Figure 4

Organization of hemolymph sinuses in the ovipositor and their fusion with the abdominal hemocoel. Cross sections from distal to proximal, section levels indicated in figure 2C by arrows. (A) Gonapophysis 8 (ga8): note strong sclerotized lateral and flexible medial cuticle, lumen divided by septum with associated tracheae (tr) into efferent medial sinus (si8m) and afferent lateral sinus (si8l); lateral gonapophysis 9 (ga9l): efferent ventral sinus (si9v), afferent lateral sinus (si9l) and afferent dorsal sinus (si9d); the latter merges on the left side with the abdominal hemocoel (arrow); medial gonapophysis 9 (ga9m): each valve with undivided lumen; (B) left afferent si9l merges with abdominal hemocoel. (C) Ovipositor pulsatile organ 8: si8m partly filled with fat tissue; ovipositor pulsatile organ 9: the two pumping chambers are separated by invagination of ventral cuticle, compressor muscle 9 (cm9) originates laterally at coxosternite 9 (cs9) and extends to the medial cuticular invagination (arrowhead). (D) Ovipositor pulsatile organ 8: cm8 originates laterally at cs8, fans out into individual strands which extend to attachment site at medial flexible cuticle (arrow), in-between fat tissue (asterisk); ovipositor pulsatile organ 9: cm9 dorsal of si9v extending between cs9 and strong sclerotized part of medial cuticular invagination (arrowhead). Scale bars: 100 μm.