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Fig. 3 | Frontiers in Zoology

Fig. 3

From: Metabolic reprogramming involving glycolysis in the hibernating brown bear skeletal muscle

Fig. 3

Repression of lipid metabolism and mitochondrial oxidative phosphorylation in winter bear muscles. Protein or mRNA expression levels of fatty acid translocase (CD36), mitochondrial hydroxyacyl-coenzyme A dehydrogenase (HADH), citrate synthase (CS) and subunits of the five oxidative phosphorylation (OXPHOS) complexes (C-I to C-V) were measured using RT-qPCR and/or Western-blot analysis in vastus lateralis muscle samples from brown bears in summer (white bars) and winter (black bars) (panels A and B; N = 8-12 per season). Corresponding blots are shown in Additional file 4: Figure S2. Maximum enzymatic activities (panels A and C) for HADH, CS and cytochrome c oxidase (COX) were measured at 33 °C and 37 °C (N = 7 per season). Data are expressed as means ± sem. Statistical significance is shown for paired student t-tests (* P < 0.05; ** P < 0.01) or post-hoc Tukey tests that followed type III ANOVA (values that do not share the same superscript letter are significantly different; P < 0.05)

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