Gene expression profiling of whole blood cells supports a more efficient mitochondrial respiration in hypoxia-challenged gilthead sea bream (Sparus aurata)
© The Author(s). 2017
Received: 4 April 2017
Accepted: 28 June 2017
Published: 6 July 2017
Acclimation to abiotic challenges, including decreases in O2 availability, requires physiological and anatomical phenotyping to accommodate the organism to the environmental conditions. The retention of a nucleus and functional mitochondria in mature fish red blood cells makes blood a promising tissue to analyse the transcriptome and metabolic responses of hypoxia-challenged fish in an integrative and non-invasive manner.
Juvenile gilthead sea bream (Sparus aurata) were reared at 20–21 °C under normoxic conditions (> 85% O2 saturation) followed by exposure to a gradual decrease in water O2 concentration to 3.0 ppm (41–42% O2 saturation) for 24 h or 1.3 ppm (18–19% O2 saturation) for up to 4 h. Blood samples were collected at three different sampling points for haematological, biochemical and transcriptomic analysis.
Blood physiological hallmarks remained almost unaltered at 3.0 ppm, but the haematocrit and circulating levels of haemoglobin, glucose and lactate were consistently increased when fish were maintained below the limiting oxygen saturation at 1.3 ppm. These findings were concurrent with an increase in total plasma antioxidant activity and plasma cortisol levels, whereas the opposite trend was observed for growth-promoting factors, such as insulin-like growth factor I. Additionally, gene expression profiling of whole blood cells revealed changes in upstream master regulators of mitochondria (pgcβ and nrf1), antioxidant enzymes (gpx1, gst3, and sod2), outer and inner membrane translocases (tom70, tom22, tim44, tim10, and tim9), components of the mitochondrial dynamics system (mfn2, miffb, miro1a, and miro2), apoptotic factors (aifm1), uncoupling proteins (ucp2) and oxidative enzymes of fatty acid β-oxidation (acca2, ech, and hadh), the tricarboxylic acid cycle (cs) and the oxidative phosphorylation pathway. The overall response is an extensive reduction in gene expression of almost all respiratory chain enzyme subunits of the five complexes, although mitochondrial-encoded catalytic subunits and nuclear-encoded regulatory subunits of Complex IV were primarily increased in hypoxic fish.
Our results demonstrate the re-adjustment of mitochondrial machinery at transcriptional level to cope with a decreased basal metabolic rate, consistent with a low risk of oxidative stress, diminished aerobic ATP production and higher O2-carrying capacity. Taken together, these results suggest that whole blood cells can be used as a highly informative target tissue of metabolic condition.
KeywordsBlood transcriptomics Hypoxia Limiting oxygen saturation Mitochondrial activity Oxphos Sparus aurata
Among the abiotic factors, dissolved oxygen (O2) is particularly important as the major limiting factor of fish aerobic metabolism [1, 2]. When regulatory mechanisms are no longer sufficient to maintain the O2 consumption rate (MO2), further reductions in MO2 occur at a certain level of O2 saturation . This threshold is termed the limiting oxygen saturation (LOS) in fed fish able to maintain a routine metabolic rate, and according to the oxystatic control theory of feed intake, fish adjust their feed intake to meet dietary O2 demands . Therefore, changes in LOS, produced by fluctuations in O2 solubility associated with variations in water temperature, should be considered and regulated to ensure a non-compromised physiological function and guarantee the welfare of farmed fish fed high or low O2-demanding diets [5, 6]. This regulation is mediated through O2 sensors that trigger anaerobic metabolic rates to compensate for the decreasing aerobic ATP production [7, 8]. For this purpose, eukaryotic cells switch from mitochondrial oxidative phosphorylation (OXPHOS) to the less efficient anaerobic glycolytic pathway, which induces stress and lactic acidosis (reviewed in ). The hallmarks of human muscle adaptation to hypoxia are a decrease in muscle oxidative capacity concomitant with a decrease in aerobic work capacity [10, 11]. In this regard, hypo-metabolic states should be considered as part of the adaptive response to hypoxia instead of a negative result in hypoxia-tolerant individuals  since this metabolic depression prevents the accumulation of toxic by-products from anaerobic metabolism .
In fish, microarray gene expression profiling of liver and skeletal muscle demonstrated that metabolic suppression is a key adaptive strategy in the hypoxic goby fish, Gillichthys mirabilis, to drive energy resources from growth towards metabolic processes that are essential for hypoxia survival . However, in Fundulus grandis, both cardiac and hepatic tissues displayed increases in the gene expression of different enzyme subunits of the OXPHOS pathway in response to short-term hypoxic exposure . Similarly, confounding results have been reported in European sea bass (Dicentrarchus labrax), as early life exposure to moderate hypoxia has long-lasting detrimental effects on growth performance with no improvement of hypoxia tolerance in juvenile fish despite the enhanced expression of glycolytic enzymes, which are target genes of hypoxia-inducible factors . Whether this response is tissue- or fish species-specific remains unclear. Importantly, the red blood cells (RBC) of fish and almost all amphibians, reptiles and birds retain a nucleus and functional mitochondria . These RBCs present new research opportunities, and previous research attempts have demonstrated that the expression of mitochondrial uncoupling proteins is highly regulated by hypoxia stimuli in the whole blood cells of gilthead sea bream (Sparus aurata) . Certainly, fish microarray meta-analysis revealed that mitochondria are particularly sensitive to cellular stress triggered by a wide range of nutritional and environmental stress stimuli . Hence, a PCR-array containing 88 mitochondrial-related markers has been useful to examine changes in hepatic and muscle metabolism in response to short-term fasting  or aquaculture stressors that mimic thermal stress and daily operational farming activities in gilthead sea bream . There is little information on blood transcriptomics, and the aim of the present study was to provide new insights into the regulation and adaptive responses of hypoxic metabolism in fish, combining non-invasive transcriptional approaches based on mitochondrial markers with conventional measures of blood haematology and biochemistry. This type of approach is crucial to determine whether samples collected without sacrificing animals provide a reliable measure of mitochondrial functioning and energy metabolism at the level of the whole organism.
Juvenile gilthead sea bream of Atlantic origin (Ferme Marine du Douhet, Bordeaux, France) were reared from early life stages at the indoor experimental facilities of the Institute of Aquaculture Torre de la Sal (IATS-CSIC, Castellón, Spain) under natural photoperiod and temperature conditions at our latitude (40°5′N; 0°10′E). Seawater was pumped ashore (open system) and filtered through a 10-μm filter. The O2 content of water effluents under standard conditions remained consistently higher than 85% saturation, and unionised ammonia under both control and experimental conditions remained below toxic levels (<0.02 mg/L). For sampling, the fish were anaesthetised using 3-aminobenzoic acid ethyl ester (100 mg/L), and blood was drawn from caudal vessels using EDTA-treated syringes.
Experimental setup and sampling
In each test, normoxic or hypoxia-challenged fish were sampled at three different sampling points after decreasing the water O2 concentration (8 fish per time and condition): i) H1: T0, T1 (24 h), T2 (48 h), and ii) H2: T0, T1 (7 h), T2 (11 h). One blood aliquot (150 μL) was directly collected into a microtube containing 500 μL of stabilising lysis solution (REAL Total RNA Spin Blood Kit, Durviz, Valencia, Spain) and stored at −80 °C until total RNA extraction. Other aliquots were processed for haematocrit (Hc) and haemoglobin (Hb) determinations. The remaining blood was centrifuged at 3000 × g for 20 min at 4 °C, and the plasma samples were frozen and stored at −20 °C until biochemical and hormonal analyses were performed.
Blood biochemistry and hormonal parameters
Hc was measured using heparinised capillary tubes centrifuged at 1500 × g for 30 min in a Sigma 1–14 centrifuge (Sigma, Osterode am Harz, Germany). The Hb concentration was assessed using a Hemocue Hb 201+ (Hemocue, Ängelholm, Sweden). Plasma glucose was analysed using the glucose oxidase method (Thermo Electron, Louisville, CO, USA). Blood lactate was measured in deproteinised samples (perchloric acid 8%) using an enzymatic method based on the use of lactate dehydrogenase (Instruchemie, Delfzijl, The Netherlands). Total antioxidant capacity in plasma samples was measured using a commercial kit (Cayman Chemical, Ann Arbor, MI, USA) adapted to 96-well microplates. This assay relies on the ability of the antioxidants in the samples to inhibit the oxidation of ABTS (2,2′-azino-di-[3-ethylbenzothiazoline sulphonate]) to the ABTS radical cation by metamyoglobin, a derivatised form of myoglobin. The capacity of the sample to prevent ABTS oxidation was compared with that of Trolox (water-soluble tocopherol analogue) and quantified as mM Trolox equivalents. Plasma cortisol levels were analysed using an EIA kit (Kit RE52061 m IBL, International GmbH, Germany). The limit of detection of the assay was 2.46 ng/mL with intra- and inter-assay coefficients of variation lower that 3% and 5%, respectively. Plasma insulin-like growth factors (Igf) were extracted using acid-ethanol cryoprecipitation , and the concentration was measured using a generic fish Igf-I RIA validated for Mediterranean perciform fish . The sensitivity and midrange of the assay were 0.05 and 0.7–0.8 ng/mL, respectively.
Gene expression analysis
This manuscript follows the ZFIN Zebrafish Nomenclature Guidelines for gene and protein names and symbols (https://wiki.zfin.org/display/general/ZFIN+Zebrafish+Nomenclature+Guidelines).
The data on biochemical and hormonal parameters were analysed using two-way analysis of variance (ANOVA), followed by the Holm-Sidak test. The data on gene expression were analysed using Student’s t test. The significance level was set at P < 0.05. All analyses were performed using SigmaPlot Version 13 for Windows.
Hypoxic effects on blood haematology and biochemistry
Hypoxic effects in whole blood cell gene expression profiling
Based on the results of hormonal and metabolic parameters, gene expression profiling of whole blood cells was restricted to the last sampling point of the severe hypoxia experiment (H2). The relative gene expression and fold-changes (FC) of mitochondrial-related genes are summarised in Table 1. For easier interpretation and visualisation of the results, the FC of differentially expressed genes is indicated using square symbols in red (up-regulated) or green (down-regulated). With the exception of pgc1α, all the genes included in the array were detected in all samples analysed. Among these genes, 41 out of 84 were differentially expressed, and the overall response involved repressed expression in response to severe hypoxia. This response was mediated by antioxidant enzymes (gpx1, gst3, and sod2), the transcription factor nrf1, outer and inner membrane translocases (tom70, tom22, tim44, tim10, and tim9), markers of mitochondrial dynamics and apoptosis (mfn2, miffb, miro1a, miro2, and aifm1), fatty acid β-oxidation (acaa2 and hadh), tricarboxylic acid cycle (cs), respiration uncoupling (ucp2) and respiratory enzyme subunits of Complex I (ndufa3, ndufa4, ndufa7, ndufb5, and ndufs7), Complex II (sdha, sdhaf1, and sdhaf2), Complex III (uqcrc1, uqcrc2, and uqcrh) and Complex V (atp5c1, atp5g1, atp5l, and atpaf2), encoded by either mitochondrial or nuclear DNA. The nuclear-encoded assembly factors of Complex IV (sco1, surf1, and cox15) were also significantly down-regulated, but the opposite trend was observed for catalytic (coxi) and regulatory (cox5a2 and cox8b) enzyme subunits of mitochondrial or nuclear origin, respectively. This up-regulation was also observed for the transcription factor pgc1ß and the outer membrane translocase tom34. The molecular chaperones were the only factors that did not significantly change under hypoxic conditions, although the overall trend was a down-regulation in hypoxic fish.
Studies in gilthead sea bream have indicated that the response of this species to a progressive decline in O2 concentration is to reduce its swimming activity, indicative of an increasing metabolic stress and/or a coping strategy to prolong survival time when hypoxia cannot be avoided . In the same study, the threshold level of LOS determined in 400-g fish varied from 17% O2 saturation at 12 °C and 36% O2 saturation at 20 °C. These O2 concentrations can be implemented in aquaculture as a lower limit for acceptable decreases in O2 concentration with respect to the physiological function and welfare of farmed gilthead sea bream. Therefore, as further explained below, it is not surprising that data on blood biochemistry and haematology in fish exposed to O2 concentrations above the LOS did not significantly vary after 24 h of hypoxia challenge. In contrast, a consistent response, exacerbated over time, was observed for blood parameters measured a few hours after exposure to O2 concentrations below the LOS. In this case, the gene expression profile of whole blood cells was analysed, and the molecular signatures of hypoxic fish revealed important changes consistent with reduced but more efficient aerobic ATP production.
Living organisms are characterised by continuous switching between resting and active states, which includes long resting periods with low ATP production . Similarly, the response to hypoxia has two main aspects characterised by defence and rescue, where the early defence stage is achieved by reducing energy needs (hypo-metabolic state) and the dependence on aerobic metabolism . In the case of gilthead sea bream, the antioxidant defences in fish fed diets supplemented with methionine and white tea were insufficient to avoid oxidative stress under moderate hypoxia induced by 40% O2 saturation at 22–23 °C . However, LOS increases with decreasing temperature , and the results of the present study showed that all measured haematological and biochemical parameters remained mostly unaltered in fish maintained at 20–21 °C and 41–42% O2 saturation. In contrast, a pronounced increase in Hc, Hb and plasma glucose and lactate levels was reported after exposure to severe hypoxia (18–19% O2 saturation) for 4 h under steady-state conditions. Indeed, this rapid response could reflect an increase in blood O2-carrying capacity  associated in the short term with erythrocyte release from a storage organ or with a reduction in plasma volume rather than the formation of new Hb . Consistent with , this finding likely reflects metabolic changes mediated by O2 sensors that drive the shift of the redox cellular status of NADH to a more reduced form with a rapid recycling of NAD+ to NADH. Certainly, hypoxic situations must improve and adjust the metabolic and O2-carrying capacities of challenged fish to cope and reach internal homeostasis . The trigger observed in plasma antioxidant capacity after acute and severe hypoxia demonstrates a general decrease in metabolic rates that also reflects the aerobic/anaerobic shift of metabolism [25, 30, 31].
The increase in plasma cortisol levels observed after severe hypoxia indicates a stressful scenario in the experimental model used in the present study. Other common features of hypoxic and stress conditions include a decrease in plasma Igf-I levels and concomitant growth inhibition [32, 33]. In this sense, a characteristic response in challenged gilthead sea bream produced by crowding, and presumably also through hypoxia, is the overall down-regulated expression of hepatic igfs and growth hormone receptors . Studies in rodents support the involvement of the Gh/Igf system in the regulation of key antioxidant enzymes, ROS production and scavenging as well mitochondrial biogenesis and activity [35, 36]. However, thus far, the precise mechanisms underlying these Gh/Igf-mediated effects remain unexplored in fish. Moreover, confounding results have been reported for the aerobic/anaerobic shift during hypoxia exposure, although studies in the euryoxic mudsucker Gillichthys mirabilis showed a tissue-specific gene regulation resulting in suppressed protein synthesis in skeletal muscle and enhanced anaerobic ATP production in the liver tissue . Similarly, zebra fish (Danio rerio) embryos survive during severe hypoxia (0–5% O2 saturation) through changes in the gene and protein expression of master regulators of O2 homeostasis, such as the hypoxia-inducible factor 1 alpha (hif-1α/Hif-1α) [37–40]. Additionally, long-term adaptive responses in the gene expression of several pathways related to cell architecture, cell division and energy metabolism have been underlined in the gills of adult hypoxic fish . In our experimental model, this hypothesis was perfectly consistent with hypoxic-mediated effects on mitochondrial-related markers of blood cells (see below).
Most mitochondrial proteins are encoded by nuclear DNA; thus, a healthy metabolic mitochondria phenotype is highly dependent on the protein import system, which involves two assembly complexes: the translocases of the outer membrane (TOM complex) and the translocases of the inner membrane (TIM complex) (see [42, 43] for review). Thus, as demonstrated in mammalian cells , the TOM/TIM complex is highly inducible and regulated at both transcriptional and post-transcriptional levels under conditions of chronic stress or energy deficit to ensure the maintenance of adequate mitochondrial protein import rates. Similarly, juveniles of gilthead sea bream exhibit a clear up-regulation in the gene expression of hepatic protein subunits of the TOM/TIM complex in response to aerobic energy stimuli after exposure to cyclic decreases in water temperature . Conversely, the present study demonstrated that severe hypoxia induced a pronounced down-regulation of tom70 and tom22 subunits in whole blood cells concurrent with decreases in mRNAs encoding protein subunits of TIM23 (tim44) and TIM22 (tim10 and tim9) complexes. In addition, co-expression analyses revealed the up-regulation of tom34, which acts as a co-chaperone of the Hsp70/Hsp90 complex, inhibiting mitochondrial protein translocation when expressed in excess . Taken together, these findings suggest in hypoxic fish an orchestration of the TOM/TIM complex that could enable adjustments in mitochondrial protein translocation to reduce plasma oxidative capacity and the risk of oxidative stress, a feature that is consistent with the down-regulated expression of markers of ROS production and scavenging, including ucp2, mitochondrial superoxide dismutase (sod2), enzymes of the glutathione system (gpx1 and gst3) and enzymes of fatty acid β-oxidation and TCA (acaa2, hadh, and cs). Importantly, the same trend was observed for mitochondrial (hsp10, dnaja3a, dnajc20, hsp60, and grp-75) and endoplasmic reticulum (grp-170) molecular chaperones, suggesting that proper protein folding was primarily assured in the blood cells of gilthead sea bream under the depressed metabolism induced by hypoxia exposure. Similarly, severe hypoxia did not induce the gene expression of heat shock proteins in rainbow trout (Oncorhynchus mykiss) RBCs cultured in vitro when the hypoxia challenge was not accompanied by a heat shock treatment .
Mitochondrial dynamics is an essential process that adapts mitochondria morphology to the bioenergetics requirements of the cell (see  for review). The mechanism of this biological process involves the balance of two opposing procedures (fusion and fission), but it is also greatly affected by the “railways” used by the mitochondria to move inside the cells. The functionality of these organelles favours the redistribution of mitochondria within the cell to ensure high oxidative capacity under conditions of high energy demand, enabling the removal of dysfunctional or damaged mitochondria. This mechanism is highly conserved from yeast to mammals , and the molecular identity of major components of the fusion (mfn1 and mfn2) and fission (fis1 and miffb) system, as well as those of the MIRO system (miro1a and miro2) has been characterised in gilthead sea bream and uploaded to public database repositories . Nevertheless, experimental evidences demonstrated that the gene expression of some of these effectors is highly induced in response to aerobic stimuli after cold-water exposure. In contrast, in the present study, severe hypoxia significantly repressed the expression of most components of this biological process (mfn2, miffb, miro1a, and miro2), including the well-known mitochondrial apoptotic factor aifm1. Consistently, the knockout of the transcriptional regulator pgc1ß is associated with a selective reduction in the expression in mice . The lack of Pgc1ß also impaired the thermogenic response of adipose tissue and hepatic lipid metabolism in response to high fat dietary loads . Therefore, Pgc1ß is essential for proper metabolic tuning in stress situations, contributing to the maintenance of the basal expression of mitochondrial and metabolic-related genes. However, in the present experimental model, the opposite regulation was observed for pgc1ß and mfn2, suggesting that the up-regulated expression of pgc1ß was more a consequence than the cause of the overall repressed expression of mitochondria-related genes. This notion was supported by the observation that the mitochondrial transcription factor nrf1, another target gene of pgc1ß , was also down-regulated in hypoxia-challenged fish. Notably, despite the overlapping gene expression of pgc1ß and its homologue pgc1α, the compensation of Pgc1α or Pgc1ß functions was not completely observed in Pgc1α or Pgc1ß knockout rodents [51–53]. In the case of gilthead sea bream blood cells, this effect is more exacerbated because pgc1α mRNAs were almost undetectable in both normoxic and hypoxic fish, although the expression of this gene at noticeable levels has previously been reported in other tissues of this fish species . Whether this effect is part of the evolutionary pressure to select the conservation of functional mitochondria in the nucleated RBCs of non-mammalian vertebrates remains to be established .
The ultimate effector for coping with changes in energy needs and aerobic ATP production is the regulation of the OXPHOS pathway, which comprises five enzyme complexes (I-V) with catalytic enzymatic subunits encoded by both nuclear or mitochondrial DNA, whereas the enzyme subunits with regulatory or assembly properties are strictly of nuclear origin . Changes in the enzymatic activities of the OXPHOS pathway have been studied for many years both in mammals and fish (e.g., [54–56]). Little is known at the molecular level, although gene expression profiling of liver, skeletal muscle and cardiac muscle tissues revealed that both the direction and magnitude of change is highly dependent on the metabolic capabilities of each tissue . Thus far, the molecular fingerprinting of the OXPHOS pathway remains primarily unexplored in blood cells, and this is the first to address the specific regulation of this pathway in response to environmental stressors, evidenced by the general depletion of several components of Complexes I, II, III and V in response to severe hypoxic stimuli. Assembly factors of Complex IV (sco1, surf1 and cox15) were also down-regulated in the present experimental model. These enzyme subunits play an important role in energy production, and mutations or defects in these molecules produce adverse effects in the appropriate function of the OXPHOS pathway in mammals [57–61]. However, this observation contrasted with the overall overexpression of catalytic and regulatory subunits of Complex IV, which was statistically significant for the catalytic coxi and the regulatory cox5a2 and cox8b subunits. CoxI protein is encoded by mitochondrial DNA and represents one of the largest subunits of Complex IV, which contains the bimetallic centre where O2 binds and is reduced to H2O [62, 63]. In addition, the observed increase in the gene expression of Cox5a and Cox8 family subunits highlights their importance during the completion of the holocomplex monomer, which contains the functional structure of the cytochrome c binding site (see  for review). Therefore, we hypothesised that the net effect should be a reduced mitochondrial ATP production due to the overall suppression of mRNAs encoding the enzyme subunits of Complexes I, II, III and IV, although the opposite regulation of the catalytic/regulatory components of Complex IV should be accompanied by subsequent mechanisms that allow a better exploitation of available oxygen in the most energetically favorable way. Modifications in mitochondrial properties also occur in other vertebrates, and the hypo-metabolic steady-state observed in overwintering frogs (Rana temporaria) occurred during hypoxic submergence by increases in mitochondrial O2 affinity and a reduction in resting (state 4) and active (state 3) respiration rates in mitochondria isolated from skeletal muscle . Similarly, early studies in the freshwater European eel (Anguilla anguilla) suggest that the efficiency of OXPHOS is increased after acclimation to high hydrostatic pressure, decreasing the enzymatic activity of Complex II in red muscle, whereas that of Complex IV is significantly increased . This situation would enable a reduction in the electron leak and the optimisation of the respiratory chain. Similarly, more recent studies in gilthead sea bream have revealed that the gene expression ratio of the enzyme subunits of Complexes I and IV is altered in heart and liver tissue during the recovery state after severe hypoxia exposure . Thus, as reviewed by , it is now evident that variations in the mitochondrial efficiency of ATP production exist among individuals, populations and environments, and even within the same individual over time. This spatial and temporal variability in mitochondrial machinery adds an additional layer of complexity to the regulation of energy metabolism, and the maintenance of aerobic metabolism is becoming recognised as a primary hypoxia survival strategy in most organisms, including fish . Even so, the usage of transcriptomic analysis with other experimental approaches related to mitochondrial activity and respiration would be necessary for the better understanding about the proposed re-adjustment of mitochondrial function in hypoxia-challenged fish.
Conclusions and future perspectives
The authors are grateful to M.A. González for her excellent technical assistance with sampling and PCR analyses.
This work was financially supported by a grant from the European Commission of the European Union under the Horizon 2020 research infrastructure project AQUAEXCEL2020 (652831). Additional funding was provided by Generalitat Valenciana (PROMETEOII/2014/085). JAMS received a Postdoctoral Research Fellowship (Juan de la Cierva-Formación, Reference FJCI-2014-20,161) from MINECO.
Availability of data and materials
The datasets supporting the conclusions of this study are included within the article and its additional Supporting Information file.
JACG and JPS conceived and designed the study. ABN, JACG and JAMS performed the experimental procedures. JAMS, JACG and JPS analysed and interpreted the data. JAMS and JPS drafted the original manuscript. All authors reviewed, edited and approved the final manuscript.
All procedures were approved by the Ethics and Animal Welfare Committee of Institute of Aquaculture Torre de la Sal and carried out according to the National (Royal Decree RD53/2013) and the current EU legislation (2010/63/EU) on the handling of experimental fish.
Consent for publication
The authors declare that they have no competing interests.
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- Fry FEJ. Effects of the environment on animal activity. Univ Tor Stud Biol Ser. 1947;68:5–62.Google Scholar
- Fry FEJ. The effect of environmental factors on the physiology of fish. In: Hroar WS, Randall DJ, editors. Fish physiology. Environmental relations and behavior 6. New York: Academic Press, Elsevier; 1971. p. 1–87.Google Scholar
- Pörtner HO, Grieshaber MK. Critical PO2 (s) in oxyconforming and oxyregulating animals gas exchange, metabolic rate and the mode of energy production. In: Bicudo JEPW, editor. The vertebrate gas transport cascade adaptations to environment and mode of life. Boca Raton: CRC Press; 1993. p. 330–57.Google Scholar
- Saravanan S, Geurden I, Figueiredo-Silva AC, Kaushik SJ, Haidar MN, Verreth JA, Schrama JW. Control of voluntary feed intake in fish: a role for dietary oxygen demand in Nile tilapia (Oreochromis niloticus) fed diets with different macronutrient profiles. Br J Nutr. 2012;108:1519–29.View ArticlePubMedGoogle Scholar
- Remen M, Nederlof MAJ, Folkedal O, Thorsheim G, Sitjà-Bobadilla A, Pérez-Sánchez J, Oppedal F, Olsen RE. Effect of temperature on the metabolism, behavior and oxygen requirements of Sparus aurata. Aquacult Env Interac. 2015;7:115–23.View ArticleGoogle Scholar
- Remen M, Sievers M, Torgersen T, Oppedal F. The oxygen threshold for maximal feed intake of Atlantic salmon post-smolts is highly temperature-dependent. Aquaculture. 2016;464:582–92.View ArticleGoogle Scholar
- Lushchak VI, Bagnyukova TV. Effects of different environmental oxygen levels on free radical processes in fish. Comp Biochem Physiol B-Biochem Mol Biol. 2006;144:283–9.View ArticlePubMedGoogle Scholar
- Bermejo-Nogales A, Calduch-Giner JA, Pérez-Sánchez J. Tissue-specific gene expression and functional regulation of uncoupling protein 2 (UCP2) by hypoxia and nutrient availability in gilthead sea bream (Sparus aurata): implications on the physiological significance of UCP1-3 variants. Fish Physiol Biochem. 2014;40:751–62.View ArticlePubMedGoogle Scholar
- Khacho M, Tarabay M, Patten D, Khacho P, MacLaurin JG, Guadagno J, Bergeron R, Cregan SP, Harper M-E, Park DS, Slack RS. Acidosis overrides oxygen deprivation to maintain mitochondrial function and cell survival. Nat Commun. 2014;5:3550.View ArticlePubMedPubMed CentralGoogle Scholar
- Hoppeler H, Vogt M. Muscle tissue adaptations to hypoxia. J Exp Biol. 2001;204:3133–9.PubMedGoogle Scholar
- Murray AJ. Metabolic adaptation of skeletal muscle to high altitude hypoxia: how new technologies could resolve the controversies. Genome Med. 2009;1:117.View ArticlePubMedPubMed CentralGoogle Scholar
- Gamboa JL, Andrade FH. Muscle endurance and mitochondrial function after chronic normobaric hypoxia: contrast of respiratory and limb muscles. Pflug Arch Eur J Physiol. 2012;463:327–38.View ArticleGoogle Scholar
- Donohoe PH, West TG, Boutilier RG. Respiratory, metabolic and acid–base correlates of aerobic metabolic rate reduction in overwintering frogs. Am J Physiol-Reg I. 1998;43:R704–10.Google Scholar
- Gracey AY, Troll JV, Somero GN. Hypoxia-induced gene expression profiling in the euryoxic fish Gillichthys mirabilis. Proc Natl Acad Sci U S A. 2001;98:1993–8.View ArticlePubMedPubMed CentralGoogle Scholar
- Everett MV, Antal CE, Crawford DL. The effect of short-term hypoxic exposure on metabolic gene expression. J Exp Zool Part A. 2012;317:9–23.View ArticleGoogle Scholar
- Vanderplancke G, Claireaux G, Quazuguel P, Madec L, Ferraresso S, Sévère A, Zambonino-Infante JL, Mazurais D. Hypoxic episode during the larval period has long-term effects on European sea bass juveniles (Dicentrarchus labrax). Mar Biol. 2015;162:367–76.View ArticleGoogle Scholar
- Stier A, Bize P, Schull Q, Zoll J, Singh F, Geny B, Gros F, Royer C, Massemin S, Criscuolo F. Avian erythrocytes have functional mitochondria, opening novel perspectives for birds as animal models in the study of ageing. Front Zool. 2013;10:33.View ArticlePubMedPubMed CentralGoogle Scholar
- Calduch-Giner JA, Echasseriau Y, Crespo D, Baron D, Planas JV, Prunet P, Pérez-Sánchez J. Transcriptional assessment by microarray analysis and large-scale meta-analysis of the metabolic capacity of cardiac and skeletal muscle tissues to cope with reduced nutrient availability in gilthead sea bream (Sparus aurata L.). Mar Biotechnol. 2014;16:423–35.View ArticlePubMedGoogle Scholar
- Bermejo-Nogales A, Calduch-Giner JA, Pérez-Sánchez J. Unraveling the molecular signatures of oxidative phosphorylation to cope with the nutritially changing metabolic capabilities of liver and muscle tissues in farmed fish. PLoS One. 2015;10(4):e0122889.View ArticlePubMedPubMed CentralGoogle Scholar
- Bermejo-Nogales A, Nederlof M, Benedito-Palos L, Ballester-Lozano GF, Folkedal O, Olsen RE, Sitjà-Bobadilla A, Pérez-Sánchez J. Metabolic and transcriptional responses of gilthead sea bream (Sparus aurata) to environmental stress: new insights in fish mitochondrial phenotyping. Gen Comp Endocrinol. 2014a;205:305–15.View ArticlePubMedGoogle Scholar
- Shimizu M, Swanson P, Fukada H, Hara A, Dickhoff WW. Comparison of extraction methods and assay validation for salmon insulin-like growth factor-I using commercially available components. Gen Comp Endocrinol. 2000;119:26–36.View ArticlePubMedGoogle Scholar
- Vega-Rubín de Celis S, Gómez-Requeni P, Pérez-Sánchez J. Production and characterization of recombinantly derived peptides and antibodies for accurate determinations of somatolactin, growth hormone and insulin-like growth factor-I in European sea bass (Dicentrarchus labrax). Gen Comp Endocrinol. 2004;139:266–77.View ArticlePubMedGoogle Scholar
- Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCt method. Methods. 2001;25:402–8.View ArticlePubMedGoogle Scholar
- Kadenbach B, Ramzan R, Wen L, Vogt S. New extension of the Mitchell theory for oxidative phosphorylation in mitochondria of living organism. BBA-Gen Subj. 1800;2010:205–12.Google Scholar
- Hochachka PW, Buck LT, Doll CJ, Land SC. Unifying theory of hypoxia tolerance: molecular/metabolic defense and rescue mechanisms for surviving oxygen lack. Proc Natl Acad Sci U S A. 1996;93:9493–8.View ArticlePubMedPubMed CentralGoogle Scholar
- Pérez-Jiménez A, Peres H, Rubio VC, Oliva-Teles A. The effect of hypoxia on intermediary metabolism and oxidative status in gilthead sea bream (Sparus aurata) fed on diets supplemented with methionine and white tea. Comp Biochem Physiol C-Toxicol Pharmacol. 2012;155:506–16.View ArticlePubMedGoogle Scholar
- Wood SC, Johansen K. Adaptation to hypoxia by increased HbO2 affinity and decreased red cell ATP concentration. Nat New Biol. 1972;237:278–9.View ArticlePubMedGoogle Scholar
- Soivio A, Nikinmaa M, Westman K. The blood oxygen binding properties of hypoxic Salmo gairdneri. J Comp Physiol. 1980;136(1):83–7.View ArticleGoogle Scholar
- Storey KB. Regulation of hypometabolism: insights into epigenetic controls. J Exp Biol. 2015;218(1):150–9.View ArticlePubMedGoogle Scholar
- Dalla Via J, Van den Thillart G, Cattani O, Cortesi P. Behavioural responses and biochemical correlates in Solea solea to gradual hypoxic exposure. Can J Zool. 1998;76:2108–13.View ArticleGoogle Scholar
- Virani NA, Rees BB. Oxygen consumption, blood lactate and inter-individual variation in the gulf killifish, Fundulus grandis, during hypoxia and recovery. Comp Biochem Physiol A-Mol Integr Physiol. 2000;126:397–405.View ArticlePubMedGoogle Scholar
- Mommsen TP, Vijayan MM, Moon TW. Cortisol in teleosts: dynamics, mechanisms of action, and metabolic regulation. Rev Fish Biol Fish. 1999;9:211–68.View ArticleGoogle Scholar
- Dyer AR, Upton Z, Stone D, Thomas PM, Soole KL, Higgs N, Quinn K, Carragher JF. Development and validation of a radioimmunoassay for fish insulin-like growth factor I (IGF-I) and the effect of aquaculture related stressors on circulating IGF-I levels. Gen Comp Endocrinol. 2004;135:268–75.View ArticlePubMedGoogle Scholar
- Saera-Vila A, Calduch-Giner JA, Prunet P, Pérez-Sanchez J. Dynamics of liver GH/IGF axis and selected stress markers in juvenile gilthead sea bream (Sparus aurata) exposed to acute confinement: differential stress response of growth hormone receptors. Comp Biochem Physiol A-Mol Integr Physiol. 2009;154:197–203.View ArticlePubMedGoogle Scholar
- Brown-Borg HM, Rakoczy SG, Romanick MA, Kennedy MA. Effects of growth hormone and insulin like growth factor-1 on hepatocyte antioxidative enzymes. Exp Biol Med. 2002;227:94–104.Google Scholar
- Brown-Borg HM, Rakoczy SG. Growth hormone administration to long-living dwarf mice alters multiple components of the antioxidative defense system. Mech Aging Dev. 2003;124:1013–24.View ArticlePubMedGoogle Scholar
- Ton C, Stamatiou D, Liew CC. Gene expression profile of zebrafish exposed to hypoxia during development. Physiol Genomics. 2003;13:97–106.View ArticlePubMedGoogle Scholar
- Woods IG, Imam FB. Transcriptome analysis of severe hypoxic stress during development in zebrafish. Genomics data. 2015;6:83–8.View ArticlePubMedPubMed CentralGoogle Scholar
- Robertson CE, Wright PA, Köblitz L, Bernier NJ. Hypoxia-inducible factor-1 mediates adaptive developmental plasticity of hypoxia tolerance in zebrafish, Danio rerio. P Royal Soc Lond B Bio. 2014;281(1786):20140637.View ArticleGoogle Scholar
- Köblitz L, Fiechtner B, Baus K, Lussnig R, Pelster B. Developmental expression and hypoxic induction of hypoxia inducible transcription factors in the zebrafish. PLoS One. 2015;10(6):e0128938.View ArticlePubMedPubMed CentralGoogle Scholar
- van der Meer DL, van den Thillart GE, Witte F, de Bakker MA, Besser J, Richardson MK, Spaink HP, Leito JTD, Bagowski CP. Gene expression profiling of the long-term adaptive response to hypoxia in the gills of adult zebrafish. Am J Physiol-Reg I. 2005;289:1512–9.Google Scholar
- Pfanner N, Meijer M. Mitochondrial biogenesis: the tom and Tim machine. Curr Biol. 2010;7:100–3.View ArticleGoogle Scholar
- Smits P, Smeitink J, van den Heuvel L. Mitochondrial translation and beyond: processes implicated in combined oxidative phosphorylation deficiencies. J Biomed Biotechnol. 2010;2010:737385.View ArticlePubMedPubMed CentralGoogle Scholar
- Ljubicic V, Joseph AM, Saleem A, Uguccioni G, Collu-Marchese M, Lai RY, Nguyen LM-D, Hood D. A. Transcriptional and post-transcriptional regulation of mitochondrial biogenesis in skeletal muscle: effects of exercise and aging. BBA-Gen Subj. 2010;1800:223–34.View ArticleGoogle Scholar
- Faou P, Hoogenraad NJ. Tom34: a cytosolic cochaperone of the Hsp90/Hsp70 protein complex involved in mitochondrial protein import. BBA-Mol Cell Res. 2012;1823:348–57.Google Scholar
- Currie S, Tufts BL, Moyes CD. Influence of bioenergetic stress on heat shock protein gene expression in nucleated red blood cells of fish. Am J of Physiol-Reg I. 1999;276:990–6.Google Scholar
- Ferree A, Shirihai O. Mitochondrial dynamics: the intersection of form and function. In: Kadenbach B, editor. Mitochondrial oxidative Phosphorylation. New York: Springer; 2012. p. 13–40.View ArticleGoogle Scholar
- Anesti V, Scorrano L. The relationship between mitochondrial shape and function and the cytoskeleton. BBA–Bioenerg. 2006;1757:692–9.Google Scholar
- Liesa M, Borda-d’Água B, Medina-Gómez G, Lelliott CJ, Paz JC, Rojo M, Palacín M, Vidal-Puig A, Zorzano A. Mitochondrial fusion is increased by the nuclear coactivator PGC-1β. PLoS One. 2008;3(10):e3613.View ArticlePubMedPubMed CentralGoogle Scholar
- Patti ME, Butte AJ, Crunkhorn S, Cusi K, Berria R, Kashyap S, Miyazaki Y, Kohane I, Costello M, Saccone R, Landaker EJ, Goldfine AB, Mun E, DeFronzo R, Finlayson J, Kahn CR, Mandarino LJ. Coordinated reduction of genes of oxidative metabolism in humans with insulin resistance and diabetes: potential role of PGC1 and NRF1. P Nat Acad Sci USA. 2003;100:8466–71.View ArticleGoogle Scholar
- Arany Z, He H, Lin J, Hoyer K, Handschin C, Toka O, Ahmad F, Matsui T, Chin S, Wu P-H, Rybkin II, Shelton JM, Manieri M, Cinti S, Schoen FJ, Bassel-Duby R, Rosenzweig A, Ingwall JS, Spiegelman BM. Transcriptional coactivator PGC-1α controls the energy state and contractile function of cardiac muscle. Cell Metab 2005;1:259-271.Google Scholar
- Leone TC, Lehman JJ, Finck BN, Schaeffer PJ, Wende AR, Boudina S, Courtois M, Wozniak DF, Sambandam N, Bernal-Mizrachi C, Chen Z, Holloszy JO, Medeiros DM, Schmidt RE, Saffitz JE, Abel ED, Semenkovich CF, Kelly DP. PGC-1α deficiency causes multi-system energy metabolic derangements: muscle dysfunction, abnormal weight control and hepatic steatosis. PLoS Biol. 2005;3(4):e101.View ArticlePubMedPubMed CentralGoogle Scholar
- Lelliott C, Medina-Gomez G, Petrovic N, Kis A, Feldmann HM, Bjursell M, Parker N, Curtis K, Campbell M, Hu P, Zhang D, Litwin SE, Zaha VG, Fountain KT, Boudina S, Jimenez-Linan M, Blount M, Lopez M, Meirhaeghe A, Bohlooly-Y M, Storlien L, Strömstedt M, Snaith M, Oresic M, Abel ED, Cannon B, Vidal-Puig A. Ablation of PGC-1b results in defective mitochondrial activity, thermogenesis, hepatic function, and cardiac performance. PLoS Biol. 2006;4(11):e369.View ArticlePubMedPubMed CentralGoogle Scholar
- Holloszy JO. Biochemical adaptations in muscle effects of exercise on mitochondrial oxygen uptake and respiratory enzyme activity in skeletal muscle. J Biol Chem. 1967;242:2278–82.PubMedGoogle Scholar
- Zerbetto E, Vergani L, Dabbeni-Sala F. Quantification of muscle mitochondrial oxidative phosphorylation enzymes via histochemical staining of blue native polyacrylamide gels. Electrophoresis. 1997;18:2059–64.View ArticlePubMedGoogle Scholar
- Guderley H. Metabolic responses to low temperature in fish muscle. Biol Rev. 2004;79:409–27.View ArticlePubMedGoogle Scholar
- Zhu Z, Yao J, Johns T, Fu K, De Bie I, Macmillan C, Cuthbert AP, Newbold RF, Wang J, Chevrette M, Brown GK, Brown RM, Shoubridge EA. SURF1, encoding a factor involved in the biogenesis of cytochrome c oxidase, is mutated in Leigh syndrome. Nat Genet. 1998;20:337–43.View ArticlePubMedGoogle Scholar
- Antonicka H, Mattman A, Carlson CG, Glerum DM, Hoffbuhr KC, Leary SC, Kennaway NG, Shoubridge EA. Mutations in COX15 produce a defect in the mitochondrial heme biosynthetic pathway, causing early-onset fatal hypertrophic cardiomyopathy. Am J Hum Genet. 2003;72:101–14.View ArticlePubMedGoogle Scholar
- Williams SL, Valnot I, Rustin P, Taanman JW. Cytochrome c oxidase subassemblies in fibroblast cultures from patients carrying mutations in COX10, SCO1, or SURF1. J Biol Chem. 2004;279:7462–9.View ArticlePubMedGoogle Scholar
- Stiburek L, Vesela K, Hansikova H, Pecina P, Tesarova M, Cerna L, Houstek J, Zeman J. Tissue-specific cytochrome c oxidase assembly defects due to mutations in SCO2 and SURF1. Biochem J. 2005;392:625–32.View ArticlePubMedPubMed CentralGoogle Scholar
- Smith D, Gray J, Mitchell L, Antholine WE, Hosler JP. Assembly of cytochrome-c oxidase in the absence of assembly protein Surf1p leads to loss of the active site heme. J Biol Chem. 2005;280:17652–6.View ArticlePubMedGoogle Scholar
- García-Horsman JA, Barquera B, Rumbley J, Ma J, Gennis RB. The superfamily of heme-copper respiratory oxidases. J Bacteriol. 1994;176(18):5587.View ArticlePubMedPubMed CentralGoogle Scholar
- Lenka N, Vijayasarathy C, Mullick J, Avadhani NG. Structural organization and transcription regulation of nuclear genes encoding the mammalian cytochrome c oxidase complex. Prog Nucl Res Molec Biol. 1998;61:309–44.View ArticleGoogle Scholar
- Ghezzi D, Zeviani M. Assembly factors of human mitochondrial respiratory chain complexes: physiology and Pathophysiology. In: Kadenbach B, editor. Mitochondrial oxidative Phosphorylation. New York: Springer; 2012. p. 65–106.View ArticleGoogle Scholar
- St-Pierre J, Brand MD, Boutilier RG. The effect of metabolic depression on proton leak rate in mitochondria from hibernating frogs. J Exp Biol. 2000;203:1469–76.PubMedGoogle Scholar
- Theron M, Guerrero F, Sebert P. Improvement in the efficiency of oxidative phosphorylation in the freshwater eel acclimated to 10.1 MPa hydrostatic pressure. J Exp Biol. 2000;203:3019–23.PubMedGoogle Scholar
- Magnoni L, Martos-Sitcha JA, Queiroz A, Calduch-Giner JA, Magalhães Gonçalves JF, Rocha CMR, Abreu HT, Schrama JW, Ozorio ROA, Pérez-Sánchez J. Dietary supplementation of heat-treated Gracilaria and Ulva seaweeds enhanced acute hypoxia tolerance in gilthead Seabream (Sparus aurata). Biol Open. 2017;6(6):897–908. doi:https://doi.org/10.1242/bio.024299.View ArticlePubMedPubMed CentralGoogle Scholar
- Salin K, Auer SK, Rey B, Selman C, Metcalfe NB. Variation in the link between oxygen consumption and ATP production, and its relevance for animal performance. Proc R Soc B. 2015;282:20151028.View ArticlePubMedPubMed CentralGoogle Scholar
- Rogers NJ, Urbina MA, Reardon EE, McKenzie DJ, Wilson RW. A new analysis of hypoxia tolerance in fishes using a database of critical oxygen level (Pcrit). Conserv Physiol. 2016;4(1):cow012.View ArticlePubMedPubMed CentralGoogle Scholar