AE: Measuring biological diversity: John Wiley & Sons; 2013.
Oksanen J, Blanchet FG, Kindt R, Legendre P, Minchin PR, O'Hara RB, Simpson GL, Solymos P, Stevens MHH, Wagner H: vegan: Community Ecology Package, version 2.2–1. vegan: Community Ecology Package 2015:version 2.2–1.
Navarro-López J, Fargallo JA. Trophic niche in a raptor species: the relationship between diet diversity, habitat diversity and territory quality. PLoS One. 2015;10(6):e0128855.
Petty SJ, Anderson DIK, Davison M, Little B, Sherratt TN, Thomas CJ, Lambin X. The decline of common kestrels Falco tinnunculus in a forested area of northern England: the role of predation by northern goshawks Accipiter gentilis. Ibis. 2003;145(3):472–83.
Suhonen J, Norrdahl K, Korpimaki E. Avian predation risk modifies breeding bird community on a farmland area. Ecology. 1994;75(6):1626–34.
Norrdahl K, Korpimäki E. Fear in farmlands: how much does predator avoidance affect bird community structure? J Avian Biol. 1998;29(1):79–85.
Hakkarainen H, Ilmonen P, Koivunen V, Korpimäki E. Experimental increase of predation risk induces breeding dispersal of Tengmalm's owl. Oecologia. 2001;126(3):355–9.
Hakkarainen H, Mykrä S, Kurki S, Korpimäki E, Nikula A, Koivunen V. Habitat composition as a determinant of reproductive success of Tengmalm's owls under fluctuating food conditions. Oikos. 2003;100(1):162–71.
Valkama J, Korpimäki E. Nestbox characteristics, habitat quality and reproductive success of Eurasian kestrels. Bird Study. 1999;46(1):81–8.
Treinys R, Bergmanis U, Väli Ü. Strong territoriality and weak density-dependent reproduction in lesser spotted Eagles Clanga pomarina. Ibis. 2017;159(1):343–51.
Hansson L, Henttonen H. Gradients in density variations of small rodents: the importance of latitude and snow cover. Oecologia. 1985;67(3):394–402.
Terraube J, Villers A, Poudré L, Varjonen R, Korpimäki E. Increased autumn rainfall disrupts predator–prey interactions in fragmented boreal forests. Glob Chang Biol. 2017;23(4):1361–73.
Valkama J, Korpimäki E, Wiehn J, Pakkanen T. Inter-clutch egg size variation in kestrels Falco tinnunculus: seasonal decline under fluctuating food conditions. J Avian Biol. 2002;33(4):426–32.
Henttonen H, Oksanen T, Jortikka A, Haukisalmi V. How much do weasels shape microtine cycles in the northern Fennoscandian taiga? Oikos. 1987;50(3):353–65.
Korpimäki E, Klemola T, Norrdahl K, Oksanen L, Oksanen T, Banks PB, Batzli GO, Henttonen H. Vole cycles and predation. Trends Ecol Evol. 2003;18(10):494–5.
Huitu O, Norrdahl K, Korpimäki E. Landscape effects on temporal and spatial properties of vole population fluctuations. Oecologia. 2003;135(2):209–20.
Huitu O, Laaksonen J, Klemola T, Korpimäki E. Spatial dynamics of Microtus vole populations in continuous and fragmented agricultural landscapes. Oecologia. 2008;155(1):53–61.
Terraube J, Villers A, Ruffino L, Iso-Iivari L, Henttonen H, Oksanen T, Korpimäki E. Coping with fast climate change in northern ecosystems: mechanisms underlying the population-level response of a specialist avian predator. Ecography. 2015;38(7):690–9.
Vasko V, Laaksonen T, Valkama J, Korpimäki E. Breeding dispersal of Eurasian kestrels Falco tinnunculus under temporally fluctuating food abundance. J Avian Biol. 2011;42(6):552–63.
Kostrzewa R, Kostrzewa A. Zur Jugendentwicklung des Turmfalken (Falco tinnunculus) ein Altersbestimmungsschlüssel. Ökologie der Vögel. 1987;9:119–25.
van Paassen AG, Veldman DH, Beintema AJ. A simple device for determination of incubation stages in eggs. Wildfowl. 1984;35(35):173–8.
Liebezeit JR, Smith PA, Lanctot RB, Schekkerman H, Tulp I, Kendall SJ, Tracy DM, Rodrigues RJ, Meltofte H, Robinson JA. Assessing the development of shorebird eggs using the flotation method: species-specific and generalized regression models. Condor. 2007;109(1):32–47.
Ackerman JT, Eagles-Smith CA. Accuracy of egg flotation throughout incubation to determine embryo age and incubation day in waterbird nests. Condor. 2010;112(3):438–46.
Village A, Marquiss M, Cook DC. Moult, ageing and sexing of kestrels. Ringing Migr. 1980;3(2):53–9.
Forsman D. Rovfågelsguiden. Helsinki: Lintutieto Oy; 1984.
Schulte-Hostedde AI, Zinner B, Millar JS, Hickling GJ. Restitution of mass–size residuals: validating body condition indice. Ecology. 2005;86(1):155–63.
Massemin S, Korpimäki E, Wiehn J. Reversed sexual size dimorphism in raptors: evaluation of the hypotheses in kestrels breeding in a temporally changing environment. Oecologia. 2000;124(1):26–32.
Terraube J, Vasko V, Korpimäki E. Mechanisms and reproductive consequences of breeding dispersal in a specialist predator under temporally varying food conditions. Oikos. 2015;124:762–71.
Valkiūnas G: Avian malaria parasites and other haemosporidia: CRC press; 2004.
Nesje M, Røed KH, Lifjeld JT, Lindberg P, Steen OF. Genetic relationships in the peregrine falcon (Falco peregrinus) analysed by microsatellite DNA markers. Mol Ecol. 2000;9(1):53–60.
Padilla J, Parejo J, Salazar J, Martínez-Trancón M, Rabasco A, Sansinforiano E, Quesada A. Isolation and characterization of polymorphic microsatellite markers in lesser kestrel (Falco naumanni) and cross-amplification in common kestrel (Falco tinnunculus). Conserv Genet. 2009;10(5):1357–60.
Ortego J, González EG, Sánchez-Barbudo I, Aparicio JM, Cordero PJ. Novel highly polymorphic loci and cross-amplified microsatellites for the lesser kestrel Falco naumanni. Ardeola. 2007;54(1):101–8.
Coulon A. Genhet: an easy-to-use R function to estimate individual heterozygosity. Mol Ecol Resour. 2010;10(1):167–9.
Selonen V, Hanski IK. Condition-dependent, phenotype-dependent and genetic-dependent factors in the natal dispersal of a solitary rodent. J Anim Ecol. 2010;79(5):1093–100.
Vanpé C, Debeffe L, Hewison AJM, Quéméré E, Lemaître J-F, Galan M, Amblard B, Klein F, Cargnelutti B, Capron G, et al. Reduced microsatellite heterozygosity does not affect natal dispersal in three contrasting roe deer populations. Oecologia. 2015;177(3):631–43.
Bates D, Maechler M, Bolker B, Walker S: lme4: Linear mixed-effects models using Eigen and S4. R package version 1.1–7. In.; 2014.
Barton K: MuMIn: Multi-model inference. R package version 1.10.5. In: Model selection and model averaging based on information criteria (AICc and alike). 2014.
Grueber CE, Nakagawa S, Laws RJ, Jamieson IG. Multimodel inference in ecology and evolution: challenges and solutions. J Evol Biol. 2011;24(4):699–711.
Grueber CE, Nakagawa S, Laws RJ, Jamieson IG. Corrigendum to "multimodel inference in ecology and evolution: challenges and solutions". J Evol Biol. 2011;24(7):1627–7.
Symonds M, Moussalli A. A brief guide to model selection, multimodel inference and model averaging in behavioural ecology using Akaike's information criterion. Behav Ecol Sociobiol. 2011;65:13–21.
Sumasgutner P, Nemeth E, Tebb G, Krenn HW, Gamauf A. Hard times in the city - attractive nest sites but insufficient food supply lead to low reproduction rates in a bird of prey. Front Zool. 2014;11:48.
Nakagawa S, Freckleton R. Model averaging, missing data and multiple imputation: a case study for behavioural ecology. Behav Ecol Sociobiol. 2011;65(1):103–16.
Schielzeth H. Simple means to improve the interpretability of regression coefficients. Methods Ecol Evol. 2010;1(2):103–13.
Zuur AF, Ieno EE, Walker NJ, Saveliev AA, Smith GM. Mixed effects models and extensions in ecology with R. New York, NY: Springer; 2009.
Mazerolle M: AICcmodavg: Model selection and multimodel inference based on (Q)AIC(c). R package version 2.0–1. In.; 2014.
Burnham KP, Anderson DR. Model selection and multimodel inference: a practical information-theoretic approach. 2nd ed. New York: Springer; 2002.
Anderson DR, Link WA, Johnson DH, Burnham KP. Suggestions for presenting the results of data analyses. J Wildl Manag. 2001;65(3):373–8.
Giam X, Olden JD. Quantifying variable importance in a multimodel inference framework. Methods Ecol Evol. 2016;7(4):388–97.
Lenth R: Estimated Marginal Means, aka Least-Squares Means. R package version 134 2019.
Development Core Team R. R: a language and environment for statistical computing. R version 3.4.4. In. Vienna: R Foundation for Statistical Computing; 2018.
Paradis E, Claude J, Strimmer K. APE: analyses of phylogenetics and evolution in R language. Bioinformatics. 2004;20:289–90.
Pebesma EJ. Multivariable geostatistics in S: the gstat package. Comput Geosci. 2004;30(7):683–91.
Cam E, Aubry L. Early development, recruitment and life history trajectory in long-lived birds. J Ornithol. 2011;152:187–201.
Daan S, Dijkstra C, Tinbergen JM. Family planning in the kestrel (Falco tinnunculus): the ultimate control of covariation of laying date and clutch size. Behaviour. 1990;114(1/4):83–116.
Alain B, Gilles P, Yannick D. Factors driving small rodents assemblages from field boundaries in agricultural landscapes of Western France. Landsc Ecol. 2006;21(3):449–61.
Korpimäki E. Prey choice strategies of the kestrel Falco tinnunculus in relation to available small mammals and other Finnish birds of prey. Ann Zool Fenn. 1985;22(1):91–104.
Terraube J, Arroyo BE, Bragin A, Bragin E, Mougeot F. Ecological factors influencing the breeding distribution and success of a nomadic, specialist predator. Biodivers Conserv. 2012;21(7):1835–52.
Kokko H, Sutherland WJ. Ecological traps in changing environments: ecological and evolutionary consequences of a behaviourally mediated Allee effect. Evol Ecol Res. 2001;3(5):537–51.
Kristan WB. The role of habitat selection behavior in population dynamics: source–sink systems and ecological traps. Oikos. 2003;103(3):457–68.
Robertson BA, Hutto RL. A framework for understanding ecological traps and an evaluation of existing evidence. Ecology. 2006;87(5):1075–85.
Schlaepfer MA, Runge MC, Sherman PW. Ecological and evolutionary traps. Trends in Ecology and Evolution. 2002;17(10):474–80.
Sergio F, Tanferna A, De Stephanis R, Jiménez LL, Blas J, Tavecchia G, Preatoni D, Hiraldo F. Individual improvements and selective mortality shape lifelong migratory performance. Nature. 2014;515:410.
Korpimäki E. Fluctuating food abundance determines the lifetime reproductive success of male Tengmalm's owls. J Anim Ecol. 1992:103–11.
Terraube J, Bretagnolle V. Top-down limitation of mesopredators by avian top predators: a call for research on cascading effects at the community and ecosystem scale. Ibis. 2018;160(3):693–702.
Smallwood JA, Causey MF, Mossop DH, Klucsarits JR, Robertson B, Robertson S, Mason J, Maurer MJ, Melvin RJ, Dawson RD, et al. Why are American kestrel (Falco sparverius) populations declining in North America? Evidence from nest-box programs. J Raptor Res. 2009;43(4):274–82.
Benton TG, Vickery JA, Wilson JD. Farmland biodiversity: is habitat heterogeneity the key? Trends Ecol Evol. 2003;18(4):182–8.
Piha M, Tiainen J, Holopainen J, Vepsäläinen V. Effects of land-use and landscape characteristics on avian diversity and abundance in a boreal agricultural landscape with organic and conventional farms. Biol Conserv. 2007;140(1–2):50–61.
We wish to thank Brigitte Planade, Rauno Varjonen, Ville Vasko and Jorma Nurmi for their great help during field work, and Meri Lindqvist, Katja Salminen, Elisabeth Haring, Barbara Däubl, Johannes Rath and Eva Ringler for their assistance during lab work, optimisation of the protocols and troubleshooting peak scans. We would like to acknowledge the valuable contribution of the Adlerarena Burg Landskron, Austria, specifically Franz Schüttelkopf, for their contribution of kestrel blood samples to test microsatellites beforehand on individuals of known relatedness. We wish to thank Bernd Gruber and Aaron Adamack for their repeated advice and patience in getting R codes right to use their package ‘PopGenReport’, as well as Staffan Roos and three anonymous reviewers for valuable comments on previous versions of the manuscript.
The project was financially supported by the Academy of Finland (grant no. 123379, 136717 and 250709 to EK). The genetic analyses were funded by the Environmental Protection Bureau of Vienna (MA 22–2220/2010), the Vienna Science and Technology Fund (H-2483/2012) and the research grant of the German Ornithologists´ Society (DO-G); all granted to PS. PS was financially supported by the Marietta Blau Grant of the Austrian Centre for International Cooperation and Mobility and the Finnish Cultural Foundation (grant no. H91042). NC was supported by a Marie Curie Grant (PIEF-GA-2013-625883).
Ethics approval and consent to participate
Bleeding and trapping of kestrels was executed in accordance to Finnish and EU Laws and regulations and under the approval of the animal experiment committee (permit number ESAVI-2010-05480/Ym-23 and ringing license no. 524 to EK), and followed the Weatherall Report and the guidelines for the treatment of animals in behavioural research and teaching (ASAB 2012).
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Table S1. Table of habitats found in the study area and Eurasian kestrel territories. Figure S2. Figure showing the relationship between territory land cover heterogeneity and percentage of farmland area in kestrel territories. Figure S3. Figure showing the periodic 3-year vole cycle over the entire long-term survey period from 1973 to 2015. S4 Material and Methods. Blood parasite infection. Table S5. Table of Primer set Falconidae: Repeat motif and primer sequences for 24 microsatellites. S6 Material and Methods. Individual genetic heterozygosity. Table S7. Candidate list for parameters influencing the timing of breeding (Julian day of egg-laying) and nestling survival (ratio of eggs that fledged successfully) in Eurasian kestrels; and complete model list following AICc ranking and model weights. Figure S8. Figure of spatial distribution of ‘individual quality’ indices quantified in male and female kestrels. Figure S9. Figure of least square means post-doc contrasts of the interaction term age of the breeding adult, and the vole cycle, influencing nestling survival in Eurasian kestrels. (PDF 508 kb)
Supporting data. (XLSX 82 kb)
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Sumasgutner, P., Terraube, J., Coulon, A. et al. Landscape homogenization due to agricultural intensification disrupts the relationship between reproductive success and main prey abundance in an avian predator. Front Zool 16, 31 (2019). https://doi.org/10.1186/s12983-019-0331-z
- Biodiversity conservation
- Boreal landscapes
- Eurasian kestrel
- Global change
- Heterozygosity–fitness correlations
- Individual quality
- Predator-prey interactions