Fig. 4

Heatmap of 138 statistically significant methylation-sensitive loci (MSL) among seahorse age groups detected using Fisher’s exact test (p < 0.05 after FDR correction). Specimens (rows) and loci (columns) were hierarchically clustered using the unweighted pair group method with arithmetic mean (UPGMA). Loci methylation status (HMM, hemimethylated, HPM, hypermethylated; ICM, internal cytosine methylation; NMT, non-methylated) and the six age groups (1, 5, 10, 20, 30, 40 DAR) are indicated in the right side of the figure. A total of 90 H. reidi individuals were analyzed (n = 15 per group)