The enigma of eugregarine epicytic folds: where gliding motility originates?

Table 3 The treatment of living gregarines with cytochalasin D

Species/Cytochalasin D concentration	Gregarina cuneata			Gregarina polymorpha			Gregarina steini
	10 μM CytD	20 μM CytD	30 μM CytD	10 μM CytD	20 μM CytD	30 μM CytD	10 μM CytD	20 μM CytD	30 μM CytD
Changes/time left after drug application (in minutes)
Initial decrease of gliding speed	≥ 5 min+	≥ 5 min++	≥ 5 min+++	≥ 5 min+	≥ 5 min++	≥ 5 min+++	≥ 5 min+	≥ 5 min++	≥ 5 min+++
Further decrease of gliding speed	≥ 20 min+	≥ 10 min++	≥ 10 min+++	≥ 15 min+	≥ 10 min++	≥ 10 min+++	≥ 10 min+	≥ 10 min++	≥ 10 min+++
Cellular changes (shrivelling)	≥ 30 min	≥ 30 min	≥ 30 min	≥ 30 min	≥ 30 min	≥ 30 min	≥ 30 min	≥ 30 min	≥ 30 min
Complete stoppage of gliding motility	≤ 120 min	≤ 90 min	≤ 75 min	≤ 75 min	≤ 60 min	≤ 30 min	≤ 30 min	≤ 20 min	≤ 10 min
Recovery after washing in Ringer’s solution	≤ 1 min	≤ 1 min	≤ 1 min	≤ 1 min	≤ 1 min	≤ 1 min	≤ 1 min	≤ 1 min	≤ 1 min
Full recovery after washing in Ringer’s solution	≥ 5 min	≤ 10 min	≤ 10 min	≥ 5 min	≤ 10 min	≤ 10 min	≥ 5 min	≥ 10 min	≥ 10 min

The symbol + indicates an intensity of observed change ranging from + to +++ when compared between gregarine species and concentrations of cytochalasin D. Applied only if the listed phenomenon showed significant differences in its intensity.

ISSN: 1742-9994