Figure 3

Knockdown of nhe3b increases NCC cell number. (A, B): NCC (arrow) immunostaining images of 5-dpf wild type larvae (WT) and nhe3b morphants. Scale bar = 100 μm. (C): Comparison of NCC cell number in different areas between 5-dpf wild type larvae (white bars) and nhe3b morphants (gray bars) (n = 8). (D): Whole body Na⁺ content in wild type larvae (white bars) and nhe3b morphants (gray bars) at different developmental stages (n = 6). Mean ± SD. *p < 0.05; **p < 0.01; ***p < 0.001 (Student’s t-test).