Development and organization of the larval nervous system in Phoronopsis harmeri: new insights into phoronid phylogeny
© Temereva and Tsitrin; licensee BioMed Central Ltd. 2014
Received: 10 November 2013
Accepted: 9 January 2014
Published: 13 January 2014
The organization and development of the nervous system has traditionally been used as an important character for establishing the relationships among large groups of animals. According to this criterion, phoronids were initially regarded as deuterostomian but have more recently been regarded as protostomian. The resolving of this conflict requires detailed information from poorly investigated members of phoronids, such as Phoronopsis harmeri.
The serotonin-like immunoreactive part of the P. harmeri nervous system changes during larval development. These changes mostly concern the nervous system of the hood and correlate with the appearance of the median and two marginal neurite bundles, the frontal organ, and the sensory field. The apical organ has bilateral symmetry. The tentacular neurite bundle passes under the tentacles, contains several types of perikarya, and gives rise to intertentacular bundles, which branch in the tentacle base and penetrate into adjacent tentacles by two lateroabfrontal bundles. There are two groups of dorsolateral perikarya, which exhibit serotonin-like immunoreactivity, contact the tentacular neurite bundle, and are located near the youngest tentacles. Larvae have a minor nerve ring, which originates from the posterior marginal neurite bundle of the hood, passes above the tentacle base, and gives rise to the mediofrontal neurite bundle in each tentacle. Paired laterofrontal neurite bundles of tentacles form a continuous nerve tract that conducts to the postoral ciliated band.
The organization of the nervous system differs among the planktotrophic larvae of phoronid species. These differences may correlate with differences in phoronid biology. Data concerning the innervation of tentacles in different phoronid larvae are conflicting and require careful reinvestigation. The overall organization of the nervous system in phoronid larvae has more in common with the deuterostomian than with the protostomian nervous system. Phoronid larvae demonstrate some “deuterostome-like” features, which are, in fact, have to be ancestral bilaterian characters. Our new results and previous data indicate that phoronids have retained some plesiomorphic features, which were inherited from the last common ancestor of all Bilateria. It follows that phoronids should be extracted from the Trochozoan (=Spiralia) clade and placed at the base of the Lophotrochozoan stem.
The phylum Phoronida is a small group of marine invertebrates with a biphasic life cycle. Adult phoronids are benthic, worm-like animals, and their larvae, which are called actinotrochs, live in plankton. At both larval and adult stages, phoronids have tentacles, which are used to capture food particles and which exhibit some specific peculiarities in filter-feeding mechanisms (for details see ). Phoronid larvae live in plankton for several months  and then undergo catastrophic metamorphosis [3–5].
The phoronid position among other Bilateria was established by molecular phylogenetic analyses [6, 7]. According to these analyses, phoronids are Trochozoan animals, which together with brachiopods form a clade called the Brachiozoa [8, 9]. According to recent data , phoronids form a group within the brachiopods and are regarded as brachiopods without shells. The protostomian affiliation of phoronids, however, lacks supporting evidence from comparative anatomy and embryology. Moreover, phoronid morphology and embryology have more in common with those of the Deuterostomia than of the Protostomia [11–14]. On the other hand, some recent data revealed that phoronids also have some morphological characters that are not congruent with a strictly deuterostomian interpretation [15–19].
Development and organization of the nervous system has been useful for determining the relationships among different taxa [11, 20, 21]. The use of features of nervous system development and organization of bilaterian larvae has improved phylogenetic interpretation of some bilaterian groups, including: the relationship between segmented annelids and nonsegmented echiurids and sipunculids, which exhibit metamerism of the nervous system in larvae [21–25]; the protostomian affiliation of brachiopods ; and the monophyletic assemblage of Entoprocta + Mollusca . Researchers have several different views regarding the pattern of nervous system organization in phoronid larvae. One view is that phoronid larvae have a deuterostomian-like nervous system . Another view is that the nervous system of phoronid larvae has more in common with the protostomian than with the deuterostomian nervous system . A third view, which is based on the most recent data, is that the organization of the nervous system in phoronid young larvae combines deuterostome- and trochozoan-like features . This disagreement about the organization of the nervous system of phoronid larvae can be partially explained by a lack of breadth in that most studies have been based on Phoronis spp. [15, 27–31] and less frequently on Phoronopsis spp. [18, 19]. In addition, most of the investigations listed above used young phoronid larvae, and detailed data about the organization of the nervous system in competent phoronid larvae are nearly absent . At the same time, some new nerve elements appear in phoronid larvae before metamorphosis. Thus, besides having an apical organ, all competent phoronid larvae have a frontal (or pyriform) organ, which apparently plays a main role in larval settlement . A similar organ is known in bryozoan larvae , but its homology to the phoronid frontal organ is still uncertain. The collection of novel data concerning nervous system organization in phoronid larvae may reveal common patterns and facilitate comparisons with the nervous system of other main groups of Bilateria with ciliary larvae.
Serotonin-like immunoreactive nervous system: overall anatomy and development
The serotonin-like immunoreactive nervous system consists of several elements. The apical organ and the main tentacular neurite bundle (the main nerve ring, the tentacular nerve ring) are the most prominent elements and were found in all larval stages (Figures 1C, G, 2B, D and 3B). During larval development, the nerve elements of the preoral lobe undergo greater changes than the other elements of the serotonin-like immunoreactive nervous system. Neurites and perikarya of the trunk increase in number with age (Figures 2D and 3B). The telotroch nerve ring becomes more prominent with age (Figures 1G, 2B and 3B). In inner organs, serotonin-like immunoreactive elements occur in the cardial sphincter and are represented by circular neurites, which form a ring between the esophagus and the stomach (Figure 2C). The metasomal sac is also innervated by numerous serotonin-like immunoreactive neurites and perikarya (Figure 3C).
The apical organ occupies the epidermis of the apical plate and consists of perikarya and neurites of different types. In young larvae with 18-20 tentacles, the apical organ is composed of 20-25 perikarya with cilia and 20 other perikarya that do not contact the surface of the apical plate and that do not bear cilia (Figure 1B, C). Flask-shaped perikarya with cilia are arranged in a horseshoe-like pattern along the anterior and lateral edge of the apical plate. The branches of the horseshoe-like structure are directed toward the dorsolateral sides. The flask-shaped perikarya have basal processes, which bear several varicosities (nodes) and pass to the center of the apical plate and form the neuropil (Figure 1E). Perikarya that do not contact the surface of the apical plate are located under the neuropil and are arranged in two lateral groups. As a consequence, the central neuropil is divided into upper and lower portions in transversal optical sections (Figure 1B). The neuropil contacts the basal lamina along the sagittal line but is separated from the basal lamina by underlying perikarya in other areas. The number of ciliated perikaria increases with age and reach 30 in larvae with 22 tentacles (Figure 2C, F) and 37 in competent larvae (Figure 3G). In competent larva, the apical organ includes ciliated flask-shaped perikarya and two groups of underlying perikarya (Figure 3H).
The main tentacular neurite bundle is the most prominent element of the serotonin-like nervous system of P. harmeri at all larval stages. In young larvae, the tentacular neurite bundle contains a few perikarya, which with immunocytochemical staining are recognizable on the dorsolateral sides in the base of the youngest tentacles (Figure 1C, G). Here two types of perikarya were revealed by TEM (see below).
The tentacular neurite bundle originates from the lower part of the neuropil of the apical organ and is split into two dorsolateral branches (Figure 1B), which run under the tentacles along the lateral sides of the body and meet on the ventral side. For this reason, the tentacular neurite bundle has been called the “main nerve ring” . Each dorsolateral branch extends from the apical organ as by several neurites, which maintain close contact with each other on the dorsal side and split into numerous thin neurites in the branch points, where the youngest tentacles are located (Figure 1G). At all larval stages studied here, each dorsolateral branch of the main tentacular nerve splits into two groups of neurites (Figure 2E). Neurites of the first group form a net under the base of the tentacles. Individual neurites originate from this net and penetrate into each tentacle. Intertentacular branches are usually present, and these bifurcate in the base of the tentacle and form two branches that extend into adjacent tentacles (Figure 2J). Thus, each tentacle contains two lateroabfrontal serotonin-like immunoreactive neurites, which originate from different intertentacular branches (Figures 1C, H and 3F). In young tentacles, neurites form distal varicosities (Figure 1H and J). Neurites of the second group are more numerous and prominent than neurites of the first group. Neurites of the second group spread along the lateral and ventral sides of the trunk (Figure 2E). These neurites are associated with serotonin-like immunoreactive perikarya, which are scattered in the epidermis of the trunk (Figure 2D).
The telotroch is innervated by a serotonin-like immunoreactive neurite bundle, which is associated with neurites of the trunk and is located in the truncal epidermis adjacent to the epidermis of the telotroch (Figures 1G, 2B and 3B). This neurite bundle forms a circle above the epidermis of the telotroch.
The median neurite bundle develops from the neuropil of the apical organ and passes to the edge of the preoral lobe. In young larvae with 18-20 tentacles, the median neurite bundle consists of two serotonin-like immunoreactive branches (right and left), each of which bends near the preoral lobe edge and passes along it as portion of the posterior marginal neurite bundle (Figures 1G, J and 4C). Each branch ends on the lateral side of the preoral hood, where several serotonin-like immunoreactive perikarya are located and are associated with the neurite bundle (Figure 1G and J). Interestingly, in young larvae with 18-20 tentacles, staining against alpha-tubulin reveals three branches of the median neurite bundle (instead of two serotonin-like immunoreactive branches) and two marginal neurite bundles: a posterior marginal neurite bundle, which has serotonin-like immunoreactivity, and an anterior marginal neurite bundle, which lacks serotonin-like immunoreactivity (Figure 1F).
Among larvae with 22 tentacles, different degrees of complexity are evident in the serotonin-like immunoreactive elements of the preoral lobe. First, the central branch of the median neurite bundle becomes serotonin-like immunoreactive (Figures 2C, F and 4D). This branch originates from the apical organ and passes to the posterior marginal neurite bundle, but does not reach it and is not evident in the most distal end of the hood (Figure 2F). At the same time, staining against alpha-tubulin shows that the distal end of the medial branch forms a bulge, which contacts the posterior marginal neurite bundle (Figure 2G). Serotonin-like immunoreactive pekirarya, which are associated with the posterior marginal neurite bundle, increase in number to 10–13 on each side of the hood (Figure 2C, D and F). In the next step of development, the serotonin-like immunoreactive anterior marginal bundle of the preoral lobe appears (Figure 2B). This neurite bundle passes along the distal edge of the preoral lobe, conducts the annular muscle of the preoral lobe, and passes to the ventrolateral sides of the oral field (Figure 2B).
In competent larvae, the organization of the median neurite bundle is more complicated than in earlier stages. The distal end of the median branch of the median neurite bundle is not recognizable by staining with serotonin. For this reason, the distal end of the median neurite bundle looks like a loop in Z-projections (Figures 3B, E and 4E). The empty space of this loop is occupied by the enlarged end of the median branch, which does not exhibit serotonin-like immunoreactivity but which is revealed by staining with alpha-tubulin (Figure 3D). The distal ends of lateral branches in the median neurite bundle bear numerous flask-shaped cells, which contact the epidermis surface and are probably sensory (Figures 3E and 4E). Perikarya of the posterior marginal neurite bundle increase in number and before metamorphosis form a sensory field along the center of the preoral lobe edge (Figure 2B). This sensory field consists of numerous flask-shaped sensory cells and perikarya, which do not contact the surface of the epidermis (Figure 3B and E). Because the distal edge of the preoral lobe is partially tucked in a vestibulum (Figure 3A), the anterior marginal neurite bundle seems located behind the posterior marginal neurite bundle in Z-projections (Figure 3D). Right and left branches of the anterior marginal neurite bundle continue to the oral field. The posterior marginal neurite bundle is very thick in the center of the preoral lobe but very thin on the lateral and dorsolateral sides (Figure 3B).
In competent larva, the following serotonin-like immunoreactive elements are evident: the apical organ, the tentacular neurite bundle associated with perikarya, paired lateroabfrontal neurite bundles in each tentacle, neurites and perikarya of the trunk, perikarya and neurites in the epidermis of the metasomal sac, the telotroch neurite bundle, median neurite bundles of the preoral lobe, the anterior marginal neurite bundle with ventrolateral protrusions, the posterior marginal nerve associated with serotonin-like immunoreactive sensory cells, and neurites of the cardial sphincter (Figure 4A).
FMRFamide-like immunoreactive nervous system
FMRFamide-like immunoreactive neurites and perikarya were found in different organ systems of the competent larvae. Thin longitudinal and circular neurites form a net around the esophagus and the mouth (Figure 5F). Longitudinal neurites were found in the epidermis of the metasomal sac (Figure 5G). Thin circular neurites innervate the metasomal sac opening (Figure 5G). The epithelium of the midgut, contains about 40 FMRFamide-like immunoreactive perikarya (Figure 5K). These are flask-shaped cells that contact the gut lumen; their basal processes form a net around the midgut. The anus is innervated by a circular neurite bundle, which is located in the epithelium on the border between the proctodaeum and the epidermis of the body (Figure 5E).
alpha-tubulin-like immunoreactive elements
In competent larvae some nerve elements do not exhibit serotonin-like or FMRFamide-like immunoreactivity but can be revealed by staining with alpha-tubulin. The minor nerve ring gives rise to the mediofrontal neurite bundle in each tentacle (Figure 5H-J). Staining with alpha-tubulin facilitates the observation of the laterofrontal neurite bundles in each tentacle (Figure 5J). They contact between the tentacles and form a continuous nervous tract that conducts the postoral ciliated band. Thus, the innervation of tentacles is provided by five longitudinal neurite bundles: one mediofrontal, two laterofrontal, and two lateroabfrontal. The sensory cells of the tentacle also can be found by staining with alpha-tubulin. These cells are located along the laterofrontal sides of each tentacle. The sensory cells are usually grouped in pairs (Figure 5H-J).
Transmission electron microscopy was used to reveal the fine organization of the main nervous system elements. The nature of nerve elements that are 5HT or FMRFamide reactive cannot be recognized, and here we only show the fine organization of the perikarya and neurites, their position with respect to each other, and their location with respect to other organs and tissues.
The sensory field is formed by several types of perikarya that are associated with the posterior marginal neurite bundle. Sensory cells and perikarya, which do not contact the epidermis surface, occur along the middle portion of the posterior marginal neurite bundle (Figure 8B and D). Sensory cells are large, bear a cilium, and have striated rootlets in the cytoplasm (Figure 8D). The basal part of sensory cells forms several processes, which are filled with synaptic vesicles (Figure 8B and D). Perikarya that do not contact the surface of the epidermis are located near the sensory cells and contain dense-core synaptic vesicles (Figure 8D). Some cells of the hood edge are biciliar and contain two basal bodies (Figure 8C).
The midgut contains many cells, which have an unusual organization relative to that of the regular epithelial cells (Figure 10D). Each of these unusual cells contains a large, roundish nucleus with electron-lucent karyoplasm and a large nucleolus. The cell cytoplasm is filled with numerous electron-dense granules, with diameters ranging from 100 to 115 nm. The same granules were found in neurites, which are visible in the basal portion of the midgut epithelium (Figure 10D).
In the epidermis of the oral field, small bundles of neurites and perikarya are evident with TEM. The perikarya contain dense-core vesicles. The neurites are filled with large, dense-core vesicles and vesicles with medium-dense content (Figure 10E).
The telotroch nerve ring is located in the epidermis between the trunk wall and telotroch (Figure 10F). It consists of 5-7 neurites of different diameters. Large neurites have light cytoplasm and contain clear synaptic vesicles. Small neurites contain dense-core vesicles.
Organization of the nervous system in competent larvae
Taken together, our results reveal that the nervous system in competent larvae of P. harmeri consists of the following elements: an apical organ, a median neurite bundle, an anterior and posterior marginal neurite bundle, a frontal organ and a sensory field, a tentacular neurite bundle (main nerve ring), two dorsolateral groups of perikarya, a minor nerve ring, five radial neurite bundles in each tentacle, a telotroch nerve ring, an anal nerve ring, neurites and perikarya in the epidermis of the oral field, the trunk, an esophagus, a metasomal sac, and a midgut (Figure 4B).
Changes of phoronid nervous system anatomy during larval development
The nervous systems of phoronid larvae have been well studied by a variety of methods, including light microscopy , TEM [14, 28, 30], immunocytochemistry [27, 29], and confocal laser scanning microscopy [16, 18, 19, 31]. Previous studies usually involved precompetent larvae, but the nervous system of phoronid larvae changes greatly before metamorphosis. According to our results, these changes mostly concern the preoral lobe of the larva, which apparently plays a main role in larval settlement . The organization of the median neurite bundle becomes very complicated with age. At all larval stages, it consists of two lateral and one median bundles, but only lateral bundles exhibit serotonin-like immunoreactivity in precompetent larvae. Interestingly, although the median bundle in competent larvae exhibits serotonin-like immunoreactivity, the distal end of this bundle does not display this activity. At the same time, the distal portion of the median bundle exhibits FMRFamide-like immunoreactivity. The frontal organ appears in the distal portion of the median neurite bundle. This is the second nerve center, which appears before metamorphosis in all phoronid larvae . In some phoronid larvae, the frontal organ sticks out from the epidermis surface, whereas in other larvae, it is not everted above the epidermis surface and is not visible in live larvae . In competent larvae of P. harmeri, the frontal organ is not everted and can be recognized in sections. The serotonin-like immunoreactive part of the frontal organ includes two groups of perikarya: frontal and median. The aggregation of perikarya near the edge of the midline of the hood was found in some competent phoronid larvae , whereas the frontal perikarya, which form a huge group (a sensory field), are described for the first time in the current report. In young larvae of P. muelleri, several serotonin-like immunoreactive monopolar perikarya occur along the median part of the epistome edge, but their connection with median and marginal neurite bundles is unclear .
Perikarya of the sensory field are associated with the anterior neurite bundle, which exhibits serotonin-like immunoreactivity in young  and in competent (herein) larvae of P. harmeri but not in precompetent larvae of P. harmeri. The plasticity of the timing when some nerve elements repeatedly appear and disappear during phoronid development was previously mentioned [18, 19, 29]. This plasticity also relates to the distribution of neurites and perikarya of the oral field of the larvae. Interestingly, the oral field, which is well innervated in young larvae of P. harmeri[18, 19], is nearly without immunoreactive perikarya and neurites in competent larvae. Competent larvae have a single nerve ring of the telotroch, whereas the second (posterior) nerve ring, which is evident in young larvae , cannot be found by immunocytochemistry or TEM.
The other changes of the larval nervous system concern the increase in the number of perikarya in the apical organ, in the midgut, and in the epidermis of the trunk.
Additional file 1: Movie 1: The competent larva of Phoronopsis harmeri. This time-lapse movie shows the typical behavior of competent larvae before settlement. The larva used a piece of the tube of an adult P. harmeri as a substratum for metamorphosis. In the movie, the piece of the tube is the white stone to the left. The larval hood always moves and touches the piece of tube. At this moment, the hood elongates along midline, and then the larva begins to metamorphose. (AVI 6 MB)
Nervous system of phoronid larvae
Among phoronid larvae, two types of nervous system organization have been recognized . According to Santagata and Zimmer, “Type 1 species have two fiber-rich, dorsal serotonergic nerves that extend from the apical ganglion and are continuous with the main nerve ring. This type also has two lateral serotonergic processes that form part of the minor nerve ring and the abfrontal nerves of the larval tentacles. Catecholaminergic fibers from the main hood nerve are continuous with the frontal larval tentacle nerves. Type 2 species have two fiber-poor, dorsal serotonergic nerves that extend from the apical ganglion and are continuous with the main nerve ring. These species exhibit a fiber-rich, serotonergic, and catecholaminergic main hood nerve from which all fibers of the minor nerve ring and larval tentacle nerves originate” .
The organization of the nervous system of P. harmeri larvae, however, does not fit either type. It differs from type 1 in its lack of the “lateral serotonergic processes”. These paired processes extend from the apical organ and contact the minor nerve ring, which gives rise to the abfrontal nerves of the tentacles . The nervous system of P. harmeri larvae is more similar to the type 2 than type 1 nervous system, but differs from the type 2 in the innervation of the tentacles. In larvae with the type 2 nervous system, all tentacular nerves originate from the minor nerve ring, whereas only the mediofrontal tentacular nerves originate from the minor nerve ring in P. harmeri larvae.
The innervation of the tentacles in phoronid larvae is controversial. According to Hay-Schmidt’s immunocytochemistry data , young larvae of P. muelleri have six neurite bundles in each tentacle. One mediofrontal neurite bundle consists only of catecholamine-containing neurites. Two laterofrontal neurite bundles exhibit catecholamine- and FMRFamide-like immunoreactivity. Two lateroabfrontal neurite bundles consist of catecholamine- and serotonin-like immunoreactive neurites. One medioabfrontal neurite bundle exhibits serotonin-like immunoreactivity. The medioabfrontal neurite bundle originates from two groups of “lateral epistome-mesosome processes”, which extend from the neuropile towards the ventrolateral side of the oral field where they branch once or twice before one branch extends into the tentacle. These branches do not skirt the tentacles (see Figure 3A,B in ), and their location on the abfrontal side of tentacle is unclear, because the epidermis of the oral field continues to the frontal but not to the abfrontal side of the tentacle. Because the neurites are intraepidermal, they must follow the epidermis; for innervation of the abfrontal side of tentacles, neurites of the oral field must skirt the tentacle bases. At the same time, according to Hay-Schmidt’s ultrastructural data , larva of P. muelleri has one mediofrontal, two laterofrontal, and two small lateroabfrontal neurite bundles in each tentacle.
On the other hand, according to Santagata and Zimmer , some phoronid larvae (those with the type 1 nervous system) lack neurites along the frontal side of tentacles. Moreover, these authors suggested that the lateroabfrontal neurites originate from the minor nerve ring, whereas our observations and those of Hay-Schmidt  indicate that these neurites originate from the main nerve ring.
The question of how the tentacles are innervated in phoronid larvae requires additional investigation because comparative analysis of tentacle innervation is important for distinguishing among groups of “lophophorates”. Our results indicate that the innervation of the larval tentacles involves both the main and minor nerve ring.
In general, the serotonin-like immunoreactive nervous system is more complicated in competent larvae of P. harmeri than in other phoronid larvae studied to date. First, competent larvae of P. harmeri have two serotonin-like immunoreactive marginal neurite bundles (the anterior bundle, which continues to the oral field, and the posterior bundle, which is associated with serotonin-like immunoreactive cells of two types and gives rise to the minor nerve ring); other phoronid larvae have only one marginal neurite bundle (the posterior bundle) [16, 27]. Second, competent larvae of P. harmeri have a “sensory field” on the edge of the hood midline. Third, competent larvae of P. harmeri have numerous neurites and perikarya along the larval trunk and the telotroch nerve ring. These nerve elements have been observed in P. muelleri larvae  but not in other phoronid larvae studied to date [16, 30]. Fourth, competent larvae of P. harmeri have perikarya that are scattered along the main nerve ring and form two dorsolateral groups in the sites of the youngest tentacles. Fifth, among all phoronid larvae studied to date, the innervation of inner organs has been observed only in P. harmeri. The innervation of the esophagus and cardiac sphincter in P. harmeri larvae is provided by both serotonin-like and FMRFamide-like immunoreactive neurites , herein. The small number of FMRFamide-like immunoreactive perikarya that occur in the midgut of young larvae of P. harmeri, increase to as many as 30 in competent larvae.
Our ultrastructural data provide new information about the organization of the nervous system in phoronid larvae. In general, these data corroborate the results of Hay-Schmidt , Lacalli , and Santagata . The apical organ consists of several types of perikarya. According to the location of the perikarya of the apical organ, we can infer that they belong to the serotonin-like immunoreactive sensory cells (perikarya of the first type), serotonin-like immunoreactive cells under the neuropil (perikarya of the second type), and FMRFamide-like immunoreactive non-sensory cells (perikarya of the third type). According to Santagata’s data , the organization of the apical organ is simpler for competent larvae of P. pallida than for P. muelleri or P. harmeri, herein competent larvae. Thus, the apical organ of P. pallida lacks non-sensory serototin-like immunoreactive cells, which are located under the neuropil in the apical organs of P. muelleri and P. harmeri. Interestingly, the apical organ of young larvae of P. vancouverensis also lack perikarya under the neuropil . The differences in the organization of the apical organ can be explained by differences in larval biology: P. pallida and P. vancouverensis larvae live in plankton for only 1 month, whereas P. harmeri larvae live in plankton for 3 months.
The organization of the frontal organ seems similar in competent larvae of both P. harmeri and P. pallida. In P. pallida larvae, the frontal organ has a large neuropil, which is located close to the apical organ but does not contain any serotonin- or catecholamine-like immunoreactive perikarya. At the same time, TEM reveals sensory and nonsensory cells around the neuropil of the frontal organ of P. pallida. In P. harmeri larvae, the frontal organ is distant from the apical organ and contains several types of perikarya, which were revealed with both TEM and immunocytochemical methods.
The organization of the nervous system of the larval hood differs among the studied species. Thus, larvae of three species (P. harmeri, P. muelleri, and P. vancouverensis) have both anterior and posterior marginal neurite bundles, whereas actinotrocha C, actinotrocha D, and larvae of P. harmeri from the Pacific Coast of North America have only a single marginal neurite bundle . The marginal neurite bundles in P. muelleri larvae do not exhibit serotonin-like immunoreactivity as they do in P. harmeri larvae. At the same time, the posterior marginal neurite bundle is located very distant from the hood edge in P. muelleri larvae  but is located much closer to the hood edge in larvae of P. vancouverensis and P. harmeri [herein]. In young larvae of P. vancouverensis, “glial-like capsular” cells are associated with the posterior marginal neurite bundle.
TEM revealed that the innervation of tentacles is more complicated in P. harmeri larvae than in other phoronid larvae [28, 30]. Besides the longitudinal neurite bundles, which occur in the tentacles of other phoronid larvae, P. harmeri larvae have additional sensory cells that accompany the laterofrontal sensory cells and the non-sensory perikarya associated with the mediofrontal neurite bundle. The ultrastructural organization of the laterofrontal sensory cells in the tentacles of P. harmeri larvae seems very similar to that for adult phoronids .
The oral field is well innervated in young phoronid larvae [18, 19, 27, 29, 30]. At the same time, in competent larvae, the neurites and perikarya of the oral field do not exhibit immunoreactivity [16, 31], herein and can be detected only by TEM [herein].
In P. harmeri larvae, the main tentacular neurite bundle contains many perikarya of different types, and synaptic contacts occur between the neurites. Perikarya have never been found in the tentacular neurite bundle of other phoronid larvae [16, 27–31], perhaps because the studied larvae were noncompetent [27–30] and the nervous system organization of noncompetent larvae is likely to be simple. This difference in the organization of the tentacular neurite bundle may reflect the general difference in the organization of the whole nervous system among different phoronid larvae.
The innervation of the internal organs was described previously , and our recent data are consistent with these earlier findings. The ultrastructure of perikarya in the midgut seems very similar in both P. harmeri larvae and actinotrocha sp. . These cells lack synaptic vesicles and are filled with electron-dense granules, whose presence is a hallmark of neurosecretory cells . Neurosecretory cells are common in the digestive epithelium, where they control the movement of food.
Organization of the nervous system in Brachiozoa
As mentioned earlier, phoronids are regarded as the closest relatives of brachiopods [8–10]. Phoronids and brachiopods exhibit at least two types of development: planktotrophic and lecithotrophic. The nervous system is very complex (consists of many different elements) for planktotrophic larvae [16, 38] but is usually very simple for lecithotrophic larvae and consists only of an apical organ with a small number of cells and a pair of longitudinal neurite bundles [26, 39]. Two important questions arise: Which type of development arose first in the Brachiozoa: planktotrochic or lecithotrophic? Which type of nervous system is plesiomorphic among the Brachiozoa? According to paleontological data , planktotrophy may be regarded as the primary condition of brachiopods. For this reason, we suggest that the larvae of the last common brachiozoan ancestor had tentacles that were used to feed. Therefore, the presence of planktotrochic larvae with nervous systems consisting of many elements may be regarded as the primary condition of the Brachiozoa.
Serotonin-like immunoreactive nervous system in bilaterian ciliated larvae
Features of the organization of the serotonin-like immunoreactive nervous system in ciliated larvae of phoronids and in some protostomian and deuterostomian organisms
Nemertea, Polychaeta, Mollusks
location where the first perikarya appear
always on the anterior pole of the embryo
mostly on the anterior pole, but on the posterior pole of the embryo in some species
always on the anterior pole of the embryo
location where the first neurites appear
neurites under the postoral ciliated band
neurites under the preoral ciliated band
neurites under the postoral ciliated band
number of perikarya in the apical organ
(more than 50)
(more than 30)
organization of the neurite bundle underlying the postoral ciliated band:
1)― (in the most of protostomia larvae) + 
2) presence of the connection with apical organ
2) ― (even if neurite bundle exists)
3) presence of perikarya among neurites
3) ― (even if neurite bundle exists)
presence of the telotroch nerve ring
presence of the ventral nerve cord in early development
The nervous system in bilaterian ciliated larvae and the ground plan of the bilaterian nervous system
There is a classic controversy in zoology about whether the common ancestor of living bilateria was a benthic animal with a bilaterian body plan or a pelagic larva-like animal similar to what we see today in the primary larvae of indirect-developing bilaterians (for review, see [54, 55]). Although most recent authors believe that the feeding larvae are specializations of the ontogeny of an ancestral, direct development [56–58], the organization of ciliated larvae is still useful for evolutionary and phylogenetic deductions [20, 21], especially with respect to the development and organization of the nervous and muscular systems. According to modern analysis [59–61], the last common bilaterally symmetrical ancestor had a centralized nervous system, which consisted of a nerve center and nerve cords that innervated the tentacles. The nerve center had a simple histological organization, consisting of perikarya and a neuropil, and did not contain any additional (glial) cells . This organization is evident in the larvae of many extant bilaterian groups. The organization of the nerve center of the last bilaterian ancestor may have consisted of many or only a few cells. Nerve centers with many cells occur in larvae of the following extant bilaterian: phoronids , deuterostomians [41, 42], some mollusks , and entoprocts . Whereas those bilaterians having nerve centers consisting of many cells belong to two different stems of Bilateria (Protostomia and Deuterostomia), those with nerve centers consisting of only a few cells occur only in the Protostomia. For this reason, it seems logical to infer that the last common bilaterian ancestor had a nerve center consisting of many cells and that the cell number was reduced in some groups as a result of lecithotrophy (as in some brachiopods ) or determination of early development (as in Spiralia).
Based on all of these data, we suggest that phoronid larvae have retained some features of the ancestral nervous system and can be regarded as more primitive than their spiralian “relatives”. It follows that phoronids should be extracted from the Trochozoan (=Spiralia) clade and placed at the base of the Lophotrochozoan stem. This idea was first suggested by Peterson and Eernisse  and has been confirmed by other results [1, 5, 14, 63].
The organization of the nervous system is more complicated in the competent larvae of P. harmeri than in any other phoronid larvae studied to date. Before metamorphosis, the nervous system P. harmeri larvae includes the bilaterally symmetrical apical organ, the tripartite median neurite bundle with frontal organ and a sensory field on the distal end, the anterior and posterior marginal neurite bundles, two dorsolateral groups of perikarya, main and minor nerve rings, five radial neurite bundles in each tentacle, trunk neurites and perikarya, the telotroch nerve ring, and perikarya and neurites of the esophagus, the midgut, and the metasomal sac. The organization of the nervous system differs among phoronid larvae, and these difference reflect the presence of different types of phoronid planktotrophic larvae [2, 16]. Comparative analysis of the nervous system organization in lophotrochozoan and deuterostomian larvae revealed that the overall anatomy of the nervous system of phoronid larvae has more in common with the deuterostomian pattern than with protostomian pattern. The most important distinguishing characteristic is that phoronid and deuterostomian larvae but not protostomian larvae have a bilaterally symmetrical apical organ that contains many serotonin-like immunoreactive perikarya and that gives rise to the prominent neurite bundle passing along the postoral ciliated band. At the same time, the presence of the apical organ and the nerve cords, which innervate the tentacles, is the most plesiomorphic condition of the bilaterian nervous system. For this reason, phoronids can be regarded as primitive lophotrochozoans.
Advanced and competent larvae of P. harmeri were collected with a planktonic net during November of 2011 in Vostok Bay, Sea of Japan (for details see . Larvae were reared at 1 to 3°C in an incubator with a 12-h light–dark cycle until metamorphosis.
Advanced and competent larvae were photographed using a Panasonic DMC-TZ10 digital camera mounted on a binocular light microscope. All larval stages were prepared for scanning electron microscopy (SEM), transmission electron microscopy (TEM), cytochemistry, and confocal laserscanning microscopy (CLSM).
For SEM, fixed larvae of P. harmeri that had been dehydrated in ethanol followed by an acetone series were critical point dried and then sputter coated with platinum-palladium alloy. Specimens were examined with a Jeol JSM scanning electron microscope.
For TEM, larvae of P. harmeri were fixed at 4°C in 2.5% glutaraldehyde in 0.05 M cacodylate buffer containing 21 mg/ml NaCl and then postfixed in 2% osmium tetroxide in the same buffer containing 23 mg/ml NaCl. Postfixation was followed by en bloc staining for 2 h in a 1% solution of uranyl acetate in distilled water. Specimens were then dehydrated in ethanol followed by an acetone series and embedded in Spurr resin (Sigma Aldrich). Semi-thin and thin sections were cut with a Reichert Ultracut E ultratome. Semi-thin sections were stained with methylene blue, observed with Zeiss Axioplan2 microscope and photographed with an AxioCam HRm camera. Thin sections were stained with lead citrate and then examined with a JEOL JEM 100B electron microscope.
For cytochemistry, advanced and competent larvae of P. harmeri were narcotised in MgCl2, then fixed overnight in a 4% paraformaldehyde solution on a filtrate of sea water and washed (two times) in phosphatic buffer (pH 7.4) (Fisher Scientific) with Triton X-100 (0.3%) (Fisher Scientific, Pittsburgh, PA, USA) for a total of 2 h. Nonspecific binding sites were blocked with 1% normal donkey serum (Jackson ImmunoResearch, Newmarket, Suffolk, UK) in PBT overnight at +4°C. Subsequently, the larvae were transferred into primary antibody: the mixture of a-Acetylated Tubulin (1:1000) and either anti-FMRFamide (1:3000) or anti-serotonin (1:1000) (ImmunoStar, Hudson, WI, USA) in PBT and incubated for 24 h at +4°C with gentle rotation. Specimens were washed for 8 h at +4°C (at least three times) in PBT and then exposed to the secondary antibody: donkey anti-rabbit- Atto 647 N and donkey anti-mouse-Atto 565 (Invitrogen, Grand Island, NY, USA) both 2-3 mkg/ml in PBT for 24 h at +4°C with gentle rotation. In the following, they were washed in PBS (three times × 40 min), mounted on a cover glass covered with poly-L-lysine (Sigma-Aldrich, St. Louis, MO, USA), and embedded in Murray Clear. Specimens were viewed with Leica TCS SP5 confocal microscope (IDB, Moscow, Russia). Z-projections were generated using the programme Image J version 1.43. Three-dimensional reconstructions were generated using Amira version 5.2.2 software (Bitplane, Zurich, Switzerland).
In this report, we have used the terms suggested by Russel Zimmer , who used light microscopy to provide the first complete description of the nervous system of phoronid larvae. We have also used some terms suggested by Scott Santagata [16, 31] and specific neuroscience terms proposed by Richter and colleagues .
This research was supported in part by grants to ET from the Russian Foundation for Basic Research (# 14-04-00238; # 12-04-33045) and Grant of the President of Russia (# MD-1979.2013.4). The work was performed at User Facilities Center of M.V. Lomonosov Moscow State University under financial support of Ministry of Education and Science of Russian federation. ET is very grateful to her friend Svetlana Maslakova for hosting her at the Oregon Institute of Marine Biology in 2010 and for providing resources and training, especially in immunohistochemistry and confocal microscopy. ET is grateful to Thomas Schwaha and Stephan Handschuh for helping her with the Amira program. We thank B. Jaffee for help with the English language.
- Strathmann R: Ciliary sieving and active ciliary response in capture of particles by suspension-feeding brachiopod larvae. Acta Zool. 2005, 86: 41-54. 10.1111/j.0001-7272.2005.00185.x.View ArticleGoogle Scholar
- Temereva EN: New data on distribution, morphology and taxonomy of phoronid larvae (Phoronida, Lophophorata). Invert Zool. 2009, 6 (1): 47-64.Google Scholar
- Kovalevsky АО: Anatomy and life history of Phoronis. Proc St Petersburg Acad Sci. 1867, 2: 1-35.Google Scholar
- Herrmann K: Larvalentwicklung und Metamorphose von Phoronis psammophila (phoronida, Tentaculata). Helgol Wiss Meeresuntersuch. 1979, 32: 550-581. 10.1007/BF02277994.View ArticleGoogle Scholar
- Temereva EN, Tsitrin EB: Development, organization, and remodeling of phoronid muscles from embryo to metamorphosis (Lophotrochozoa: Phoronida). BMC Dev Biol. 2013, 13: 14-10.1186/1471-213X-13-14. doi:10.1186/1471-213X-13-14PubMedPubMed CentralView ArticleGoogle Scholar
- Dunn CW, Hejnol A, Matus DQ, Pang K, Browne WE, Smith SA, Seaver E, Rouse GW, Obst M, Edgecombe GD, Sørensen MV, Haddock SD, Schmidt-Rhaesa A, Okusu A, Kristensen RM, Wheeler WC, Martindale MQ, Giribet G: Broad phylogenomic sampling improves resolution of the animal tree of life. Nature. 2008, 452: 745-749. 10.1038/nature06614. doi:10.1038/nature06614PubMedView ArticleGoogle Scholar
- Paps J, Baguñà J, Riutort M: Lophotrochozoa internal phylogeny: new insights from an up-to-date analysis of nuclear ribosomal genes. Proc R Soc. 2009, 276: 1245-1254. 10.1098/rspb.2008.1574. doi:10.1098/rspb.2008.1574View ArticleGoogle Scholar
- Cohen B, Weydmann A: Molecular evidence that phoronids are a subtaxon of brachiopods (Brachiopoda: Phoronata) and that genetic divergence of metazoan phyla began long before the early Cambrian. Organ Divers Evol. 2005, 5: 253-273. 10.1016/j.ode.2004.12.002.View ArticleGoogle Scholar
- Santagata S, Cohen B: Phoronid phylogenetics (Brachiopoda; Phoronata): evidence from morphological cladistics, small and large subunit rDNA sequences, and mitochondrial cox1. Zool J Linn Soc. 2009, 157: 34-50. 10.1111/j.1096-3642.2009.00531.x.View ArticleGoogle Scholar
- Cohen BL: Rerooting the rDNA gene tree reveals phoronidsto be 'brachiopods without shells’; dangers ofwide taxon samples in metazoan phylogenetics (Phoronida; Brachiopoda). Zool J Linn Soc. 2013, 167: 82-92. 10.1111/j.1096-3642.2012.00869.x.View ArticleGoogle Scholar
- Hay-Schmidt A: The evolution of the serotonergic nervous system. Phil Trans R Soc B. 2000, 267: 1071-1079.Google Scholar
- Temereva EN, Malakhov VV: Embryogenesis and larval development of Phoronopsis harmeri Pixell, 1912 (Phoronida): dual origin of the coelomic mesoderm. Invert Reprod Dev. 2007, 50: 57-66.View ArticleGoogle Scholar
- Temereva EN, Malakhov VV: Organization of the epistome in Phoronopsis harmeri (Phoronida) and consideration of the coelomic organization in Phoronida. Zoomorphol. 2011, 130: 121-134. 10.1007/s00435-011-0126-z.View ArticleGoogle Scholar
- Temereva EN, Malakhov VV: Embryogenesis in phoronids. Invert Zool. 2012, 9 (1): 1-39.Google Scholar
- Santagata S: Larval development of Phoronis pallida (Phoronida): implications for morphological convergence and divergence among larval body plans. J Morphol. 2004, 259: 347-358. 10.1002/jmor.10205.PubMedView ArticleGoogle Scholar
- Santagata S, Zimmer RL: Comparison of the neuromuscular system among actinotroch larvae: systematic and evolutionary implication. Evol Dev. 2002, 4: 43-54. 10.1046/j.1525-142x.2002.01056.x.PubMedView ArticleGoogle Scholar
- Pennerstorfer M, Scholtz G: Early cleavage in Phoronis muelleri (Phoronida) displays spiral features. Evol Dev. 2012, 14 (6): 484-500. 10.1111/ede.12002.PubMedView ArticleGoogle Scholar
- Temereva EN: Ventral nerve cord in Phoronopsis harmeri larvae. J Exp Zool (Mol Dev Evol). 2012, 318B: 26-34. 10.1002/jez.b.21437.View ArticleGoogle Scholar
- Temereva E, Wanninger A: Development of the nervous system in Phoronopsis harmeri (Lophotrochozoa, Phoronida) reveals both deuterostome- and trochozoan-like features. BMC Evol Biol. 2012, 12: 1-27. 10.1186/1471-2148-12-1.View ArticleGoogle Scholar
- Wanninger A: Shaping the things to come: ontogeny of lophotrochozoan neuromuscular systems and the Tetraneuralia concept. Biol Bull. 2009, 216: 293-306.PubMedGoogle Scholar
- Wanninger A: Comparative lophotrochozoan neurogenesis and larval neuroanatomy: recent advances from previously neglected taxa. Acta Biol Hung. 2008, 59: 127-136. 10.1556/ABiol.59.2008.Suppl.21.PubMedView ArticleGoogle Scholar
- Hessling R, Westheide W: Are Echiura derived from a segmented ancestor?–immunohistochemical analysis of the nervous system in developmental stages of Bonellia viridis. J Morph. 2002, 252: 100-113. 10.1002/jmor.1093.PubMedView ArticleGoogle Scholar
- Kristof A, Wollesen T, Wanninger A: Segmental mode of neural patterning in sipuncula. Curr Biol. 2008, 18: 1129-1132. 10.1016/j.cub.2008.06.066.PubMedView ArticleGoogle Scholar
- Kristof A, Wollesen T, Maiorova AS, Wanninger A: Cellular and muscular growth patterns during sipunculan development. J Exp Zool (Mol Dev Evol). 2011, 316: 227-240.View ArticleGoogle Scholar
- Wanninger A, Kristof A, Brinkmann N: Sipunculans and segmentation. Comm Integr Biol. 2009, 2: 56-59. 10.4161/cib.2.1.7505.View ArticleGoogle Scholar
- Altenburger A, Wanninger A: Neuromuscular development in Novocrania anomala: evidence for the presence of serotonin and a spiralian-like apical organ in lecithotrophic brachiopod larvae. Evol Dev. 2010, 12 (1): 16-24. 10.1111/j.1525-142X.2009.00387.x.PubMedView ArticleGoogle Scholar
- Hay-Schmidt A: Distribution of catecholamine containing, serotonin-like and neuropeptide FMRFamide-like immunoreactive neurons and processes in the nervous system of the actinotroch larva of Phoronis muelleri (Phoronida). Cell Tiss Res. 1990, 259: 105-118. 10.1007/BF00571435.View ArticleGoogle Scholar
- Hay-Schmidt A: The nervous system of the actinotroch larva of Phoronis muelleri (Phoronida). Zoomorphol. 1989, 108: 333-351. 10.1007/BF00312274.View ArticleGoogle Scholar
- Hay-Schmidt A: Catecholamine-containing, serotonin-lake and FMRFamide-like immunoreactive neurons and processes in the nervous system of the early actinotroch larva of Phoronis vancouverensis (Phoronida): distribution and development. Can J Zool. 1990, 68 (7): 1525-1536. 10.1139/z90-226.View ArticleGoogle Scholar
- Lacalli TC: Structure and organization of the nervous system in the actinotroch larva of Phoronis vancouverensis. Phil Trans Roy Soc L. 1990, 327: 655-685. 10.1098/rstb.1990.0104.View ArticleGoogle Scholar
- Santagata S: Structure and metamorphic remodeling of the larval nervous system and musculature of Phoronis pallida (Phoronida). Evol Dev. 2002, 4: 28-42. 10.1046/j.1525-142x.2002.01055.x.PubMedView ArticleGoogle Scholar
- Santagata S: Evolutionary and structural diversification of the larval nervous system among marine bryozoans. Biol Bull. 2008, 215: 3-23. 10.2307/25470679.PubMedView ArticleGoogle Scholar
- Zimmer RL: PhD Thesis. Reproductive biology and development of Phoronida. 1964, Ann Arbor: University Microfilm, 416-Google Scholar
- Stricker S, Reed C, Zimmer R: The cyphonautes larva of the marine bryozoan Membranipora membranacea. II. Internal sac, musculature, and pyriform organ. Can J Zool. 1988, 66: 384-398. 10.1139/z88-055.View ArticleGoogle Scholar
- Temereva EN: The digestive tract of actinotroch larvae (Lophotrochozoa, Phoronida): anatomy, ultrastructure, innervations, and some observations of metamorphosis. Can J Zool. 2010, 88 (2): 1149-1168.View ArticleGoogle Scholar
- Fernández I, Pardos F, Benito J, Roldan C: Ultrastructural observation on the phoronid nervous system. J Morphol. 1996, 230: 265-281. 10.1002/(SICI)1097-4687(199612)230:3<265::AID-JMOR2>3.0.CO;2-D.View ArticleGoogle Scholar
- Malosio ML, Giordano T, Laslop A, Meldolesi J: Dense-core granules: a specific hallmark of the neuronal/neurosecretory cell phenotype. J Cell Sci. 2004, 117: 743-749. 10.1242/jcs.00934.PubMedView ArticleGoogle Scholar
- Santagata S: Evaluating neurophylogenetic patterns in the larval nervous systems of brachiopods and their evolutionary significance to other bilaterian phyla. J Morphol. 2011, 272: 1153-1169. 10.1002/jmor.10975.PubMedView ArticleGoogle Scholar
- Altenburger A, Martinez P, Wanninger A: Homeobox gene expression in Brachiopoda: the role of Not and Cdx in bodyplan patterning, neurogenesis, and germ layer specification. Gene Expres Patt. 2011, 11: 427-436. 10.1016/j.gep.2011.07.001.View ArticleGoogle Scholar
- Freeman G, Lundelius JW: The transition from planktotrophy to lecithotrophy in larvae of Lower Palaeozoic Rhynchonelliform brachiopods. Lethaia. 2005, 38: 219-254. 10.1080/00241160510013330.View ArticleGoogle Scholar
- Nezlin LP, Yushin VV: Structure of the nervous system in the tornaria larva of Balanoglossus proterogonius (Hemichordata: Enteropneusta) and its phylogenetic implications. Zoomorphol. 2004, 123: 1-13. 10.1007/s00435-003-0086-z.View ArticleGoogle Scholar
- Byrne M, Nakajima Y, Chee FC, Burke RD: Apical organs in echinoderm larvae: insights into larval evolution in the Ambulacraria. Evol Dev. 2007, 9: 432-445. 10.1111/j.1525-142X.2007.00189.x.PubMedView ArticleGoogle Scholar
- Nielsen C, Hay-Schmidt A: Development of the Enteropneust Ptychodera flava: ciliary bands and nervous system. J Morphol. 2007, 268: 551-570. 10.1002/jmor.10533.PubMedView ArticleGoogle Scholar
- Burke RD: Deuterostomy neuroanatome and the body plan paradox. Evol Dev. 2011, 13 (1): 110-115. 10.1111/j.1525-142X.2010.00460.x.PubMedView ArticleGoogle Scholar
- Voronezhskaya EE, Tyurin SA, Nezlin LP: Neuronal development in larval chiton Ischnochiton hakodadensis(Mollusca: Polyplacophora). J Comp Neurol. 2002, 444: 25-38. 10.1002/cne.10130.PubMedView ArticleGoogle Scholar
- McDougall C, Chen W-C, Shimeld SM, Ferrier D: The development of the larval nervous system, musculature and ciliary bands of Pomatoceros lamarckii (Annelida): heterochrony in polychaetes. Front Zool. 2006, 3 (16): doi:10.1186/1742-9994-3-16Google Scholar
- Nezlin LP: The golden age of comparative morphology: laser scanning microscopy and neurogenesis in trochophore animals. Russ J Dev Biol. 2010, 41 (6): 381-390. 10.1134/S1062360410060056.View ArticleGoogle Scholar
- Voronezhskaya EE, Tsitrin EB, Nezlin LP: Neuronal development in larval Polychaete Phyllodoce maculate (Phyllodocidae). J Comp Neurol. 2003, 455: 299-309. 10.1002/cne.10488.PubMedView ArticleGoogle Scholar
- Voronezhskaya EE, Nezlin LP, Odintsova NA: Neuronal development in larval mussel Mytilus trossulus (Mollusca: Bivalvia). Zoomorphol. 2008, 127: 97-110. 10.1007/s00435-007-0055-z.View ArticleGoogle Scholar
- Chernyshev AV, Magarlamov TY: The first data on the nervous system of hoplonemertean larvae (Nemertea, Hoplonemertea). Dokl Biol Sci. 2010, 430 (1): 48-50. 10.1134/S0012496610010163.PubMedView ArticleGoogle Scholar
- Chernyshev AV, Astakhova AA, Dautov SS, Yushin VV: Morphology of the apical organ and adjacent epithelium of pilidium prorecurvatum, a pelagic larva of unknown heteronemertean (Nemertea). Russ J Mar Biol. 2013, 39 (2): 106-114.View ArticleGoogle Scholar
- Maslakova SA: Development to metamorphosis of the nemertean pilidium larva. Front Zool. 2010, 7: 30-10.1186/1742-9994-7-30.PubMedPubMed CentralView ArticleGoogle Scholar
- Nakano H, Murabe N, Amemiya S, Nakajima Y: Nervous system development in the sea cucumber Stichopus japonicus. Dev Biol. 2006, 292: 205-212. 10.1016/j.ydbio.2005.12.038.PubMedView ArticleGoogle Scholar
- Sly BJ, Snoke MS, Raff RA: Who came first - larvae or adults? Origins of bilaterian metazoan larvae. Int J Dev Biol. 2003, 47: 623-632.PubMedGoogle Scholar
- Nielsen C: Life cycle evolution: was the eumetazoan ancestor a holopelagic, planktotrophic gastraea?. BMC Evol Biol. 2013, 13: 171-10.1186/1471-2148-13-171.PubMedPubMed CentralView ArticleGoogle Scholar
- Raff RA: Origins of the other metazoan body plans: the evolution of larval forms. Phil Trans R Soc B. 2008, 363 (1496): 1473-1479. 10.1098/rstb.2007.2237.PubMedPubMed CentralView ArticleGoogle Scholar
- Valentine JW, Collins AG: The significance of moulting in Ecdysozoan evolution. Evol Dev. 2000, 2 (3): 152-156. 10.1046/j.1525-142x.2000.00043.x.PubMedView ArticleGoogle Scholar
- Gharbiah M, Nakamoto A, Nagy L: Analysis of ciliary band formation in the mollusc Ilyanassa obsoleta. Dev Genes Evol. 2013, 223: 225-235. 10.1007/s00427-013-0440-1.PubMedView ArticleGoogle Scholar
- Butler AB: Sensory systems and brain evolution across the bilateria: Commonalities and constraints. Sensory Processing in Aquatic Environments. Edited by: Collin SP, Marshall NJ. 2003, NY: Springer-Verlag, 375-388.View ArticleGoogle Scholar
- Bailly X, Reichert H, Hartenstein V: The urbilaterian brain revisited: novel insights into old questions from new flatworm clades. Dev Genes Evol. 2013, Doi:10.1007/s00427-012-0423-7Google Scholar
- Holland LZ, Carvalho JE, Escriva H, Laudet V, Schubert M, Shimeld SM, Yu J-K: Evolution of bilaterian central nervous systems: a single origin?. EvoDevo. 2013, 4: 27-10.1186/2041-9139-4-27.http://www.evodevojournal.com/content/4/1/27,PubMedPubMed CentralView ArticleGoogle Scholar
- Peterson KJ, Eernisse DJ: Animal phylogeny and the ancestry of bilaterians: inferences from morphology and 18S rDNA gene sequences. Evol Dev. 2001, 3: 170-205. 10.1046/j.1525-142x.2001.003003170.x.PubMedView ArticleGoogle Scholar
- Temereva EN, Malakhov VV: The evidence of metamery in adult brachiopods and phoronids. Invert Zool. 2011, 8 (2): 91-112.Google Scholar
- Temereva EN, Neretina TV: A distinct phoronid larva: morphological and molecular evidence. Inver Syst. 2013, 27 (6): 622-633.Google Scholar
- Richter S, Loesel R, Purschke G, Schmidt-Rhaesa A, Scholtz G, Stach T, Vogt L, Wanninger A, Brenneis G, Doring C: Invertebrate neurophylogeny: suggested terms and definitions for a neuroanatomical glossary. Front Zool. 2010, 7 (1): 29-10.1186/1742-9994-7-29.PubMedPubMed CentralView ArticleGoogle Scholar
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