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Table 3 Results from fragment analysis showing the algae and krill peak height generated from PCR mixtures with different amounts of the different blocking primers added and different initial concentration of algal 28S rDNA fragments.

From: Blocking primers to enhance PCR amplification of rare sequences in mixed samples – a case study on prey DNA in Antarctic krill stomachs

Initial concentration
of Pyramimonas fragments to
Euphausia fragments
Blocking
primer
Amount of
10
μM
blocking
primer added
Peak height
    Fragment length 180
(Pyramimonas sp.)
Fragment length 199
(Euphausia superba)
1:100 No blocking 0 μL 311 8107
  Short28SR- 1.0 μL 8454 3030
   2.5 μL 8516 186
  blkKrill-3'c3 5.0 μL 8243 0
  Short28SF- 1.0 μL 8374 7538
   2.5 μL 8524 190
  DPO-blkKrill 5.0 μL 8498 66
1:1000 No blocking 0 μL 0 8301
  Short28SR- 1.0 μL 8480 6025
   2.5 μL 8470 146
  blkKrill-3'c3 5.0 μL 8617 0
  Short28SF- 1.0 μL 7740 8309
   2.5 μL 8427 318
  DPO-blkKrill 5.0 μL 8427 140
Krill only No blocking 0 μL 0 8324