Fig. 7

FMRF-LIR in the nervous system of Cateria styx. Anterior is up in all panels. A Schematic representation, ventral view. FMRF-LIR is overlaid upon a background representing α-tubulin-LIR; grey shading on the left margin marks the different body regions. B–F Confocal Z-stack projections of specimens co-labeled with FMRFamide (B–F) and DAPI (E). Autofluorescence of the cuticle (grey) was kept as guidance in (B, C, E, F). Colour legend in (C) applies to (B, C, F). B Detail of the brain neuropil and associated somata, ventral view. C Same specimen as in (B), detail of the brain neuropil and associated somata, dorsal view. C’ Zoom into the brain neuropil in (C) showing the associated somata (marked by arrowheads), dorsal view. D Detail of the brain neuropil and associated somata in ventral view. E same image as in (D) including DAPI labelling. F detail of the ventral nerve cord and associated somata (dashed circles) of segments 3-4, ventral view. Arrowheads mark the position of FMR-LIR somata of the brain in (C’–E). Scale bars: 20 μm. Abbreviations: asb, anterior somata of the brain; cne, convergent neurite; i, introvert; n, neck; np, neuropil; psb, posterior somata of the brain; t, trunk; vnc, ventral nerve cord; vncs3/4, ventral nerve cord somata segment 3/4. Numbers refer to segments