Fig. 10

Frontal histological sections of Zoea IV (Holländer stain). a - overview of the anterior part of the head showing the eyestalk neuropils (EyNP), and medially the most anterior part of the protocerebrum (PC), see Fig. 2C for section plane; b - section slightly posterior to A showing the ophthalmic artery (OA) dorsal to the protocerebrum (PC); c - clusters of olfactory sensory neurons (OSN, circled and labelled with black arrowheads) within the first antenna send bundles of dendrites (De, white arrowheads) into aesthetasc sensilla (AES); c`- autofluorescence image of first antenna (A1) with aesthetascs (AES); d - axons (Ax) of olfactory sensory neurons extend towards the median brain; e – putative anlage of the statocyst (St) at the base of the first antenna; f - tegumental gland (TeG) on the dorsal side of the rostrum (Ros); note that cuticle (Cu) and epidermis are detached (double arrow); g - anterior aorta (AA) in the rostrum (Ros); h – the anterior aorta (AA) opens into to the auxiliary heart, the cor frontale (CF), from which the ophthalmic artery (OA) emerges; i – the cephalic artery (CA) emerges from the cor frontale. The anterior dorsal cells (ADC) are located dorsal to the protocerebral neuropil. The stomatogastric ganglion (StG) within the cor frontale is flanked by the anterior gastric muscles (AGM); j – higher magnification slightly posteriorly than I showing the cor frontale muscles (CFM) ventral to the stomatogastric ganglion (StG); k – the cephalic artery (CA) is flanked by the anterior dorsal cells (ADC) of the protocerebrum as it penetrates into the protocerebrum. The stomatogastric nerve (StN) connects the stomatogastric ganglion to the protocerebrum (PC). The anterior part of the hematopoietic tissue (HPT) is located dorsally to the cor frontale that embraces the stomatogastric ganglion. Abbreviations: A1 - first antenna, AA - anterior aorta, ADC - anterior dorsal cells of the protocerebrum, AES - aesthetasc, AGM - anterior gastric muscle, Ax - axon, CA - cephalic artery, Cap - capillary, CF - cor frontale, CFM - cor frontale muscle, Cu - cuticle, De - dendrites, EyNP - eyestalk neuropils, HPT - hematopoietic tissue, OA - ophthalmic artery, OSN - olfactory sensory neurons, PC - protocerebrum, Ros - rostrum, St – putative statocyst anlage, StG - stomatogastric ganglion, StN - stomatogastric nerve, TeG - tegumental gland