Table 2 Primer sequences used for cDNA cloning, RACE, RT-PCR (for tissue distribution) and quantitative real-time PCR of the Odvill gene of the brackish medaka
Primer name | Primer sequence (5′ to 3′) |
|---|---|
Odvill-F | CCTGCAGATATGGACCATCAA |
Odvill-R | CAAGTCATTCTGACGCCACTT |
Odvill-RACE-F | ACACGCCAACAAGTCTGAAACAACAG |
Odvill-RACE-R | GGACGTTGTAGGAGTTTGTGTCAACG |
Odvill-RT-F | CAGCTTCAACAACGGAGACA |
Odvill-RT-R | ACCGTCACATCTCCAGAAGC |
Odvill-QPCR-F | GTTCAAGAAGTGGCCACACA |
Odvill-QPCR-R | TTGCTCTGTTCATGGCTTCTT |
β-actin-F | CTGGACTTCGAGCAGGAGAT |
β-actin-R | AGGAAGGAAGGCTGGAAGAG |