Fig. 5

S-phase cell distribution and live observations and in regenerates, cutting level between segments 35 and 36 (L3). a–h′. EdU (pulse-chase) BrdU (pulse) stainings. a–h. Anterior regeneration. e. Confocal a’–h′. Posterior regeneration; arrowheads in b′ and d′ point to EdU chased cells in the stock gut. i–r’. Light microscopy imaging of living specimens. i–r. Anterior regeneration. i′–r’. Posterior regeneration; arrowheads in q’ and r’ point to the region with urinary concretions in the rectal intestine. White dashed lines circumscribe the shape of the animals. Black dashed lines indicate amputation site. Scale bars: 100 μm (c, e, k–n, c′–e’, g’, k′), 200 μm (a–b, d, f–j, o–r, a’, b′, f′, h′–j’, l’–r’)