Fig. 8
From: The flipflop orphan genes are required for limb bud eversion in the Tribolium embryo
Live imaging stills of an Tc-ff/RhoGEF2 double-RNAi embryo. a-f Developmental dynamics in a timeframe of 6 h, starting a few hours after egg lay. a Germ anlage covered by the intact serosa (S). b-f Rupture and successive retraction of extraembryonic membranes (arrows). d-e Holes form in the median regions of the embryo (dotted outline) and ingression of embryonic tissue into the yolk (arrow in f). a-f Lateral view; anterior to the left, ventral down; scale bar 100 μm