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Table 1 Relative gene expression of liver and white skeletal muscle (WSM) of gilthead sea bream. Data are the mean ± SEM of 10 fish of different ages (+ 3, three-year-old; + 1, one-year-old) sampled in winter (W) and summer (S). P-values are the result of two-way analysis of variance

From: Local DNA methylation helps to regulate muscle sirtuin 1 gene expression across seasons and advancing age in gilthead sea bream (Sparus aurata)

 

Winter

Summer

Two-way ANOVA

W + 3

W + 1

S + 3

S + 1

Season

Age

Interaction

LIVER

sirt1

2.53 ± 0.24

3.22 ± 0.21

1.02 ± 0.08

1.52 ± 0.11

< 0.001

0.002

0.597

sirt2

4.15 ± 0.33

6.20 ± 0.46

5.21 ± 0.27

6.99 ± 0.27

0.012

< 0.001

0.697

sirt3

1.54 ± 0.16

1.60 ± 0.12

0.81 ± 0.04

0.82 ± 0.04

< 0.001

0.762

0.799

sirt4

0.59 ± 0.06

0.64 ± 0.07

0.34 ± 0.02

0.48 ± 0.03

< 0.001

0.081

0.406

sirt5

6.86 ± 0.54

10.3 ± 0.52

5.47 ± 0.21

6.83 ± 0.26

< 0.001

< 0.001

0.014

sirt6

0.69 ± 0.08

0.72 ± 0.05

0.42 ± 0.03

0.51 ± 0.05

0.001

0.531

0.935

sirt7

1.07 ± 0.08

1.17 ± 0.07

1.11 ± 0.06

1.20 ± 0.04

0.177

0.385

0.795

pgc1α

3.29 ± 0.90

5.03 ± 0.63

4.14 ± 0.33

4.75 ± 0.32

0.633

0.058

0.350

cpt1a

20.1 ± 3.21

28.7 ± 2.37

3.94 ± 0.48

8.57 ± 0.98

< 0.001

0.004

0.357

cs

30.1 ± 1.95

27.9 ± 1.18

16.3 ± 1.84

19.9 ± 1.32

< 0.001

0.666

0.077

ucp1

261.4 ± 16.1

496.2 ± 44.8

603.2 ± 45.9

1228.3 ± 105.9

< 0.001

< 0.001

0.003

WSM

sirt1

3.90 ± 0.30

1.78 ± 0.17

1.04 ± 0.09

0.77 ± 0.05

< 0.001

< 0.001

< 0.001

sirt2

3.23 ± 0.27

2.10 ± 0.14

3.44 ± 0.39

1.96 ± 0.08

0.884

< 0.001

0.488

sirt3

0.65 ± 0.06

0.58 ± 0.06

0.35 ± 0.06

0.22 ± 0.01

< 0.001

0.081

0.548

sirt4

0.60 ± 0.06

0.31 ± 0.03

0.39 ± 0.04

0.29 ± 0.03

0.017

< 0.001

0.044

sirt5

12.9 ± 1.37

5.19 ± 0.43

5.48 ± 1.02

2.72 ± 0.31

< 0.001

< 0.001

0.011

sirt6

0.55 ± 0.03

0.31 ± 0.03

0.32 ± 0.03

0.18 ± 0.02

< 0.001

< 0.001

0.092

sirt7

1.77 ± 0.16

1.00 ± 0.07

0.71 ± 0.04

0.43 ± 0.03

< 0.001

< 0.001

0.014

pgc1α

6.32 ± 0.83

4.40 ± 0.80

2.82 ± 0.27

2.55 ± 0.25

0.002

0.050

0.500

cpt1a

71.6 ± 8.58

49.2 ± 5.78

4.69 ± 0.48

9.78 ± 1.17

< 0.001

0.112

0.020

cs

235.2 ± 19.4

152.0 ± 12.8

63.9 ± 3.11

64.9 ± 4.01

< 0.001

0.002

0.006

ucp3

173.0 ± 33.1

165.1 ± 10.8

25.1 ± 1.74

46.1 ± 2.38

< 0.001

0.794

0.498