Figure 3
Whole mounts stained with polyclonal antibodies against serotonin (magenta: A, B, C, D, E), tyrosinated tubulin (green: E), and fluorophore-tagged phalloidin (cyan). Asterisks mark position of statocyst. A. Anterior end of adult specimen, projection of dorsal sections. The dorsal body-wall musculature has been omitted for clarity. Arrowhead points to swallow’s nest receptor cell. Note the position of the X-muscle ventral to the dorsal posterior commissure. B. Anterior end of adult specimen, projection of ventral sections. Arrowheads point to swallow’s nest receptor cells. Note the position of the X-muscle ventral to the statocyst. C. Dorsal projection of juvenile. Note the position of the X-muscle ventral to the dorsal posterior commissure. D. Ventral projection of juvenile. Note the position of the X-muscle ventral to the statocyst. E. Dorsal projection of juvenile. Arrows point to gland cells at posterior tip. Abbreviations: 1 dorsal neurite bundle; 2 lateral neurite bundle; 3 ventral neurite bundle; 4 medio-ventral neurite bundle; al anterior lobe; ds digestive syncytium; fr frontal ring; m mouth; om oblique inner muscle; pc dorsal posterior commissure; pl posterior lobe. Scale bars: A, B 50 μm; C, D 50 μm; E 20 μm.