Figure 6

Contraction patterns of Tethya wilhelma during extracorporeal nitric oxide application (via release by NOC-12) (a - b): Projected area of two specimens of T. wilhelma before and during NOC-12 application (grey background). The nitric oxide released by NOC-12 induced immediate contractions in all experiments, attenuated the contraction amplitude and disturbed the rhythm of endogenous contractions (see additional file 6: Movie_S6.mov for the time-lapse movie of Fig. 6b). Filled arrowheads indicate the points in time when stock solutions of the substances were injected into the experimental reactor circulation system to reach final concentrations of 5.7 μM (a) and 22.7 μM (b) for NOC-12.