Differential expression of myosin heavy chain isoforms in cardiac segments of gnathostome vertebrates and its evolutionary implications

Table 1 Results from peptide sequence analysis by nano HPLC-ESI-MS/MS of the inflow and outflow segments of S. canicula

Sample	Species	Protein name	Accession no	Mass (Da)	Score	N° Sequences
S. canicula Inflow segments	C. milii	Myosin 6	V9K8H5_CALMI	179853	15683	33
	C. milii	Slow myosin heavy chain 2	V9KCZ9_CALMI	120896	12224	26
	C. milii	Myosin heavy chain 7, cardiac	V9K7A6_CALMI	223387	3702	15
	C. milii	Myosin 10-like	V9K7K0_CALMI	192725	63	3
	M. auratus	Myosin 6	MYH6_MESAU	224287	20282	32
	H. sapiens	Myosin 7	MYH7_HUMAN	223757	15031	29
	M. auratus	Myosin 7	MYH7_MESAU	223646	14126	27
	B. taurus	Myosin 2	MYH2_BOVIN	224092	13777	22
	M. musculus	Myosin 8	MYH8_MOUSE	223653	13353	21
	G. gallus	Myosin 1B	MYH1B_CHICK	223648	11536	23
	H. sapiens	Myosin 13	MYH13_HUMAN	224605	7118	18
	G. gallus	Myosin heavy chain, cardiac	MYSC_CHICK	128216	6403	14
	O. cuniculus	Myosin 7	MYH7_RABIT	85835	4908	12
	C. lupus familiaris	Myosin 9	MYH9_CANLF	227583	203	10
S. canicula Outflow segments	C. milii	Myosin 6	V9K8H5_CALMI	179853	2609	20
	C. milii	Slow myosin heavy chain 2	V9KCZ9_CALMI	120896	2516	15
	C. milii	Myosin 7B-like	V9KBQ3_CALMI	131521	1196	5
	C. milii	Myosin, heavy chain 7, cardiac	V9K7A6_CALMI	223387	1102	8
	C. milii	Myosin 7-like	V9K9K7_CALMI	149632	529	2
	C. milii	Myosin 10-like	V9K7K0_CALMI	192725	52	2
	M. musculus	Myosin 8	MYH8_MOUSE	223653	615	9
	M. musculus	Myosin 7B	MYH7B_MOUSE	222501	512	6
	M. auratus	Myosin 6	MYH6_MESAU	224287	434	10
	B. taurus	Myosin 2	MYH2_BOVIN	224092	410	9
	C. lupus familiaris	Myosin 4	MYH4_CANLF	223776	393	9
	O. cuniculus	MyHC Embryonic smooth muscle	MYSU_RABIT	58084	28	1

Results from peptide sequence analysis by nano HPLC-ESI-MS/MS of the inflow and outflow segments of S. canicula (n=3) compared with a C. milii and a Chordate database. Samples from both inflow and outflow segments contained peptides corresponding to MYH2, MYH6, MYH7, and MYH10 whereas peptides corresponding to MYH7B were only detected in the outflow segments

ISSN: 1742-9994