Figure 2

Loss of HR cells by gcm2 knockdown increases NCC cell number and Na⁺absorption ability. (A, B): Immunostaining images of NCC cells (arrow) in 4-dpf wild type larvae (WT) and gcm2 morphants. Scale bar = 100 μm. (C): Comparison of NCC cell density between wild type larvae (white bars) and gcm2 morphants (gray bars) at different developmental stages (n = 8). (D): Na⁺ influx in 4-dpf wild types, gcm2 morphants, and foxi3a/3b morphants (n = 6). (E): Whole body Na⁺ content in wild type larvae (white bars) and gcm2 morphants (gray bars) at different developmental stages (n = 6). Mean ± SD. *p < 0.05 (Student’s t-test).