Timeline of carotenoid supplementation, blood collection, and dermal biopsies during our study. During Week 4 of the study, blood was collected from all individuals to establish initial values for circulating carotenoid, agglutination, and lysis titers prior to supplementation (Blood Collection 1). Additionally, biopsy X0 was performed during Week 4 of the study to determine the post-wounding time points with the greatest variation in wound area (to focus subsequent wound healing analyses). In Week 8 of the study, carotenoid supplementation commenced. Every three weeks during the supplementation period, blood was collected to measure circulating carotenoid levels and agglutination and lysis titers (Blood Collections 2-5). At weeks 14 and 20 of the study, we biopsied (X1, X2) the chameleons to evaluate wound healing. Wound healing rates were determined by measuring wound area from photographs taken immediately after biopsies, and on days 6 and 10 after wounding. Furthermore, we evaluated circulating nitric oxide levels by collecting blood samples immediately before, and again two days, after each biopsy.