Fig. 13

Innervation of cystid wall and sole in Cristatella mucedo. a, nerve plexus of frontal cystid wall (colony view from above, duplicatures of neighbouring zooids are outlined by dashed red line) (staining with antibodies against FMRFamide); b, nerve plexus of sole (bipolar neurons are mostly seen) (staining with antibodies against FMRFamide); c, part of sole surrounded by a peripheral rim visible as a thick diagonal line (nerve plexus and two perpendicular muscle layers are clearly seen) (staining with antibodies against acetylated tubulin); d, multipolar neurons (marked by asterisks) sandwiched between two perpendicular muscular layers (some of them innervate fibers of both layers) (staining with antibodies against acetylated tubulin and phalloidin). Scale bars: 100 μm