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Table 3 Template specific primers used in this study

From: Environmental monitoring using next generation sequencing: rapid identification of macroinvertebrate bioindicator species

Primer name

Sequence (‘5 to 3’)

Gene

Reference

HCO2198/ 912

TAAACTTCAGGGTGACCAAAAAATCA

COI

[77]

LCO1490

GGTCAACAAATCATAAAGATATTGG

COI

[77]

COI A for

CCHCGAATAAATAATATAAGWTTYTG

COI

This study

911

TTTCTACAAATCATAAAGATATTGG

COI

[78]

CB1

TATGTTTTACCATGAGGACAAATATC

CytB

[79]

CB322 R

GGRTTDGCDGGRATRAARTTATC

CytB

This study

CB549 R

TTCTACDGTDGCHCCAATTCA

CytB

This study

T-N-S1

TATTTCTTTCTTATGTTTTCAAAAC

CytB

[79]

  1. Primers were used for Sanger sequencing and in 454 pyrosequencing.
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Frontiers in Zoology

ISSN: 1742-9994