Fig. 3

Splice variants of here investigated Dmrt genes. Each coloured box represents one exon. For GmDmrt11E, the position of exon 2 and 3 is arbitrary, since genomic data are not available. The position of stop codons is marked by a red ‘X’. The DM domains and the oligomerization domain 2 (OD2a and OD2b) are indicated. Length of exons is given in base pairs. Relative position of primers used to verify splice variants and for subsequent probe synthesis are indicated: 1) long isoform of Gm-Dmrt11E; 2) probe for Gm-Dmrt11E; 3) short isoform of Gm-Dmrt11E; 4) universal probe of Tc-Dsx; 5) female-specific probe of Tc-Dsx; 6) primers used to confirm presence of isoforms A and D (*note that we could not isolate isoform C (as predicted from genomic data) from our cDNA samples); 7) probe for isoforms A/C/D; 8) probe for isoform B; 9) primers used to confirm presence of isoform B. All PCR fragments were cloned and sequenced