Figure 5

Transcriptional activation of HATs after administration of HDAC and HAT inhibitors prior to septic injury. The transcription of histone acetyltransferase 1, histone acetyltransferase tip 60, and histone acetyltransferase type b catalytic subunit was measured by quantitative real time RT-PCR. The larvae were injected either with (A-C) HDAC inhibitors (1:1 SAHA (1 mg/ml) and sodium butyrate (20 mg/ml)) or with (D-F) HAT inhibitor (500 μg/ml), prior to injury and hemolymph loss. The expression levels were calculated relative to the expression levels in control larvae treated with 1% DMSO. Values were normalized against the 18S rRNA housekeeping gene and represent means of three independent measurements ± standard deviations (*, p < 0.05; **, p < 0.005).