Fig. 5

Acetylated α-tub-LIR in the nervous system of Cateria styx. Anterior is up in all panels. A, B Schematic representation in dorsal (A) and ventral (B) view. Grey shading on the left side marks the different body regions. For clarity, innervation of the introvert scalids have been omitted. Only the base of each of the middorsal spines is represented in (A). C–H’ Confocal Z-stack projections of specimens co-labelled with acetylated α-tubulin (C–H’) and DAPI (C–F). Autofluorescence of the cuticle was kept for guidance. Colour legend in (C) applies to all panels. C Segments 1-5 ventral view, introvert everted. Dotted circles mark the position of the ventral nerve cord ganglia. C’ Detail of the ventral nerve cord and ganglia of segments 3-4. D Same specimen as in (C) in dorsal view. E segments 1-4 showing a dorsal view of the brain, located in between segments 2-3, introvert everted. F Same specimen as in (E) with the focal point midway through the brain, showing the positon of the mouth cone. G Segments 9-11, dorsal view. G’ Detail of the innervation of the middorsal spine and sensory spots type 1 of segment 10. H Segments 9-11, ventral view. H’ Detail of the innervation of the ventrolateral spine, sensory spot type5 of segment 10 and terminal spines of segment 11, ventral view, right side. Scale bars: 30 μm (C–F); 10 μm (G–H’). Abbreviations: b, brain; do, dorsal organ; don, dorsal organ neurite; i, introvert; isn, inner oral styles neurite; lnb, longitudinal neurite bundle; mc, mouth cone; mcnr, mouth cone nerve ring; mds, middorsal spine; mts, midterminal spine; np, neuropil; osn, outer oral style neurite; sdn, subdorsal nerve; sn, spine neurite; ssn1/5, sensory spot type1/5 neurite; t, trunk; tn, transverse neurite bundle; tun, tube neurite; tsn, lateral terminal spine neurite; vln, ventrolateral nerve; vnc, ventral nerve cord; vncn, ventral nerve cord neurite bundle. Numbers refer to segments