Figure 5
Total protein and haemocyanin concentrations in haemolymph of cold and warm water octopods. Haemocyanin concentrations were calculated from the haemolymph oxygen carrying capacity, based on a molecular weight of 3.5 MDa and 70 oxygen binding sites stated for octopod haemocyanin [53]. Total protein concentration was determined according to Bradford [54]. Bars depict means + 95% C.I., n = 9–13. Differing letters indicate significant differences (P < 0.05) between octopod species for total haemolymph protein (upper case) or haemocyanin concentrations (lower case). White values on bars indicate the fraction of haemocyanin relative to total haemolymph protein and asterisks significant differences between species.