Fig. 6

Apoptosis in hypopharyngeal gland acini at pupal stage P6. Glands were fixed, labeled with fluorophore-tagged phalloidin (green) and DAPI (blue in a-d, white in a’-d’), and imaged by confocal serial sectioning. a and a’ A bundle of ductules that connects the acinus (outside the field of view at the bottom left) with the collecting duct (white asterisk). b-d Three optical planes (inter-plane distance 3.8 μm) through an acinus. Red asterisks, secretory cell nuclei; green arrows, nuclei of duct cells or accessory cells; yellow arrowheads, nuclei with condensed and fragmented chromatin (shown at a higher magnification in insets). Bars, 25 μm