Fig. 1
From: Metabolic reprogramming involving glycolysis in the hibernating brown bear skeletal muscle
Overview of bear muscle proteomic response to hibernation. Changes in the proteome of brown bear vastus lateralis muscle between active (summer) and hibernating (winter) periods (N = 7 per season) are shown as heatmaps of differentially expressed proteins that were produced by hierarchical clustering from the MS1 quantitative-based (panel a) and 2D-DIGE-based (panel b) analyses. Signal values between animals from the two seasons were successfully discriminated (green, black and red boxes represent downregulated, intermediate and upregulated proteins, respectively). Functional annotation analysis from differential proteins revealed enriched Gene Ontology terms, which allowed determination of broad functions significantly affected by hibernation (panel c; filled circles represent the broad functions depicted by proteins that are discussed in this paper). Detailed protein abundances and fold changes are given in Additional file 1: Table S1 and Additional file 2: Table S2. AA: amino acid metabolism; Dev.: development & differentiation; ECM: extracellular matrix; Hyp.: response to hypoxia; Ox. Stress: response to oxidative stress