Fig. 1

Functional analysis of M. inceptum AOX expressed in S. cerevisiae mitochondria. Tardigrade AOX expression was induced by the addition of galactose to YPG medium. (a) and (b) Representative traces of the performed measurements of the rate of oxygen uptake by intact yeast cells in the absence (AOX not expressed) and in the presence (AOX expressed) of galactose. (c) Changes in S. cerevisiae cell respiration in the presence or the absence of AOX expression and after addition of inhibitors of AOX (BHAM) and MRC complex IV (KCN). The applied concentrations of inhibitors were as follows: 1 mM KCN and 3 mM BHAM. ** p < 0.01; *** p < 0.001; n/s not statistically significant. (d) Detection of M. inceptum AOX expression and maintenance in S. cerevisiae mitochondria in the presence of galactose. Mitochondria were isolated from cells cultured in the absence of galactose (control) and in its presence, after 2 and 24 h. The red arrows indicate bands corresponding to M. inceptum AOX