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Table 4 Gene expression analysis: gene expression (quantities) in zoea and megalopa larvae of Hyas araneus at different time points in development classified according to their function in mitochondrial energy metabolism

From: Temperature tolerance of different larval stages of the spider crab Hyas araneus exposed to elevated seawater PCO2

 

Mitochondrial energy metabolism

 

Zoea I day 0

Zoea I day 15

Zoea II day 3

Zoea II day 15

Megalopa day 3

Gene

C

C

C

CO 2

C

CO 2

C

CO 2

C

CO 2

C

CO 2

C

CO 2

C

CO 2

C

CO 2

 

10°C

20°C

10°C

10°C

20°C

20°C

10°C

10°C

20°C

20°C

10°C

10°C

20°C

20°C

10°C

10°C

20°C

20°C

PDH

1.29±0.29

1.33±0.29

1.76±0.09

1.76±0.35

 

1.20±0.23

 

1.47±0.13

 

1.51±0.04

1.49±0.12

 

0.98±0.14

1.35±0.09

1.74±0.18

1.76±0.08

 

1.78±0.18

 

1.65±0.10

 

1.28±0.08

1.05±0.01

 

1.26±0.11

0.91±0.04

IDH

1.52±0.20

2.13±0.37

1.70±0.04

2.75±0.37

 

1.79±0.09

 

2.25±0.07

 

2.02±0.12

1.89±0.07

 

1.83±0.15

 

1.66±0.06

 

1.45±0.10

1.53±0.05

 

1.17±0.06

1.64±0.05

1.45±0.08

1.39±0.04

 

1.54±0.06

1.29±0.07

NAD

1.19±0.15

1.34±0.16

0.98±0.14

1.34±0.26

 

1.05±0.05

 

1.83±0.35

0.83±0.02

0.48±0.11

 

0.73±0.18

 

0.37±0.09

0.20±0.04

1.80±0.15

1.17±0.08

1.69±0.21

0.82±0.27

1.46±0.08

0.98±0.11

1.26±0.19

 

SDH

1.55±0.15

2.07±0.41

1.69±0.10

2.31±0.29

1.84±0.12

 

2.14±0.14

 

1.37±0.08

1.58±0.06

1.11±0.09

1.34±0.03

1.77±0.15

1.54±0.05

 

1.60±0.06

 

1.60±0.06

 

1.29±0.02

1.35±0.09

 

1.38±0.04

1.31±0.07

 

CCR

1.27±0.28

2.04±0.37

1.49±0.15

2.12±0.32

 

1.68±0.13

 

1.72±0.11

 

1.43±0.12

1.36±0.12

 

0.99±0.07

1.56±0.05

1.28±0.12

1.46±0.05

 

1.65±0.14

1.64±0.08

 

1.43±0.11

1.37±0.08

 

1.57±0.12

1.16±0.05

COX

1.38±0.27

2.07±0.38

1.73±0.11

2.47±0.45

0.85±0.13

1.33±0.10

1.38±0.12

1.18±0.08

 

0.53±0.04

1.00±0.10

1.37±0.21

2.36±0.09

1.60±0.19

 

1.56±0.07

1.16±0.15

1.53±0.14

 

1.00±0.09

0.79±0.04

atpA

1.44±0.27

2.10±0.28

1.81±0.03

2.68±0.38

 

2.72±0.04

 

2.09±0.09

 

1.49±0.06

1.49±0.04

 

1.14±0.05

1.56±0.08

1.93±0.13

2.12±0.09

 

1.89±0.14

 

1.79±0.05

 

1.62±0.06

1.61±0.08

 

1.65±0.06

1.52±0.11

 
  1. Larvae were reared at control PCO2 (C) and high PCO2 (CO2) at control temperature (10°C) or exposed to a heat shock for 5 h at 20°C. Arrow direction indicates significantly higher (upwards) or lower (downwards) gene expression between CO2 treatments at the same temperature or between temperatures within the same CO2 treatment. Black arrows: CO2 effect at the same temperature (10°C or 20°C). White arrows: heat shock effect. White/Black arrows in one direction indicate a combined effect of CO2 and heat shock.