Table 2 Primers used in the real-time quantitative PCR assay of gene transcription

β-actin

L08165.1

upstream: TGACGCAGATCATGTTTGAGA

60

159

downstream: GCAGAGCGTAGCCCTCATAG

GnRH-I

EF495207.1

upstream: CTGGGACCCTTGCTGTTTTG

60

232

downstream: AGGGGACTTCCAACCATCAC

GnIH

KC514473.1

upstream: ATCTACCTAGGCATGCTCCAA

58

115

downstream: ACAGGCAGTGACTTCCCAAAT

VIP

DQ023159

upstream: ACCAGTGTCTACAGCCATCTTTTG

58

204

downstream: AGGTGGCTCAGCAGTTCATCTACA

TRH

NM_001030383.2

upstream: GCAAGAGGGGCTGGAATGAT

58

133

downstream: ATGGCAGACTGCTGAAGGTC

GnRH Receptor

KJ659046.1

upstream: TCTGCTGGACCCCCTACTAC

60

127

downstream: TCCAGGCAGGCATTGAAGAG

GnIH Receptor

KF696709.1

upstream: GTCGTCATGTACACCCGCAT

56

103

downstream: TCTTGCGAGACACCTTCCTC

VIP Receptor-I

NM_001097523.1

upstream: TACTGCGTCATGGCCAACTT

58

153

downstream: TGTCCAAGCGGTGATGAACA

TRH Receptor

NM_204930.1

upstream: CTATGGCTATGTGGGGTGCC

60

189

downstream: ACTGAGGCGAAAGACCAGAC

FSH beta

EU252532

upstream: GTGGTGCTCAGGATACTGCTTCA

60

209

downstream: GTGCAGTTCAGTGCTATCAGTGTCA

LH beta

DQ023159

upstream: GACCCGGGAACCGGTGTA

58

90

downstream: AGCAGCCACCGCTCGTAG

PRL

DQ023160

upstream: TGCTCAGGGTCGGGGTTTCA

56

218

downstream: GCTTGGAGTCCTCATCGGCAAGTT

TSH beta

FJ797681.1

upstream: CTCTGTCCCAAAACGTGTGC

56

121

downstream: CCACACTTGCAGCTTATGGC

LH Receptor

XM_013192443.1

upstream: CGGATACACAACGATGCCCT

60

74

downstream: GACTCCAGTGCCGTTGAAGA

FSH Receptor

KC477215.1

upstream: CCTAGCCATTGCTGTGCATTT

60

106

downstream: TGCCAGGTTGCTCATCAAGG

3-beta HSD

KC310447.1

upstream: TGTGACGTTCCTGTACCGTG

153

downstream: TTACAACGGGTACACGCCTC

StAR

KF958133.1

upstream: GGAGCAGATGGGAGACTGGA

56

177

downstream: CGCCTTCTCGTGGGTGAT