Fig. 6

Afi-c-lectin expression at 50 and 95 % differentiation stages. A) Chromogenic WMISH of the whole 50 % regenerating arm from proximal differentiated segments to distal undifferentiated segments and terminal cap. At distal-most end staining is localized to sub-epidermal cells. In differentiating metameric units the expression expands to scattered mesenchymal cells covering the areas of future formation of oral, aboral and lateral shields as well as spines. A’) Detail of gene expression in oral shields, aboral shields, lateral shields and vertebrae. B-D) Confocal images of fluorescent WMISH of Afi-c-lectin (red) in proximal segments of 95 % differentiated arms counterstained with nuclear stain DAPI (blue). B) Maximum projection showing Afi-c-lectin expression in three proximal segments of the arm is localized to cells in the aboral shields and spines. C) Single z-plane projection through one segment in B showing detail of Afi-c-lectin expression corresponding to the shape of the growing vertebra. D) Maximum projection through one segment in B showing detail of Afi-c-lectin expression along the spine. R - right, L - left, P - proximal, D - distal