Temperature and predator-mediated regulation of plasma cortisol and brain gene expression in juvenile brown trout (Salmo trutta)

Table 2 Genes, accession numbers and primer sequences used for qPCR analysis. Asterisks denote reference genes

Gene	Accession No.	Forward primer	Reverse primer
5-HT1Aα	AGKD01067361	ATGCTGGTCCTCTACGGGCG	CGTGGTTCACCGCGCCGTTT
5-HT1Aβ	DY694524	TTGATCATGCGTTCCCAGCCGA	AAAGGAATGTAGAACGCGCCGA
AVTR	AGKD01053513	ACGGGTTCATCTGCCACAGCA	TGACAGTTCTCAATTTCGCTCTGGA
CALM	BT057678	TGCTGCAGAGCTGCGTCACG	AGCCTCCCGGATCATCTCATCCA
CRF1	NM_001141590	CACACCCACATCCTAGGCTACTCAA	TAGCGGGGTTGGAAGGCACCA
CRFR1	AKD01020666	ATCATCCATTGGAACCTGAT	ATCCAGAAGAAGTTTGTCAC
CRHBP	NM_001173799	TTGAGAAGCGTGCGGTGCGT	AGCTGCTCTCGGAAAGTCCCCT
EPD1	NM_001140909	TCTGTGAGGGTGTGGAGCTGGAG	TTGGTTGGTTGGTTGGGGCTG
GABARAP	NM_001142717	ACTCCCCCTCCTTCCCTCATCCA	ATCCCCATCTCGGCGACCCG
GRα	GQ179974	TGGCCTGTATCCCCCACTGCC	CCGCTGGGCTTGGCTGACG
GRβ	NM_001124730.1	ACGACGATGGAGCCGAAC	ATGGCTTTGAGCAGGGATAG
GRγ	NM_001124482.1	TGGTGGGCTGCTGGATTTCTGC	CTCCCTGTCTCCCTCTGTCA
GSR	BG934480	CCAGTGATGGCTTTTTTGAACTT	CCGGCCCCCACTATGAC
MHC1	AF504021	AATGGATCGCCCCAACGCCA	CTGTCGCGTGGCAGGTCACT
MR	AGKD01011423	AGCTGGCTGGGAAACAGATGA	TCAGGGTGATTTGGTCCTCTATGG
RBP1	NM_001140773.2	GTGGCGGGGCCCTACGCTAT	TCCTGTGCCCAGCATGTCGC
β-actin*	BG933897	CCAAAGCCAACAGGGAGAAG	AGGGACAACACTGCCTGGAT
EF1α*	NM_001124339.1	GCAGGAAAAGAACCCAACG	AGTTACCAGCAGCTTTCTTCC

5-HT1Aα serotonin 1a-like receptor; 5-HT1Aβ serotonin 1b-like receptor; AVTR arginine-vasotocin receptor; CALM calmodulin; CRF1 corticotropin-releasing factor 1; CRFR1 corticotropin-releasing factor receptor 1; CRHBP corticotropin-releasing factor binding protein; EPD1 ependymin; GABARAP gamma-aminobutyric acid receptor-associated protein; GRα glucocorticoid receptor; GRβ glucocorticoid receptor 1; GRγ glucocorticoid receptor 2; GSR glutathione reductase; MHC1 major histocompatibility complex 1; MR mineralocorticoid receptor; RBP1 retinol binding protein 1; β-actin β-actin; EF1α elongation factor 1α

ISSN: 1742-9994