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Table 3 Abundance and relative proportion of sarcomeric isomyosins of the outflow segments before and after A4.1025 immunoprecipitation and A4.1025 relative affinity of the four isoforms

From: Differential expression of myosin heavy chain isoforms in cardiac segments of gnathostome vertebrates and its evolutionary implications

Protein name

Abundance

(Extract)

Abundance

(Eluted extract)

% Isoform

(Extract)

% Isoform

(Eluted extract)

A4.1025

Relative affinity

Slow myosin heavy chain 2

90803168.43

54922737.11

7.2%

15.4%

2.14

Myosin-6

195381851.59

112643694.43

15.3%

31.4%

2.04

Myosin, heavy chain 7, cardiac muscle

623452935.20

167566431.54

49.1%

46.7%

0.95

Myosin-7B-like

359536869.87

23369498.76

28.3%

6.5%

0.23

  1. Results from Myosin heavy chain isoform abundance (arbitrary units) by ESI-Quadrupole-Orbitrap of the outflow segments of S. canicula before and after immunoprecipitation with A4.1025. The A4.1025 relative affinity is the quotient between the concentration of isomyosins before and after immunoprecipitation. MYH2 and MYH6 showed more than double affinity for A4.1025 than for MYH7, and almost 10 times more affinity than for MYH7B
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Frontiers in Zoology

ISSN: 1742-9994