- Short report
- Open Access
Self-sacrifice in 'desperado' contests between relatives
© Cronin and Monnin; licensee BioMed Central Ltd. 2010
- Received: 15 April 2010
- Accepted: 13 October 2010
- Published: 13 October 2010
Intra-specific competition occurs in all animal species and can lead to escalated conflict. Overt fighting entails the risk of injury or death, and is usually avoided through the use of conventions or pre-fight assessments. However, overt fighting can be expected when value of the contest outweighs the value of the future, as contestants have little or nothing to lose. In these situations, respect for conventions and asymmetries between contestants can break down, and overt fighting becomes more likely (the desperado effect). Such conditions can arise in contests between queens over colony ownership in social insects, because the value of inheriting a colony of potentially thousands of helpers is huge and queens may have very limited alternative reproductive options. However, in social species the balance of possible outcomes may be influenced by inclusive fitness, as contestants are often relatives. Here we present a simple model based on social insects, which demonstrates that not fighting can be selectively advantageous when there is a risk posed by fighting to inclusive fitness, even when not fighting is likely to result in death. If contestants are related, a loser can still gain indirect fitness through the winner, whereas fighting introduces a risk that both queens will die and thereby obtain zero inclusive fitness. When relatedness is high and fighting poses a risk of all contestants dying, it can be advantageous to cede the contest and be killed, rather than risk everything by fighting.
- Social Insect
- Inclusive Fitness
- Direct Fitness
- Fighting Ability
- Indirect Fitness
Intra-specific contests, primarily over resources and reproductive opportunities, are a ubiquitous aspect of animal behaviour. Such contests are highly significant as they can determine a large proportion (or indeed all) of an animal's lifetime fitness, and pose the risk of injury or death when they escalate to fights. Because of these risks, contest resolution is often achieved without fighting, through processes of mutual and/or self-assessment [1–3]. However, models of animal contest predict that escalated contests leading to fatal fighting become more likely when the outcome of a contest represents a greater proportion of lifetime fitness [1, 4]. That is, when contestants have little chance of fitness gains outside of the contest in question, fights should occur regardless of the risks involved because there is much to gain and little or nothing to lose [1, 5]. Furthermore, kinship between contestants will not prevent escalated contests as long as the ratio between the value of the future and the value of the contest is small [4, 5]. Fatal fighting can arise when Vo/V < 1 - r, where Vo and V are respectively the value of the future and of the current contest in terms of lifetime fitness, and r is the coefficient of relatedness between contestants . Grafen  termed this the 'desperado effect', to describe the point where respect for conventions and asymmetries between contestants breaks down.
Escalated fighting occurs between relatives in a 'desperado' context in several circumstances: among male fig wasps and ants over mating opportunities [6–9], among nursery siblings over access to food resources in birds and mammals when young are overproduced [10, 11], and among social insect queens over resource inheritance [3, 12]. Contests are of utmost significance among queens of social insects as, in many species, colonies of potentially thousands of individuals are headed by one reproductive queen. Becoming queen thus represents a potentially huge fitness payoff, because once victorious, a queen is insulated from further environmental risks by the workers and can live for many years. Contests over the queen role can occur in normally monogynous species when several queens temporarily co-occur in one nest, such as during queen replacement, during colony reproduction via fission, and in pleometrotic foundress associations. In each case monogyny is soon restored: excess queens are culled by workers or killed during direct fighting between queens . In such situations, there are usually very limited or no other reproductive opportunities for candidate queens. Thus, we would expect strong conflict between queens over colony inheritance to give rise to escalated fighting, and indeed this occurs in a range of species with variable degrees of relatedness between contestants (eg: honeybees [13, 14], bumblebees , ants [16–18] and wasps ).
In the ant Aphaenogaster senilis, contests over colony inheritance occur during emergency queen replacement following orphaning. A few full-sister replacement queens are produced and the first-born queen is usually behaviourally dominant over later emerging queens . Late-born queens apparently cede to the first-born queen without fighting and are eventually killed by workers. In experimental groups with two queens, second-born queens did not fight and usually died, even when the first-born had one mandible ablated and could not bite effectively . Alternative reproductive opportunities for late-born queens are almost non-existent, and thus ceding to the first-born is effectively a form of suicide . This lack of reciprocal fighting apparently contradicts the predictions of contest models.
However, models based on hawk-dove games generally assume that in an encounter between two hawks, one will survive while the other is killed. This does not take into account the possibility that both contestants die and thus obtain zero inclusive fitness. In do-or-die contests such as those over colony inheritance, it is possible that the victor sustains a mortal injury during the fight, and subsequently dies or is killed by workers, leading to the death of both contestants [eg: ]. Furthermore, as replacement queens are reared from existing queen-laid eggs in most social hymenoptera (workers typically cannot produce female brood), further queens cannot be reared once existing brood are exhausted, and there is a risk of colony death through failure to requeen.
A queen that cedes and is killed can still gain indirect fitness though the victorious queen if they are related, while at the same time avoiding the risk of a zero fitness outcome. Thus, the risk of both contestants dying may impose a significant cost to fighting if relatedness between the contestants is high . Highly related contestants in 'desperado' contests, such as late-born A. senilis queens, may secure more indirect fitness by not fighting than direct fitness from victory when the latter is devalued by the risk of both queens dying . Here, we formalise this reasoning by developing a simple model to explain how not fighting can be selectively advantageous in a 'desperado' contest between relatives. We focus on 'desperado' situations, where contestant's alternative options for fitness gain are negligible or absent. The area of contests outside of this has been explored by a suite of reproductive skew models (reviewed in ). Our model was developed to explain behaviour observed in social insect colonies, and we initially focus on these systems. The applicability of the rationale to other taxa is explored in the discussion.
Life history of A. senilis
A. senilis is a monogynous, monandrous ant common to the Mediterranean basin, that reproduces by colony fission: queens are born with short wings and cannot fly, and only disperse with groups of workers on foot [23, 24]. Colonies consist of around 1,300 workers and are monodomous. Colonies temporarily contain multiple queens when new queens are reared during preparation for colony fission or when the queen has died and must be replaced. Because of haplodiploidy, these new queens are full-sisters and are related by 0.75. The frequency of requeening in the field is unknown, but orphaning in the lab results in the production of one to six replacement queens: in one experiment 30 orphaned colonies produced 2.0 ± 1.1 queens , and in a second experiment 60 orphaned groups produced 2.8 ± 1.3 queens each . New queens are produced 14-17 days apart on average. Only one queen survives, and it is usually the first-born who inherits the colony while subsequently born queens are killed [20, 21].
Many strictly monogynous social insects go through transient polygynous phases: pleometrosis (founding of nests by multiple queens) occurs in many species as a regular mode of colony initiation , and fission occurs in a wide range of species, some of which are monogynous (eg: honeybees and army ants ). The frequency of queen replacement in nature is largely unknown, but it may be common in species that reproduce via fission (Cronin et al. unpublished data). Obligate monogyny in social insects is widespread, despite the fact that polygyny can be favoured for a variety of reasons including increased colony productivity, increased colony longevity, and high mortality of dispersing queens [, pp 126-134].
Abbreviations and variables used in the model.
The contestant holding the advantage in some form of asymmetry, which will thus win the contest if no overt fighting occurs.
The opponent, who will lose the contest unless it fights Q1.
Fitness value of the contest (ie: lifetime fitness of the queen that inherits the colony).
Fitness value of the future (ie: outside of the contest). Vo is here set to 0, as queens have no reproductive opportunities outside the contest.
0 to 1
The probability of Q1 winning an overt fight, here expressed as a linear function of relative fighting ability: a = ½(f+1)
0 to 1
The probability that fighting will result in the death of both queens, based on the mortality index and relative fighting ability, such that d =m for contestants of equal fighting ability, and declines with increasing disparity in fighting ability following d = m. e-αf²
0 to 1
Genetic relatedness between contestants.
-1 to 1
Relative fighting ability of Q1. When f = -1, Q1 has a lower fighting ability than Q2; when f = 0, Q1 and Q2 have equal fighting abilities; and when f = +1, Q1 has a higher fighting ability.
0 to 1
Mortality index. A factor based on the lethality of unrestrained combat for the species in question, expressed as a probability that a fight between contestants of equal fighting ability will lead to the death of both contestants.
A factor defining the shape of the curve describing the effect of relative fighting ability on the chance of mortality of both queens (Fig. 3).
For simplicity, we consider the contest in isolation, without regard to strategies employed in the rest of the population. We assume that the winning queen gains equal fitness (V) whether a fight occurs or not, as the duration of conflict (a few days) is negligible compared to the length of time fitness is gained (a few years), and non lethal injuries sustained during a fight have no effect on reproductive value since the queens accrue resources indirectly through workers. The effect of relaxing these assumptions is discussed later. We further assume that workers select queens based on some criteria of asymmetry between queens, as is common in species where intra-specific contests arise (eg: order of arrival or age [20, 28, 29]). This asymmetry gives one competitor some advantage, and the queen which holds the advantage in the asymmetry (Q1) will win the contest if no fighting occurs (workers will kill the other queen). The advantage may be uncorrelated with the individual's intrinsic quality , and we consider it independently of fighting ability and reproductive value. In A. senilis, the asymmetry is determined by birth order, and the first born queen (Q1) generally inherits. How workers select one queen over the other is generally unclear, but may be related to a need to expedite the queen replacement process [20, 21], or because of queen manipulation [31, 32].
The possible contest outcomes are thus a) Q1 inherits the colony, either through killing Q2, or when Q2 is killed by workers in the case that no queen-queen fight occurs, b) Q2 inherits the colony by killing Q1, or c) both queens are killed fighting. Queens can gain direct fitness through inheriting the colony or indirect fitness when a relative inherits the colony. The fitness payoff for a given queen depends on the probability of winning the fight if they do fight, the risk of both queens dying, and the genetic relatedness between the queens.
Where a is the probability of Q1 winning an overt fight and 1 - a is the probability of Q2 winning. Genetic relatedness between contestants is denoted by r, and d represents the probability of a zero fitness outcome because both queens are killed in the contest.
Overt fighting can be expected in animal contests when the value of the contest is high relative to the value of the future  as contestants have nothing to lose. However, in contests between relatives there is something to lose, because fighting poses the risk of both contestants dying: in fighting with a related opponent, a losing individual can risk inclusive fitness loss by fatally injuring its competitor. Our results suggest that relatedness between contestants can lead to restraint under specific circumstances, even when not fighting means death and there are no alternative future reproductive opportunities. Our model indicates that this is possible when three conditions are met: i) contests are 'desperado' contests, with one winner and no alternative opportunities for fitness gains for the loser, ii) fighting poses a risk of mortality to the victor and, iii) contestants are highly related.
We have assumed that Q2 decides for herself how to act in a given situation. An alternative possibility is that her behaviour is manipulated by Q1 and/or workers, both of which benefit from Q1 inheriting if she is fit. It is unclear whether Q1 has the means of manipulating Q2 (eg: through chemical signals), but workers presumably do, as they control the amount of food Q2 receives as a larva, which may allow them to control her growth rate and hence delay her emergence, thereby advantaging Q1. Mechanisms of influencing offspring development and contest outcomes have also been described in vertebrates . However, whether Q2 is manipulated or not in her decision to fight in A. senilis awaits investigation.
We employ the simplifying assumption of considering our contest in isolation, without regard to population-wide strategies. In reality, model parameters such as the value of the contest V are not independent of strategies employed by other colonies in the population, and this should be considered in a more comprehensive model. Furthermore, we assume a 2-queen contest arena and, while our model is applicable to most A. senilis colonies where only a few queens are produced, in some colonies Q1 may need to win multiple consecutive contests, and this may be the norm in other species. In such cases, an injury sustained in a fight will lower the relative fighting ability of the queen for the next fight. For instance, if Q1 wins the fight but is injured in the process she may have a lower fighting ability against a third queen. Nonetheless, unless Q3 is a markedly better fighter, she will still elect not to fight in order not to jeopardize her inclusive fitness (Fig. 3 with -0.25 < f <0). Conversely, if Q3 is a much stronger fighter (f < - 0.4) there is little risk that the queens will kill one another and Q3 will fight. However, in A. senilis late born queens do not fight even when Q1 has had one mandible ablated and cannot bite effectively (ie: f is close to -1) . It may be that in this species, queens have not been selected to assess fighting abilities because queens are full-sisters and differ too little in fighting ability to ever make fighting a successful strategy for Q2 (Fig. 1).
Clearly, the conditions required for self sacrifice to occur are restrictive, and probably do not arise in most cases of animal contests because i) relatedness between contestants is insufficiently high or ii) there are other opportunities for fitness acquisition outside the focal contest. This includes contests among ant queens following pleometrosis: as foundresses are usually unrelated  overt fighting eventuates (eg: Lasius niger [16, 18], Messor pergandei , Azteca xanthacroa , Solenopsis wagneri (monogyne form) [12, 17]).
Examples of contests between relatives in a potential 'desperado' context in social insects.
Reason for contest
Relatedness to competitors
Overt reciprocal fighting?
Colony inheritance during requeening
Colony inheritance during requeening or fission
Low (polyandry) to high (parthenogenesis)
, Chéron unpublished data
Colony inheritance during requeening or fission
Normal colony cycle
Moderate (mother - daughter) to High (full-sisters)
N (workers kill new queens quickly)
Colony inheritance during colony cycle
Y (mother and daughter)
Males also fight over females in many fig wasps [8, 9, 38], where the intensity of fights varies between species from causing no injury  to causing a high level of life-threatening injuries [38, 39]. Here, self-sacrifice is not expected because competing males are not highly related in many species  and males may avoid fights they are unlikely to win .
In vertebrates, siblings fight over food in some birds and mammals producing more young than can be reared . However, in many cases these may not be true desperado contests as all young may be reared in exceptionally bountiful years, even in 'obligately' siblicidal species [11, 41]. Nonetheless, examples of overt fighting leading to siblicide exist for a range of avian species and some mammals [ and references therein], in which junior young are usually killed by senior siblings. Conditions favouring self-sacrifice may not arise because relatedness between vertebrate siblings (r = 0.5) is lower than for Hymenopteran full-sisters (r = 0.75) and, furthermore, development related asymmetries may be so extreme that the risk of contestants mortally injuring one another is negligible (ie: there is no risk to inclusive fitness), rendering self-sacrifice irrelevant. We predict that circumstances that can give rise to self-sacrifice are most likely to be found in monogynous and monandrous species of social insect that can requeen or that reproduce by colony fission, as relatedness is high and death of all but one contestant is inevitable. Stingless bees (Meliponini) fulfil these criteria, but queen-queen contests can be precluded by worker pre-emptively culling of queens  and details of queen-queen conflict are presently lacking. Closer investigation of contests in species where the specific conditions described arise could demonstrate further examples of self-sacrifice.
We thank Nicolas Loeuille, Mathieu Molet, Franjo Weissing and anonymous referees for comments which greatly improved the manuscript, and are grateful to Nicolas Loeuille for helpful discussion. This work was supported by the French Agence Nationale de la Recherche, grant ANR-06-BLAN-0268.
- Maynard-Smith JM, Price GR: Logic of Animal Conflict. Nature. 1973, 246: 15-18. 10.1038/246015a0.View ArticleGoogle Scholar
- Parker GA: Assessment strategy and evolution of fighting behavior. J Theor Biol. 1974, 47: 223-243. 10.1016/0022-5193(74)90111-8.View ArticlePubMedGoogle Scholar
- Dietemann V, Zheng HQ, Hepburn C, Hepburn HR, Jin SH, Crewe RM, Radloff SE, Hu FL, Pirk CWW: Self assessment in insects: honeybee queens know their own strength. PLoS ONE. 2008, 3: e1412-10.1371/journal.pone.0001412.PubMed CentralView ArticlePubMedGoogle Scholar
- Enquist M, Leimar O: The evolution of fatal fighting. Anim Behav. 1990, 39: 1-9. 10.1016/S0003-3472(05)80721-3.View ArticleGoogle Scholar
- Grafen A: The logic of divisively asymmetric contests - respect for ownership and the desperado effect. Anim Behav. 1987, 35: 462-467. 10.1016/S0003-3472(87)80271-3.View ArticleGoogle Scholar
- Kinomura K, Yamauchi K: Fighting and mating behaviors of dimorphic males in the ant Cardiocondyla wroughtoni. J Ethol. 1987, 5: 75-81. 10.1007/BF02347897.View ArticleGoogle Scholar
- Stuart RJ, Francoeur A, Loiselle R: Lethal fighting among dimorphic males of the ant, Cardiocondyla wroughtonii. Naturwissenschaften. 1987, 74: 548-549. 10.1007/BF00367076.View ArticleGoogle Scholar
- Hamilton WD: Wingless and fighting males in fig wasps and other insects. Sexual Selection and Reproductive Competition in Insects. Edited by: Blum MS, Blum NA. 1979, New York: Academic Press, 167-220.Google Scholar
- Greeff JM, van Noort S, Rasplus JY, Kjellberg F: Dispersal and fighting in male pollinating fig wasps. CR Biol. 2003, 326: 121-130. 10.1016/S1631-0691(03)00010-6.View ArticleGoogle Scholar
- Hofer H, East ML: Siblicide in Serengeti spotted hyenas: a long-term study of maternal input and cub survival. Behav Ecol Sociobiol. 2008, 62: 341-351. 10.1007/s00265-007-0421-3.View ArticleGoogle Scholar
- Drummond H, Rodriguez C, Vallarino A, Valderrabano C, Rogel G, Tobon E: Desperado siblings: uncontrollably aggressive junior chicks. Behav Ecol Sociobiol. 2003, 53: 287-296.Google Scholar
- Balas MT: Conditions favoring queen execution in young social insect colonies. Insect Soc. 2005, 52: 77-83. 10.1007/s00040-004-0774-9.View ArticleGoogle Scholar
- Gilley DC: The behavior of honey bees (Apis mellifera ligustica) during queen duels. Ethology. 2001, 107: 601-622. 10.1046/j.1439-0310.2001.00692.x.View ArticleGoogle Scholar
- Tarpy DR, Mayer MK: The effects of size and reproductive quality on the outcomes of duels between honey bee queens (Apis mellifera L.). Ethol Ecol Evol. 2009, 21: 147-153. 10.1080/08927014.2009.9522503.View ArticleGoogle Scholar
- Cameron SA, Jost MC: Mediators of dominance and reproductive success among queens in the cyclically polygynous Neotropical bumble bee Bombus atratus Franklin. Insect Soc. 1998, 45: 135-149. 10.1007/s000400050075.View ArticleGoogle Scholar
- Sommer K, Hölldobler B: Colony founding by queen association and determinants of reduction in queen number in the ant Lasius niger. Anim Behav. 1995, 50: 287-294. 10.1006/anbe.1995.0244.View ArticleGoogle Scholar
- Bernasconi G, Keller L: Reproductive conflicts in cooperative associations of fire ant queens (Solenopsis invicta). Proc R Soc Lond B. 1996, 263: 509-513. 10.1098/rspb.1996.0077.View ArticleGoogle Scholar
- Aron S, Steinhauer N, Fournier D: Influence of queen phenotype, investment and maternity apportionment on the outcome of fights in cooperative foundations of the ant Lasius niger. Anim Behav. 2009, 77: 1067-10.1016/j.anbehav.2009.01.009.View ArticleGoogle Scholar
- Nascimento FS, Tannure-Nascimento IC, Zucchi R: Behavioral mediators of cyclical oligogyny in the Amazonian swarm founding wasp Asteloeca ujhelyii (Vespidae, Polistinae, Epiponini). Insect Soc. 2004, 51: 17-23. 10.1007/s00040-003-0696-y.View ArticleGoogle Scholar
- Chéron B, Doums C, Fédérici P, Monnin T: Queen replacement in the monogynous ant Aphaenogaster senilis: supernumerary queens as life insurance. Anim Behav. 2009, 78: 1317-1325. 10.1016/j.anbehav.2009.08.016.View ArticleGoogle Scholar
- Cronin AL, Monnin T: Bourgeois queens and high stakes games in the ant Aphaenogaster senilis. Front Zool. 2009, 6: 24-10.1186/1742-9994-6-24.PubMed CentralView ArticlePubMedGoogle Scholar
- Buston PM, Zink AG: Reproductive skew and the evolution of conflict resolution: a synthesis of transactional and tug-of-war models. Behav Ecol. 2009, 20: 672-684. 10.1093/beheco/arp050.View ArticleGoogle Scholar
- Ledoux A: Un nouveau mode de bouturage de société chez la fourmi Aphaenogaster senilis Mayr. CR Acad Sci Ser D. 1971, 273: 83-85.Google Scholar
- Boulay R, Hefetz A, Cerdá X, Devers S, Francke W, Twele R, Lenoir A: Production of sexuals in a fission-performing ant: dual effects of queen pheromones and colony size. Behav Ecol Sociobiol. 2007, 61: 1531-1541. 10.1007/s00265-007-0385-3.View ArticleGoogle Scholar
- Bernasconi G, Strassmann JE: Cooperation among unrelated individuals: the ant foundress case. Trends Ecol Evol. 1999, 14: 477-482. 10.1016/S0169-5347(99)01722-X.View ArticlePubMedGoogle Scholar
- Peeters C, Ito F: Colony dispersal and the evolution of queen morphology in social Hymenoptera. Annu Rev Entomol. 2001, 48: 601-630. 10.1146/annurev.ento.46.1.601.View ArticleGoogle Scholar
- Crozier RH, Pamilo P: Evolution of Social Insect Colonies. 1996, Oxford: Oxford University PressGoogle Scholar
- Seppä P, Queller DC, Strassmann JE: Reproduction in foundress associations of the social wasp, Polistes carolina: conventions, competition, and skew. Behav Ecol. 2002, 13: 531-542. 10.1093/beheco/13.4.531.View ArticleGoogle Scholar
- Bridge C, Field J: Queuing for dominance: gerontocracy and queue-jumping in the hover wasp Liostenogaster flavolineata. Behav Ecol Sociobiol. 2007, 61: 1253-1259. 10.1007/s00265-007-0355-9.View ArticleGoogle Scholar
- Maynard Smith J: Evolution and the Theory of Games. 1982, Cambridge, UK: Cambridge University PressView ArticleGoogle Scholar
- Monnin T, Ratnieks FLW, Jones GR, Beard R: Pretender punishment induced by chemical signalling in a queenless ant. Nature. 2002, 419: 61-65. 10.1038/nature00932.View ArticlePubMedGoogle Scholar
- Tarpy DR, Fletcher DJC: "Spraying" behavior during queen competition in honey bees. J Insect Behav. 2003, 16: 425-437. 10.1023/A:1024884211098.View ArticleGoogle Scholar
- Drummond H, Rodriguez C, Schwabl H: Do mothers regulate facultative and obligate siblicide by differentially provisioning eggs with hormones?. Journal of Avian Biology. 2008, 39: 139-143. 10.1111/j.0908-8857.2008.04365.x.View ArticleGoogle Scholar
- Rissing SW, Pollock GB: Social-interaction among pleometrotic queens of Veromessor pergandei (Hymenoptera, Formicidae) during colony foundation. Anim Behav. 1986, 34: 226-233. 10.1016/0003-3472(86)90027-8.View ArticleGoogle Scholar
- Choe JC, Perlman DL: Social conflict and cooperation among founding queens in ants (Hymenoptera: Formicidae). The Evolution of Social Behavior in Insects and Arachnids. Edited by: Choe JC, Crespi BJ. 1997, Cambridge: Cambridge University Press, 392-406. full_text.View ArticleGoogle Scholar
- Heinze J, Hölldobler B, Yamauchi K: Male competition in Cardiocondyla ants. Behav Ecol Sociobiol. 1998, 42: 239-246. 10.1007/s002650050435.View ArticleGoogle Scholar
- Yamauchi K, Ishida Y, Hashim R, Heinze J: Queen-queen competition by precocious male production in multiqueen ant colonies. Curr Biol. 2006, 16: 2424-2427. 10.1016/j.cub.2006.10.007.View ArticlePubMedGoogle Scholar
- West SA, Murray MG, Machado CA, Griffin AS, Herre EA: Testing Hamilton's rule with competition between relatives. Nature. 2001, 409: 510-513. 10.1038/35054057.View ArticlePubMedGoogle Scholar
- Moore JC, Obbard DJ, Reuter C, West SA, Cook JM: Fighting strategies in two species of fig wasp. Anim Behav. 2008, 76: 315-322. 10.1016/j.anbehav.2008.01.018.View ArticleGoogle Scholar
- Mock DW, Parker GA: The Evolution of Sibling Rivalry. 1997, Oxford: Oxford University PressGoogle Scholar
- Osorno JL, Drummond H: Is obligate siblicidal aggression food sensitive?. Behav Ecol Sociobiol. 2003, 54: 547-554. 10.1007/s00265-003-0667-3.View ArticleGoogle Scholar
- Wenseleers T, Hart AG, Ratnieks FLW, Quezada-Euan JJG: Queen execution and caste conflict in the stingless bee Melipona beecheii. Ethology. 2004, 110: 725-736. 10.1111/j.1439-0310.2004.01008.x.View ArticleGoogle Scholar
- Pearcy M, Aron S, Doums C, Keller L: Conditional use of sex and parthenogenesis for worker and queen production in ants. Science. 2004, 306: 1780-1783. 10.1126/science.1105453.View ArticlePubMedGoogle Scholar
- Yamauchi K, Kimura Y, Corbara B, Kinomura K, Tsuji K: Dimorphic ergatoid males and their reproductive behavior in the ponerine ant Hypoponera bondroiti. Insect Soc. 1996, 43: 119-130. 10.1007/BF01242564.View ArticleGoogle Scholar
- Paxton RJ, Weißschuh N, Engels W, Hartfelder K, Quezada-Euan JJG: Not only single mating in stingless bees. Naturwissenschaften. 1999, 86: 143-146. 10.1007/s001140050588.View ArticleGoogle Scholar
- Imperatriz-Fonseca VL, Zucchi R: Virgin queens in stingless bee (Apidae, Meliponinae) colonies: a review. Apidologie. 1995, 26: 231-244. 10.1051/apido:19950305.View ArticleGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.