- Open Access
Homologs of wingless and decapentaplegic display a complex and dynamic expression profile during appendage development in the millipede Glomeris marginata (Myriapoda: Diplopoda)
© Prpic; licensee BioMed Central Ltd. 2004
- Received: 27 October 2004
- Accepted: 24 November 2004
- Published: 24 November 2004
The Drosophila genes wingless (wg) and decapentaplegic (dpp) comprise the top level of a hierarchical gene cascade involved in proximal-distal (PD) patterning of the legs. It remains unclear, whether this cascade is common to the appendages of all arthropods. Here, wg and dpp are studied in the millipede Glomeris marginata, a representative of the Myriapoda.
Glomeris wg (Gm-wg) is expressed along the ventral side of the appendages compatible with functioning during the patterning of both the PD and dorsal-ventral (DV) axes. Gm-wg may also be involved in sensory organ formation in the gnathal appendages by inducing the expression of Distal-less (Dll) and H15 in the organ primordia. Expression of Glomeris dpp (Gm-dpp) is found at the tip of the trunk legs as well as weakly along the dorsal side of the legs in early stages. Taking data from other arthropods into account, these results may be interpreted in favor of a conserved mode of WG/DPP signaling. Apart from the main PD axis, many arthropod appendages have additional branches (e.g. endites). It is debated whether these extra branches develop their PD axis via the same mechanism as the main PD axis, or whether branch-specific mechanisms exist. Gene expression in possible endite homologs in Glomeris argues for the latter alternative.
All available data argue in favor of a conserved role of WG/DPP morphogen gradients in guiding the development of the main PD axis. Additional branches in multibranched (multiramous) appendage types apparently do not utilize the WG/DPP signaling system for their PD development. This further supports recent work on crustaceans and insects, that lead to similar conclusions.
- Ventral Side
- Sensory Organ
- Dorsal Side
- Morphogen Gradient
- Appendage Development
The genes wingless (wg) and decapentaplegic (dpp) are important factors for the normal development of the Drosophila legs. Both genes encode secreted morphogens that generate combinatorial gradients across the developing imaginal leg discs (e.g. ). These gradients form the top level in a PD axis patterning cascade and they control expression of the genes at the next level of the cascade, the leg-gap genes (e.g. Distal-less (Dll), dachshund (dac)) (e.g. [2, 3]). Thus, wg and dpp are key factors involved in the early events of PD axis formation.
In recent years several comparative studies in other arthropod species have suggested that the action of the leg-gap genes in PD patterning is evolutionarily conserved [4–9]. Tus, the question arose as to whether the regulation of the leg-gap genes by the WG/DPP morphogen gradient is also conserved. The currently available data provide no clear answer. Initially, the expression patterns did not support the conservation of this top level of the PD axis patterning cascade [9, 10]. Other authors, however, have argued in favor of a conservation of WG/DPP morphogen signaling in PD axis formation . Furthermore, many arthropods have appendages with more than one PD axis. It is currently debated whether these multiple axes are all patterned by a cascade involving wg and dpp at the top level, or whether the different branches are patterned through branch-specific mechanisms.
The comparative analyses of wg and dpp expression during appendage formation to date mainly focus on insects (e.g. Tribolium, Gryllus, Schistocerca, Athalia [9–12]). Only a few representatives of the crustaceans and chelicerates have been studied from other arthropod classes [6, 13]. Here, I report on results concerning wg and dpp expression in the appendages of a representative of the fourth extant arthropod class, the myriapod Glomeris marginata. The wg gene of Glomeris is expressed on the ventral side of the appendages compatible with a conserved role in PD axis development. Additionally, Glomeris wg may induce expression of the genes Dll and H15 in the sensory organs of the mouthparts. The results with the Glomeris dpp gene are more ambiguous. Although the data can be interpreted in favor of a conservation of the WG/DPP morphogen gradients, clearly more work on the subject is necessary to clarify the evolution of PD axis patterning in arthropod appendages. In particular, it will be necessary to elucidate the mechanisms through which the additional PD axes in multibranched appendages are patterned.
Cloning of Gm-dpp cDNA fragments
Expression of Gm-dpp during embryogenesis
In younger stages, a specific staining is seen in the forming appendage buds and along the external, i.e. dorsal, rim of the neuroectoderm (Fig. 2A; arrow). It is known that the neuroectoderm of each hemisegment is divided into a dorsal, medial and ventral portion . Judging from its expression, it is possible that Gm-dpp has a role in the development of the dorsal portion of the neuroectoderm. A role in the developing ventral portion is also possible, since Gm-dpp is transiently expressed along the ventral midline (Fig. 2B; arrows). A further expression domain in the central nervous system is seen in the area of the developing optic centers of the brain (Fig. 2C,2D,2E; arrows).
Starting with stage 4, Gm-dpp is expressed along the external rim of the germband in tissue that will later form the heart (Fig. 2D,2E; arrowheads). Later on, segmentally repeated patches of weak Gm-dpp expression appear on the dorsal side of the embryos (Fig. 2F; arrowheads). These patches are presumably also correlated with the developing heart of the embryos. Finally, expression of Gm-dpp is found in the stomodaeum, and very weakly in the proctodaeum.
Expression profile of Gm-dpp during appendage development
The appendages buds show weak expression of Gm-dpp at the very beginning of their formation (Fig. 2A,2B). Later on, different appendages display appendage-specific expression patterns. In the trunk legs, the strongest expression is seen at the leg tips. In early developmental stages the expression fills almost the entire tip, and the border against the ventral portion of the legs (which is devoid of expression) is rather distinct (Fig. 3A). There is also expression of Gm-dpp along the dorsal side of the trunk legs, but this is visibly weaker than the expression in the leg tips. The expression at the leg tips is clearly confined to the dorsal side of the tip in legs of stage 5 embryos (Fig. 3E), while the expression along the dorsal side persists, but becomes weaker and diffuse. Finally, expression of Gm-dpp in the legs vanishes almost completely at stage 6 (Fig. 3I). The dorsal expression is virtually undetectable, and only a few cells express Gm-dpp at the tip.
In the maxilla there are two expression domains of Gm-dpp, a dorsal and a ventral one (Fig. 3B). The ventral domain is located on the internal side at the base of the maxilla. This domain slowly vanishes during development (Fig. 3F) and finally disappears around stage 6 (Fig. 3J). The dorsal expression domain runs along the dorsal edge of the base of the maxilla (Fig. 3B,3F). This domain also gradually disappears during development, and at stage 6.1 only a faint dorsal expression is detectable (Fig. 3J).
In the mandible, a dorsal expression domain that runs along the entire dorsal rim of the appendage is visible (Fig. 3C). Later on, however, this expression is restricted to the basal portion of the mandible and has a distinct border against the external lobe (Fig. 3G). Additional expression domains are detectable at later stages within the external lobe (Fig. 3K; asterisk) in addition to the internal side of the internal lobe (Fig. 3G,3K; arrow).
In the antenna, Gm-dpp is expressed in the dorsal half of the appendage with a distinct border against the non-expressing ventral half (Fig. 3D). In addition, a patch of weaker Gm-dpp expression is located on the ventral side of the antenna (Fig. 3D; square). Another patch of Gm-dpp expression is visible at the transition between the antennal base and the neuroectoderm of the antennal neuromere (Fig. 3D; asterisk). The latter two patches of expression disappear during the further course of development. By stage 5 the ventral spot has disappeared completely, and the patch at the antennal base is virtually gone as well (Fig. 3H). Similar to the other appendages at stage 6.1, the level of Gm-dpp expression has also significantly decreased, though one can discern three specific expression domains at this stage. There are two groups of cells (at the tip and at the base of the antenna) weakly expressing Gm-dpp (Fig. 3L; arrow and asterisk, respectively), and a ring of cells at the distal third of the antenna, where Gm-dpp expression is even weaker (Fig. 3L; square).
Expression profile of Gm-wg during appendage development
In the trunk legs expression of Gm-wg is restricted to the ventral side during the further course of development (Fig. 4E,4I,4M,4Q). The expression is contiguous from the base of the legs to the tips, but the level of expression is somewhat heterogeneous. The strongest expression is seen near the base and in the distal part of the legs, while expression is visibly weaker between these parts. A similar phenomenon is present in the antenna (Fig. 4H,4L,4P,4T), where expression is restricted to the ventral side of the antenna and the level of expression at the distal end is much stronger than in more proximal parts. However, unlike the pattern in the legs, the intensity of expression at the base of the antenna is not increased.
The maxilla displays a rather dynamic expression profile of Gm-wg. Beginning at stage 4 the gene is expressed along the ventral edge of the maxilla (Fig. 4F). Three domains can be distinguished that are not completely separated. The innermost domain is more diffuse than the other two domains and at stage 5 separates into two separate patches of expression (Fig. 4J,4N,4R; two-headed arrow). The two other domains remain separate during the development of the maxillary appendage and are reminiscent of the expression pattern of Gm-Dll (see below).
In the mandible, a similar fragmentation of the initial mostly homogeneous expression pattern takes place. In the external lobe the expression is strong throughout and is separated from the expression domain in the internal lobe by an area of very weak expression (Fig. 4G,4K,4O,4S). The expression domain in the internal lobe splits (Fig. 4K), then retracts from the tip of the lobe (Fig. 4O) and decreases in expression strength (Fig. 4S).
Expression of Gm-wg and Gm-Dll in the gnathal sensory organs
In addition, a complex and dynamic pattern of Gm-Dll has been described in the mandible . In contrast to the maxilla, the patterns of Gm-wg and Gm-Dll appear to overlap completely in the mandible. In preparations of mandibles labeled with a cocktail of probes against both genes no significant difference to the pattern of Gm-wg alone is observed (Fig. 5B,5D), indicating that the Gm-Dll pattern is entirely included in the Gm-wg pattern.
Establishment of the primary PD axis
In Drosophila dpp is expressed in a narrow dorsal sector in the leg imaginal discs, whereas wg is expressed in a similar sector on the ventral side (e.g. ). Together these two genes generate morphogen gradients in the developing leg imaginal discs. These gradients are utilized by several genes to guide the development of the PD axis of the leg imaginal discs. Evolutionary developmental studies have shown that the expression of wg homologs along the ventral side of the appendages is highly conserved in the arthropods (e.g. [6, 9, 10, 13]). In contrast, dpp expression differs from the expression pattern found in Drosophila in all arthropods studied thus far (e.g. [6, 9–12, 18, 19]). At early stages expression of arthropod dpp homologs is restricted to the leg tip, while at later stages expression rings of unclear significance appear in some species. Despite these differences in expression, it has been argued that the combined action of the WG and DPP morphogen gradients is conserved, and that the differences in expression of dpp are correlated with the differences in the mode of leg development between Drosophila (via imaginal discs) and most other arthropods (normal leg outgrowth) .
The data from Glomeris presented here may be interpreted in favor of this hypothesis. The Gm-wg gene is expressed along the ventral side in the legs and Gm-dpp is expressed most strongly in the leg tips. Taking these expression loci as the sources of Gm-WG and Gm-DPP protein, the resulting hypothetical protein gradients would facilitate PD patterning events similar to the ones in the Drosophila leg discs (see also Fig. 11 in ). However, Gm-dpp is weakly expressed along the dorsal leg side. This is similar to the Drosophila situation, but is contrary to the predictions of the above hypothesis since Glomeris does not develop the legs via flat imaginal discs and therefore should show a dpp expression pattern typical of directly developing legs rather than a pattern similar to Drosophila. The fact that Gm-dpp is also weakly expressed along the dorsal side of the legs may be explained by several possibilities. It may be argued that the dorsal expression is so weak that it has no significant influence on the shape of the Gm-DPP protein gradient, which would therefore mainly be dependent on the morphogen source at the tip. It is also possible that the dorsal expression is unrelated to PD axis formation and instead functions during DV axis formation (see below).
In any case, the picture emerging from the available data on dpp expression in arthropods is that the dorsal sector in Drosophila seems to be an exception rather than the rule. The hypothesis proposed by Prpic et al.  attempts to explain this by the differences in leg architecture between Drosophila and most other arthropod species. However, according to their hypothesis, the presence of combinatorial protein gradients is conserved. It should be pointed out in this context that the existence of a DPP gradient (or a WG gradient for that matter) has yet to be demonstrated in an arthropod other than Drosophila. Thus, although the expression data may be interpreted as the PD axis patterning using WG/DPP signaling being conserved among arthropods, it is obvious that comparative expression analyses alone cannot answer the question satisfactorily. It must now be considered whether experiments capable of demonstrating WG/DPP signaling during leg development in arthropods other than Drosophila may be conceived.
Establishment of secondary PD axes
Aside from the primary PD axis, many arthropods have limbs with additional branches (rami). It has been proposed that these additional rami are patterned in the same way as the main branch, simply by duplications of the WG/DPP signaling system . Recent results from the study of insect mouthparts argue against this notion . The insect labium and maxilla have ventral branches (endites) that apparently do not utilize a combinatorial WG/DPP gradient system to guide their outgrowth. A similar conclusion has been reached by a study of the development of crustacean multibranched appendages .
The presence of endites in the mouthparts of myriapods is unclear, mainly because of the modified morphology of the adult gnathalia. Certain elements of the centipede mandible and first maxilla are probably derived from endites (e.g. [21, 22]) and there are attempts to assign parts of the diplopod mandible as homologous to crustacean or insect endites (e.g. [21, 23]). Indeed, the embryonic mandible and maxilla in Glomeris develop ventral lobes that are very reminiscent of the endite lobes of the embryonic mouthparts in insects. The exclusive ventral origin of these lobes is further corroborated by the lack of expression of the dorsal marker optomotor-blind . Furthermore, the Glomeris lobes possess Dll-positive sensory organs, which is typical of arthropod endites [7, 24–26]. Thus, although the interpretation of the millipede mouthparts is disputed (see e.g. ), these ventral lobes are likely homologous to the endites present in insect mouthparts.
In summary, none of the maxillary and mandibulary lobes in Glomeris appear to utilize conventional WG/DPP signaling to organize PD growth. Similar results have been obtained recently for the endites in the grasshopper Schistocerca and the beetle Tribolium . In Schistocerca at least one endite (the galea) grows without dpp expression (Fig. 6D), and in Tribolium both maxillary endites lack detectable dpp expression . This indicates that the development of the PD axis of the endites does not generally require the WG/DPP morphogen system.
Relation of wingless and dpp expression to DV axis formation
A second role of wg and dpp in Drosophila is the activation of some factors involved in DV axis formation in the legs [28, 29]. wg, being expressed along the ventral side, is an instructor of ventral fate, whereas dpp is expressed on the dorsal side and establishes dorsal fates. The primary factors controlled by wg and dpp are H15 on the ventral side and omb on the dorsal side. These factors have been recently studied in Glomeris and in a spider (Cupiennius salei) [6, 17]. The expression patterns suggest that the role of omb as dorsal instructor is evolutionarily conserved, but H15 does not seem to be a general ventralizing factor in all arthropods. Thus, the dorsal, but not the ventral developmental mechanisms seem to be conserved. It is interesting that the expression data of wg and dpp suggest that the opposite is true. The wg expression on the ventral side is highly conserved among the arthropods, but the dpp patterns differ between species and in most part expression is not localized to the entire dorsal side. This paradox clearly demonstrates the limited understanding of the evolution of DV axis formation in arthropod appendages.
Patterning of appendicular sensory organs
The maxilla of Glomeris has several sensory organs. Recent studies have identified the genes Dll, dac and H15, which show a restricted expression pattern in the primordia of the maxillary sensory organs [7, 17]. Two of these genes, Dll and H15, are known from Drosophila to be activated upon signaling through the wingless pathway [29, 30]. It is interesting to note that expression of Gm-wg surrounds the sensory primordia in the Glomeris maxilla. It may therefore be the case that cells expressing Gm-wg in the surrounding of the primordia signal to their neighbors within the primordia and stimulate them to activate Gm-Dll and Gm-H15. Minimally the activation of Dll appears to be a general feature of appendicular sensory organs in arthropods since Dll expression has been observed in appendicular sensory organs in chelicerates, crustaceans, myriapods and insects (e.g. [7, 24, 25, 31]). Moreover, data from Drosophila suggest that Dll expression is critically required for sensory organ formation, as mutants lacking Dll fail to develop Keilin's organs (the sensory structures of the embryonic leg anlagen) [32, 33].
The expression of Gm-wg and Gm-dpp during appendage development indicates a role for both genes in guiding this process. Involvement of wg and dpp in appendage development appears to be conserved among all extant arthropod classes including myriapods. The data from Glomeris and other arthropods suggest that the WG/DPP morphogen signaling system as it is known from Drosophila leg discs is present in all arthropods. However, this morphogen system apparently functions in only the main branch of the appendages, the so-called telopodite . Limb types with additional branches (e.g. endites) obviously use additional, yet unidentified mechanisms to organize proximal-distal growth of the extra branches. Gene expression in potential endite homologs present in Glomeris mouthparts supports this notion. Aside from the role in PD axis formation, the expression profile of Gm-wg suggests an additional role for this gene in patterning appendicular sensory organs.
Animals were collected during Spring 2003 in beech forests in the vicinity of Cologne, Germany and near Kranenburg, Germany. They have been treated as described before [6, 14]. The animals were released after the end of the breeding season (Summer '03).
The cloning assays were based on cDNA transcribed from polyA-RNA extracted from selected Glomeris embryos of all developmental stages up to stage 6.1 (see [14, 35] for a description of embryonic stages) and were performed in duplicate. For the amplification of dpp-like gene fragments, the primers dpp-fw-1 (GAY GTN GGN TGG GAY GAY TGG) and dpp-bw-1 (CKR CAN CCR CAN CCN CAN AC) were used in the initial PCR, and the primers dpp-fw-2 (GGN TAY GAY GCN TAY TAY TG) and dpp-bw-1 were used in the nested PCR. Additional sequence information was gained by RACE PCR. No full-length fragment could be obtained and several artificial clones were encountered, probably representing chimeric products resulting from jumping PCR between different TGF-beta-like cDNAs. Using species specific primers, artificial and genuine fragments were identified. A confirmed genuine fragment of almost 360 bp was isolated and cloned. This fragment was used for sequence analysis and probe synthesis. The isolation of Gm-wg has been previously reported . The GenBank accession numbers are as follows: Gm-wg (AJ616907); Gm-dpp (AJ843875).
Alignments and sequence analysis
Pairwise alignments of aminoacid sequences were performed by searching GenBank  using the Gapped BLAST program . The alignments were calculated based on the BLOSUM 62 matrix  (gap costs: 11 for opening, 1 for extension). Multiple sequence alignments were calculated based on the GONNET matrix  (gap costs: 10 for opening, 0.2 for extension) implemented in CLUSTAL_X . The resulting alignments were subjected to maximum likelihood analysis using the Quartet Puzzling method  as implemented in PAUP* 4.0b10 .
In situ hybridizations, specimen preparation and microscopy
In situ hybridization with digoxigenin-labeled RNA probes has been performed as previously described . Whole-mount embryos were photographed in PBST under a Leica dissection microscope. Appendages were dissected with fine insect needles and photographed in 50% glycerol under a Zeiss Axioplan microscope. All images were corrected for color values, brightness and contrast using Adobe Photoshop 5.5 for Apple Macintosh. The image processing software has also been used to enhance image backgrounds by retouching dirt or yolk remains, and to group together single pictures into multipanel figures.
I thank Diethard Tautz for his encouragement and advice during all phases of my work. The clone of Gm-wg has been a gift from Ralf Janssen. The Glomeris RACE template has been a gift from Hilary Dove. I also thank Wim Damen for comments on the manuscript. I thank John Baines for his help with the English language. This work has been funded by a grant from the Deutsche Forschungsgemeinschaft (grant number TA99/19-2).
- Lecuit T, Cohen SM: Proximal-distal axis formation in the Drosophila leg. Nature. 1997, 388: 139-145. 10.1038/40563.View ArticlePubMedGoogle Scholar
- Rauskolb C, Irvine KD: Notch-mediated segmentation and growth control of the Drosophila leg. Dev Biol. 1999, 210: 339-350. 10.1006/dbio.1999.9273.View ArticlePubMedGoogle Scholar
- Rauskolb C: The establishment of segmentation in the Drosophila leg. Development. 2001, 128: 4511-4521.PubMedGoogle Scholar
- Abzhanov A, Kaufman TC: Homologs of Drosophila appendage genes in the patterning of arthropod limbs. Dev Biol. 2000, 227: 673-689. 10.1006/dbio.2000.9904.View ArticlePubMedGoogle Scholar
- Angelini DR, Kaufman TC: Functional analyses in the hemipteran Oncopeltus fasciatus reveal conserved and derived aspects of appendage patterning in insects. Dev Biol. 2004, 271: 306-321. 10.1016/j.ydbio.2004.04.005.View ArticlePubMedGoogle Scholar
- Prpic NM, Janssen R, Wigand B, Klingler M, Damen WGM: Gene expression in spider appendages reveals reversal of exd/hth spatial specificity, altered leg gap gene dynamics, and suggests divergent distal morphogen signaling. Dev Biol. 2003, 264: 119-140. 10.1016/j.ydbio.2003.08.002.View ArticlePubMedGoogle Scholar
- Prpic NM, Tautz D: The expression of the proximodistal axis patterning genes Distal-less and dachshund in the appendages of Glomeris marginata (Myriapoda: Diplopoda) suggests a special role of these genes in patterning the head appendages. Dev Biol. 2003, 260: 97-112. 10.1016/S0012-1606(03)00217-3.View ArticlePubMedGoogle Scholar
- Inoue Y, Mito T, Miyawaki K, Matsushima K, Shinmyo Y, Heanue TA, Mardon G, Ohuchi H, Noji S: Correlation of expression patterns of homothorax, dachshund, and Distal-less with the proximodistal segmentation of the cricket leg bud. Mech Dev. 2002, 113: 141-148. 10.1016/S0925-4773(02)00017-5.View ArticlePubMedGoogle Scholar
- Jockusch EL, Nulsen C, Newfeld SJ, Nagy LM: Leg development in flies versus grasshoppers: differences in dpp expression do not lead to differences in the expression of downstream components of the leg patterning pathway. Development. 2000, 127: 1617-1626.PubMedGoogle Scholar
- Niwa N, Inoue Y, Nozawa A, Saito M, Misumi Y, Ohuchi H, Yoshioka H, Noji S: Correlation of diversity of leg morphology in Gryllus bimaculatus (cricket) with divergence in dpp expression pattern during leg development. Development. 2000, 127: 4373-4381.PubMedGoogle Scholar
- Jockusch EL, Williams TA, Nagy LM: The evolution of patterning of serially homologous appendages in insects. Dev Genes Evol. 2004, 214: 324-338. 10.1007/s00427-004-0412-6.View ArticlePubMedGoogle Scholar
- Yamamoto DS, Sumitani M, Tojo K, Lee JM, Hatakeyama M: Cloning of a decapentaplegic orthologue from the sawfly, Athalia rosae (Hymenoptera), and its expression in the embryonic appendages. Dev Genes Evol. 2004, 214: 128-133. 10.1007/s00427-004-0387-3.View ArticlePubMedGoogle Scholar
- Nulsen C, Nagy LM: The role of wingless in the development of multibranched crustacean limbs. Dev Genes Evol. 1999, 209: 340-348. 10.1007/s004270050262.View ArticlePubMedGoogle Scholar
- Janssen R, Prpic NM, Damen WGM: Gene expression suggests decoupled dorsal and ventral segmentation in the millipede Glomeris marginata (Myriapoda: Diplopoda). Dev Biol. 2004, 268: 89-104. 10.1016/j.ydbio.2003.12.021.View ArticlePubMedGoogle Scholar
- Dove H, Stollewerk A: Comparative analysis of neurogenesis in the myriapod Glomeris marginata (Diplopoda) suggests more similarities to chelicerates than to insects. Development. 2003, 130: 2161-2171. 10.1242/dev.00442.View ArticlePubMedGoogle Scholar
- Dove H: Neurogenesis in the millipede Glomeris marginata (Myriapoda: Diplopoda). PhD thesis. 2003, Universität zu Köln, Mathematisch-Naturwissenschaftliche FakultätGoogle Scholar
- Prpic NM, Janssen R, Damen WGM, Tautz D: Evolution of dorsal-ventral axis formation in arthropod appendages: H15 and optomotor-blind/bifid-type T-box genes in the millipede Glomeris marginata (Myriapoda: Diplopoda). Evol Dev.Google Scholar
- Sanchez-Salazar J, Pletcher MT, Bennett RL, Brown SJ, Dandamudi TJ, Denell RE, Doctor JS: The Tribolium decapentaplegic gene is similar in sequence, structure, and expression to the Drosophila dpp gene. Dev Genes Evol. 1996, 206: 237-246. 10.1007/s004270050049.View ArticlePubMedGoogle Scholar
- Akiyama-Oda Y, Oda H: Early patterning of the spider embryo: a cluster of mesenchymal cells at the cumulus produces Dpp signals received by germ disc epithelial cells. Development. 2003, 130: 1735-1747. 10.1242/dev.00390.View ArticlePubMedGoogle Scholar
- Panganiban G, Sebring A, Nagy L, Carroll S: The development of crustacean limbs and the evolution of arthropods. Science. 1995, 270: 1363-1366.View ArticlePubMedGoogle Scholar
- Snodgrass RE: Principles of Insect Morphology. 1935, New York: McGraw-Hill Book CompanyGoogle Scholar
- Shukla GS: Studies on Scolopendra morsitans Linn. Part I: External features and skeleton. Zool Anz. 1963, 170: 131-149.Google Scholar
- Richter S, Edgecombe GD, Wilson GDF: The lacinia mobilis and similar structures – a valuabloe character in arthropod phylogenetics?. Zool Anz. 2002, 241: 339-361.View ArticleGoogle Scholar
- Mittmann B, Scholtz G: Distal-less expression in embryos of Limulus polyphemus (Chelicerata, Xiphosura) and Lepisma saccharina (Insecta, Zygentoma) suggests a role in the development of mechanoreceptors, chemoreceptors, and the CNS. Dev Genes Evol. 2001, 211: 232-243. 10.1007/s004270100150.View ArticlePubMedGoogle Scholar
- Williams TA, Nulsen C, Nagy LM: A complex role for Distal-less in crustacean appendage development. Dev Biol. 2002, 241: 302-312. 10.1006/dbio.2001.0497.View ArticlePubMedGoogle Scholar
- Prpic NM, Damen WGM: Expression patterns of leg genes in the mouthparts of the spider Cupiennius salei (Chelicerata: Arachnida). Dev Genes Evol. 2004, 214: 296-302. 10.1007/s00427-004-0393-5.View ArticlePubMedGoogle Scholar
- Hilken G, Kraus O: Struktur und Homologie der Komponenten des Gnathochilarium der Chilognatha (Tracheata, Diplopoda). Verh naturwiss Ver Hamburg NF. 1994, 34: 33-50.Google Scholar
- Jiang J, Struhl G: Complementary and mutually exclusive activities of Decapentaplegic and Wingless organize axial patterning during Drosophila leg development. Cell. 1996, 86: 401-409. 10.1016/S0092-8674(00)80113-0.View ArticlePubMedGoogle Scholar
- Brook WJ, Cohen SM: Antagonistic interactions between Wingless and Decapentaplegic responsible for dorsal-ventral pattern in the Drosophila leg. Science. 1996, 273: 1373-1377.View ArticlePubMedGoogle Scholar
- Cohen B, Simcox AA, Cohen SM: Allocation of the thoracic imaginal primordia in the Drosophila embryo. Development. 1993, 117: 597-608.PubMedGoogle Scholar
- Williams TA: Distalless expression in crustaceans and the patterning of branched limbs. Dev Genes Evol. 1998, 207: 427-434. 10.1007/s004270050133.View ArticlePubMedGoogle Scholar
- Sunkel CE, Whittle JRS: Brista: a gene involved in the specification and differentiation of distal cephalic and thoracic structures in Drosophila melanogaster. Roux Arch Dev Biol. 1987, 196: 124-132. 10.1007/BF00402034.View ArticleGoogle Scholar
- Cohen SM, Jürgens G: Proximal-distal pattern formation in Drosophila: cell autonomous requirement for Distal-less gene activity in limb development. Embo J. 1989, 8: 2045-2055.PubMed CentralPubMedGoogle Scholar
- Boxshall GA: The evolution of arthropod limbs. Biol Rev. 2004, 79: 253-300. 10.1017/S1464793103006274.View ArticlePubMedGoogle Scholar
- Dohle W: Die Embryonalentwicklung von Glomeris marginata (Villers) im Vergleich zur Entwicklung anderer Diplopoden. Zool Jb Anat. 1964, 81: 241-310.Google Scholar
- Benson DA, Karsch-Mizrachi I, Lipman DJ, Ostell J, Wheeler DL: GenBank: update. Nucleic Acids Res. 2004, Suppl 1: D23-D26. 10.1093/nar/gkh045.View ArticleGoogle Scholar
- Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ: Gapped BLAST and PsiBLAST: a new generation of protein database search programs. Nucleic Acids Res. 1997, 25: 3389-3402. 10.1093/nar/25.17.3389.PubMed CentralView ArticlePubMedGoogle Scholar
- Henikoff S, Henikoff JG: Amino acid substitution matrices from protein blocks. Proc Natl Acad Sci USA. 1992, 89: 10915-10919.PubMed CentralView ArticlePubMedGoogle Scholar
- Gonnet GH, Cohen MA, Brenner SA: Exhaustive matching of the entire protein sequence database. Science. 1992, 256: 1443-1445.View ArticlePubMedGoogle Scholar
- Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG: The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 1997, 25: 4876-4882. 10.1093/nar/25.24.4876.PubMed CentralView ArticlePubMedGoogle Scholar
- Strimmer K, von Haeseler A: Quartet puzzling: a quartet maximum likelihood method for reconstructing tree topologies. Mol Biol Evol. 1996, 13: 964-969.View ArticleGoogle Scholar
- Swofford DL: PAUP*. Phylogenetic Analysis Using Parsimony (*and Other Methods). Version 4. 2002, Sinauer Associates, Sunderland MassachusettsGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.