Figure 4

Gene expression pattern during acclimation to acidified conditions. Gene expression time series of acid–base relevant genes, including Na⁺/H⁺-exchanger 3 (NHE3) (A), Rh protein (RhP) (B), H⁺-ATPase (VHA) (C) Na⁺/K⁺-ATPase (NKA) (D) and the Na⁺/HCO₃^- exchanger (NBC) (E) in homogenates of body (without yolk) and yolk tissues (F-J) exposed to control (solid line) and acidified (dashed line) conditions. Samples were taken at seven different time points along the incubation period of 120 h. Expression of the gene candidates are normalized to slUBC. Letters denote a significant difference between each time point whereas asterisks indicate significant differences between treatments. Values are expressed as means ± SE (n = 3).