Figure 2

Effect of HDAC and HAT inhibitors on the metamorphosis of G. mellonella larvae. Last-instar larvae formed a significantly greater number of pupae when injected with 1:1 SAHA (1 mg/ml) and sodium butyrate (20 mg/ml) compared to control larvae treated with 1% DMSO, whereas the injection of HAT inhibitor (500 μg/ml) delayed pupation (A). The acceleration of development by inhibition of HDACs was dose-depended as injection of SAHA in lower concentrations (400 μg/ml, 200 μg/ml or 20 μg/ml) resulted in correspondingly reduced acceleration of pupae formation (B). The larvae were incubated at 37°C on an artificial diet. Results represent mean values ± standard deviations of at least three independent measurements with 80 animals for HDAC inhibitor, HAT inhibitor or 1% DMSO treatment (*, p < 0.05; **, p < 0.005) and two experiments with total 10 animals for each concentration.