Fig. 4

S-phase cell distribution and live observations and in regenerates, cutting level after proventricle (L2). a–h′. EdU (pulse-chase) BrdU (pulse) stainings. a’–h. Anterior regeneration. ah′. posterior regeneration; arrowhead in c′ points to EdU chased cells at the border of wounded foregut. i–r’. Light microscopy imaging of living specimens. i–r. Anterior regeneration. i′–r’. Posterior regeneration; arrowheads in q’ and r’ point to the region with urinary concretions in the rectal intestine. White dashed lines circumscribe the shape of the animals. Black dashed lines indicate amputation site. Abbreviations: pr proventricle sci dorsal cirri of the stock individual, ve part of ventricle protruding outward. Scale bars: 100 μm (d–f), 200 μm (a–c, g–h, i–r’)