Fig. 7

Formation of the suckers. a-b epifluorescent images of suckers on arm II (a) and arm IV (b) at stage 30 stained for DAPI to visualize cell nuclei in cyan. c-h DIC images of suckers on arm II (c-e) and arm IV (f-h) at stages indicated in the bottom left corner. Arms are oriented with distal to the left, aboral to the top. i-n merged confocal image stacks of individual suckers on arm II (i-k) and arm IV (l-n) labeled with phallacidin to visualize F-actin in green and for acetylated tubulin to visualize the nerve tracts in red at stages indicated in the bottom left corner. Inset in (k) shows an epifluorescent image of a sucker of at stage 30 stained for phallacidin. Arrowheads point at area of sucker formation. ac, acetabulum; an, acetabular nerve; c, circular muscle; cn, connective nerve; e, extrinsic muscle; ec, extrinsic circular muscle; cy, cylinder, inf, infundibulum; me, meridional muscle; s, stalk; sph, sphincter muscle. Scale bars: 100 μm in a, 50 μm in c, and 10 μm in i, l