Expression profiles of NAGs and CHTs upregulated in the pleuropodia of S. gregaria across development. Top row: NAGs, bottom row: CHTs. (a)-(d) and (e)-(h): RNA-seq; expression in single-sample sequencing is shown. (b') and (e'): real-time RT-PCR. (b') is the same gene as in (b) and (e') is the same gene as in (e). Analysis of 3–4 technical replicates is shown. Expression in day 8 was set as 1. Values are mean ± s.d.