Fig. 7
From: The flipflop orphan genes are required for limb bud eversion in the Tribolium embryo
flipflop- and RhoGEF2-RNAi knockdown interferes with early embryonic morphogenesis. a Wildtype blastoderm stage displays distinction between anterior extraembryonic tissue-anlagen (serosa S) and the posterior positioned embryonic cells (E); arrows mark the border. b Fixed RNAi embryos reveal tissue constrictions in blastoderm stages during embryonic anlagen formation (arrows). c Wildtype embryo prior to axis elongation. d Young RhoGEF2 RNAi embryo with thickened posterior growth zone (arrowhead). The affected embryo is excluded from the serosa (arrow). e, h Extended germband displays an unusual S-like orientation within the yolk (y). e, inverted head appendages (f, circle) appear missing when viewed ventrally. f, g, h Median holes in ff1- and ff1/RhoGEF2 RNAi embryos (dotted line). a, c, d, e Lateral view; f-g ventral view; scale bar 100 μm; all panels in all pictures: anterior to the left; Nuclear DAPI staining