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Table 2 Sex-inducing activity of the extracts separated on a commercial octadecylsilane (ODS) column

From: Sex-inducing effect of a hydrophilic fraction on reproductive switching in the planarian Dugesia ryukyuensis (Seriata, Tricladida)

Test food

Number of test worms that developed a pair of ovaries (%)

Number of test worms that developed a copulatory apparatus (%)

Dugesia ryukyuensis

Fr. M0

50/50 (100.0 ± 0.0)

50/50 (100.0 ± 0.0)

 

Fr. M10

47/49 (96.0 ± 4.0)

44/49 (89.5 ± 6.5)

 

Fr. M30

0/48 (0.0 ± 0.0)

0/48 (0.0 ± 0.0)

 

Fr. M100

0/48 (0.0 ± 0.0)

0/48 (0.0 ± 0.0)

 

Fr. H

0/50 (0.0 ± 0.0)

0/50 (0.0 ± 0.0)

Bdelloceplala brunnea

Fr. M0

50/50 (100.0 ± 0.0)

44/50 (88.0 ± 8.0)

 

Fr. M10

45/49 (91.8 ± 4.3)

39/49 (79.6 ± 4.5)

 

Fr. M30

0/50 (0.0 ± 0.0)

0/50 (0.0 ± 0.0)

 

Fr. M100

0/48 (0.0 ± 0.0)

0/48 (0.0 ± 0.0)

 

Fr. H

0/50 (0.0 ± 0.0)

0/50 (0.0 ± 0.0)

 

Minced worms

49/49 (100.0 ± 0.0)

47/49 (96.0 ± 4.0)

Chicken liver

 

0/50 (0.0 ± 0.0)

0/50 (0.0 ± 0.0)

  1. The extracts were obtained as described in Figure 1. See Materials and methods for test food. Twenty-five test worms were also fed approximately 400 mg of minced worms and chicken liver, respectively, for 4 weeks. Means and standard errors of duplicated experiments are shown. Fr., fraction.