Alignment and phylogenetic analysis of the ferritin isoforms. (A) The protein sequences of the identified ferritin subunits from P. dumerilii (Pdum-1, AM697674; Pdum-2, AM697675) were aligned with corresponding sequences from H. sapiens (Ferritin heavy chain, P02794; light chain, P02792; mitochondrial ferritin, Q8N4E7), S. purpuratus (Spur1, XP_001184706; Spur2, XP_796152), D. melanogaster (Dmel1, NP_524873; Dmel2, AAF07879; Dmel3, NP_572854), C. elegans (Cele1, NP_504944; Cele2, NP_491198), Suberites domuncula (Sdom1, CAC84556; Sdom2, CAC84555), Hydra vulgaris (Hydra, ABC25029), Arabidopsis thaliana (Athal, NP_195780), and Escherichia coli (Ecoli, NP_416418). Red color indicates 90% consensus and blue color 40% consensus. (B) A Bayesian protein was generated using aligned ferritin sequences including bacterial ferritin from E. coli as out-group. This analysis revealed that Platynereis ferritin 1 group near to ferritins from the sponge Suberites domuncula and the sea urchin S. purpuratus whereas the Platynereis ferritin 2 isoform groups near to the ferritins from Hydra and Drosophila. Posterior probabilities are plotted at the nodes. The scale bar represents the substitutions per site according to the model of amino acid evolution applied.